Results 41 to 50 of about 1,434,331 (312)

Molecular action of NZ2114, a superior plectasin derivative

npj Antimicrobials and Resistance
NZ2114 is a triple-mutant of the natural peptide antibiotic plectasin that targets the peptidoglycan precursor molecule Lipid II using a supramolecular action, involving assembly of plectasin-Lipid II complexes in a large oligomeric structure, that is ...
Maik G. N. Derks, Shehrazade Jekhmane, Sourav Maity, Vicky Charitou, Cornelis J. Slingerland, Benjamin Vermeer, Mick van der Weijde, Charalampos Ntallis, Nathaniel I. Martin, Wouter H. Roos, Eefjan Breukink, Markus Weingarth   +11 more
doaj   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

A NMR guided approach for CsrA–RNA crystallization

, 2013
Structure determination of protein–nucleic acid complexes remains a challenging task. Here we present a simple method for generating crystals of a CsrA–nucleic acid complex, guided entirely by results from nuclear magnetic resonances spectroscopy (NMR ...
NMR Spectroscopy, Kaptein, R., Gronenborn, A.M., Sub NMR Spectroscopy, Boelens, R., Koharudin, L.M.I.   +5 more
core   +1 more source

Dose‐dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction

FEBS Open Bio, EarlyView.
NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks, Garth L. Maker, Emily C. Davies, Berin A. Boughton, Samantha Lodge   +4 more
wiley   +1 more source

Brevibacillin 2V, a Novel Antimicrobial Lipopeptide With an Exceptionally Low Hemolytic Activity

Frontiers in Microbiology, 2021
Bacterial non-ribosomally produced peptides (NRPs) form a rich source of antibiotics, including more than 20 of these antibiotics that are used in the clinic, such as penicillin G, colistin, vancomycin, and chloramphenicol.
Xinghong Zhao, Xiaoqi Wang, Rhythm Shukla, Rhythm Shukla, Raj Kumar, Markus Weingarth, Eefjan Breukink, Oscar P. Kuipers   +7 more
doaj   +1 more source

Probing molecular motion by double-quantum (13C,13C) solid-state NMR spectroscopy: Application to ubiquitin

, 2010
We demonstrate the use of two-dimensional (13C,13C) double-quantum spectroscopy to detect molecular dynamics by solid-state NMR. Data collected on tyrosine-ethylester (TEE) are in line with previously determined (1H,13C) order parameters.
NMR Spectroscopy, Baldus, M., Seidel, K., Schneider, R., Etzkorn, M., Becker, S., Sub NMR Spectroscopy, de Lange, A., Lange, A.   +8 more
core   +1 more source

Fractional Deuteration applied to biomolecular solid-state NMR spectroscopy [PDF]

, 2011
Solid-state Nuclear Magnetic Resonance can provide detailed insight into structural and dynamical aspects of complex biomolecules. With increasing molecular size, advanced approaches for spectral simplification and the detection of medium to long-range ...
NMR Spectroscopy, Baldus, M., Cukkemane, A.A., Deepak Nand, Abhishek Cukkemane, Nand, D., Becker, S., Stefan Becker, Sub NMR Spectroscopy, Cukkemane, A., Marc Baldus   +10 more
core   +1 more source

Enhancing Low‐Temperature Performance of Sodium‐Ion Batteries via Anion‐Solvent Interactions

Advanced Functional Materials, EarlyView.
DOL is introduced into electrolytes as a co‐solvent, increasing slat solubility, ion conductivity, and the de‐solvent process, and forming an anion‐rich solvent shell due to its high interaction with anion. With the above virtues, the batteries using this electrolyte exhibit excellent cycling stability at low temperatures. Abstract Sodium‐ion batteries
Cheng Zheng, Qian Yao, Yanan Sun, Hongyu Lv, Zhongchao Bai, Guoliang Zhang, Nana Wang, Jian Yang   +7 more
wiley   +1 more source

Brevibacillin 2V Exerts Its Bactericidal Activity via Binding to Lipid II and Permeabilizing Cellular Membranes

Frontiers in Microbiology, 2021
Lipo-tridecapeptides, a class of bacterial non-ribosomally produced peptides, show strong antimicrobial activity against Gram-positive pathogens, including antibiotic-resistant Staphylococcus aureus and Enterococcus spp.
Xinghong Zhao, Xiaoqi Wang, Rhythm Shukla, Rhythm Shukla, Raj Kumar, Markus Weingarth, Eefjan Breukink, Oscar P. Kuipers   +7 more
doaj   +1 more source

Characterization of a Cyclic Nucleotide-Activated K+ Channel and its Lipid Environment by Using Solid-State NMR Spectroscopy

, 2013
Voltage-gated ion channels are large tetrameric multidomain membrane proteins that play crucial roles in various cellular transduction pathways. Because of their large size and domain-related mobility, structural characterization has proved challenging ...
NMR Spectroscopy, Baldus, M., Cukkemane, A.A., Sub NMR Spectroscopy   +3 more
core   +1 more source