Results 11 to 20 of about 12,459 (136)

Functional Nitrogenase Cofactor Maturase NifB in Mitochondria and Chloroplasts of Nicotiana benthamiana

mBio, 2022
Engineering plants to synthesize nitrogenase and assimilate atmospheric N2 will reduce crop dependency on industrial N fertilizers. This technology can be achieved by expressing prokaryotic nitrogen fixation gene products for the assembly of a functional
Xi Jiang, Diana Coroian, Emma Barahona, Carlos Echavarri-Erasun, Rocío Castellanos-Rueda, Álvaro Eseverri, Jose A. Aznar-Moreno, Stefan Burén, Luis M. Rubio   +8 more
doaj   +1 more source

Rhodobacter capsulatus AnfA is essential for production of Fe‐nitrogenase proteins but dispensable for cofactor biosynthesis and electron supply

MicrobiologyOpen, 2020
The photosynthetic α‐proteobacterium Rhodobacter capsulatus reduces and thereby fixes atmospheric dinitrogen (N2) by a molybdenum (Mo)‐nitrogenase and an iron‐only (Fe)‐nitrogenase.
Lisa Demtröder, Yvonne Pfänder, Bernd Masepohl   +2 more
doaj   +1 more source

Nitrogenase resurrection and the evolution of a singular enzymatic mechanism

eLife, 2023
The planetary biosphere is powered by a suite of key metabolic innovations that emerged early in the history of life. However, it is unknown whether life has always followed the same set of strategies for performing these critical tasks.
Amanda K Garcia, Derek F Harris, Alex J Rivier, Brooke M Carruthers, Azul Pinochet-Barros, Lance C Seefeldt, Betül Kaçar   +6 more
doaj   +1 more source

GmTNRP1, associated with rhizobial type‐III effector NopT, regulates nitrogenase activity in the nodules of soybean (Glycine max)

Food and Energy Security, 2023
Soybean is a rich source of protein and oil for humans. It forms a symbiotic association with rhizobia to fix atmospheric nitrogen for its growth and development. Rhizobial type‐III effectors act as important signaling molecules in symbiosis.
Chao Ma, Chunyan Liu, Yanyu Yu, Shengnan Ma, Siyang Pan, Haojie Feng, Qingshan Chen, Dawei Xin, Xiaoxia Wu, Jinhui Wang   +9 more
doaj   +1 more source

An alternative path for the evolution of biological nitrogen fixation

Frontiers in Microbiology, 2011
Nitrogenase catalyzed nitrogen fixation is the process by which life converts dinitrogen gas into fixed nitrogen in the form of bioavailable ammonia. The most common form of nitrogenase today requires a complex metal cluster containing molybdenum (Mo ...
Eric S Boyd, Trinity L Hamilton, John W. Peters   +2 more
doaj   +1 more source

Selenocyanate derived Se-incorporation into the nitrogenase Fe protein cluster

eLife, 2022
The nitrogenase Fe protein mediates ATP-dependent electron transfer to the nitrogenase MoFe protein during nitrogen fixation, in addition to catalyzing MoFe protein-independent substrate (CO2) reduction and facilitating MoFe protein metallocluster ...
Trixia M Buscagan, Jens T Kaiser, Douglas C Rees   +2 more
doaj   +1 more source

Classifying the metal dependence of uncharacterized nitrogenases

Frontiers in Microbiology, 2013
Nitrogenase enzymes have evolved complex iron-sulfur (Fe-S) containing cofactors that most commonly contain molybdenum (MoFe, Nif) as a heterometal but also exist as vanadium (VFe, Vnf) and heterometal independent (Fe-only, Anf) forms.
Shawn E Mcglynn, Eric S Boyd, John W. Peters, Victoria J Orphan   +3 more
doaj   +1 more source

nifDK Clusters Located on the Chromosome and Megaplasmid of Bradyrhizobium sp. Strain DOA9 Contribute Differently to Nitrogenase Activity During Symbiosis and Free-Living Growth

Molecular Plant-Microbe Interactions, 2016
Bradyrhizobium sp. strain DOA9 contains two copies of the nifDK genes, nifDKc, located on the chromosome, and nifDKp, located on a symbiotic megaplasmid.
Jenjira Wongdee, Pongpan Songwattana, Nico Nouwen, Rujirek Noisangiam, Joel Fardoux, Clémence Chaintreuil, Neung Teaumroong, Panlada Tittabutr, Eric Giraud   +8 more
doaj   +1 more source

A colorimetric method to measure in vitro nitrogenase functionality for engineering nitrogen fixation

Scientific Reports, 2022
Biological nitrogen fixation (BNF) is the reduction of N2 into NH3 in a group of prokaryotes by an extremely O2-sensitive protein complex called nitrogenase. Transfer of the BNF pathway directly into plants, rather than by association with microorganisms,
Lucía Payá-Tormo, Diana Coroian, Silvia Martín-Muñoz, Artavazd Badalyan, Robert T. Green, Marcel Veldhuizen, Xi Jiang, Gema López-Torrejón, Janneke Balk, Lance C. Seefeldt, Stefan Burén, Luis M. Rubio   +11 more
doaj   +1 more source

Archaea, bacteria and termite, nitrogen fixation and sustainable plants production

Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2021
Certain bacteria and archaea are responsible for biological nitrogen fixation. Metabolic pathways usually are common between archaea and bacteria. Diazotrophs are categorized into two main groups namely: root-nodule bacteria and plant growth-promoting ...
Wenli SUN, Mohamad H. SHAHRAJABIAN, Qi CHENG   +2 more
doaj   +1 more source