Results 61 to 70 of about 1,885,709 (264)

Aircraft Noise Characteristics and Metrics: A PARTNER Project 24 Report [PDF]

Day-Night Average A-weighted Sound Pressure Level (DNL) is used currently to define noise contours around airports and the 65 DNL contour is used as a criterion to determine qualification for noise insulation programs.
Massachusetts Institute of Technology, More, Shashikant Ramdas, Purdue University, Partnership for Air Transportation Noise and Emissions Reduction   +3 more
core  

Targeting the EpCAM‐AXL axis to overcome drug resistance in lung cancer

Molecular Oncology, EarlyView.
Lung cancer cells often evade therapy by hijacking signaling pathways. We reveal that cleaved EpCAM (sEpCAM) stabilizes the oncogenic protein AXL, driving NF‐κB and STAT3‐mediated chemoresistance. This EpCAM‐AXL axis identifies a high‐risk patient subset with poor prognosis.
Alexa Guerrero‐Alba, Mark Shacker, Timothy P. Fleming, Michael A. Smith, Ross M. Bremner, Narendra V. Sankpal   +5 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Annual Report for Calendar Year 2017: New England Transportation Consortium [PDF]

The New England Transportation Consortium (NETC) is a cooperative effort of the transportation agencies of the six New England States, the six New England state land grant universities, and the Federal Highway Administration (FHWA).
New England Transportation Consortium
core  

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Extremely low-frequency magnetic field exposure level for Koreans by occupational code

Emerging Contaminants, 2018
This study attempted to identify the level of exposure of 350 subjects to the extremely low-frequency magnetic field (ELF-MF). The occupational group showed significantly high levels of ELF-MF in every exposure indicator during work and transportation (p 
Joon Sig Jung, Mailan Arachchige Don Rajitha Kawshalya, In Keun Shim, Jung Min Ryu, Myung Hee Kwon, Duck Shin Park, Seung Cheol Hong   +6 more
doaj   +1 more source

Annual Report for Calendar Year 2016: New England Transportation Consortium [PDF]

The New England Transportation Consortium (NETC) is a cooperative effort of the transportation agencies of the six New England States, the six New England state land grant universities, and the Federal Highway Administration (FHWA).
New England Transportation Consortium
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