Results 71 to 80 of about 32,056 (280)

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Citizens’ exposure to predominant noise sources in agglomerations

Noise Mapping
Environmental noise directive requires Member States to produce and periodically update strategic noise maps for agglomerates in order to evaluate citizens’ noise exposure before the action plan.
Bolognese Matteo, Fredianelli Luca, Stasi Gianmarco, Ascari Elena, Crifaci Giulia, Licitra Gaetano   +5 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Towards a new European Coastal Mineral Aggregates Planning Regime

European Journal of Spatial Development, 2003
A project plan was recently presented in the Netherlands with a view to extracting the riverbed area of the Heesseltsche Uiterwaarden. This is part of the much larger Fort St. Andries project. The area of the Heeseltsche Uiterwaarden is 350 hectares and
Bert Van der Moolen, Ian Wilson
doaj   +3 more sources

Identification of Noise Tonality in the Proximity of Wind Turbines—A Case Study

Applied Sciences
This paper presents a study of the tonality of sound emitted by a wind farm into the surrounding environment. The wind turbines installed at the site have a rated power of 3.0 MW.
Wolniewicz Katarzyna, Zagubień Adam
doaj   +1 more source

Influence of some of occupational factors on the course of psoriasis in men and women

Наука и инновации в медицине, 2019
Objectives to study the influence of workplace factors affecting the course of psoriasis in men and women in Ufa. Material and methods.630 patients were assessed for the level of social status, level of education, types of work, the presence of ...
Olga R. Mukhamadeeva, Nazira H. Sharafutdinova   +1 more
doaj   +1 more source

ETC/ATNI Report 2019/8: Noise Action Plans. Managing exposure to noise in Europe.

, 2020
The Environmental Noise Directive (END, Directive 2002/49/EC) sets legally binding obligations for reduction and management of environmental noise. Based upon noise mapping results, action plans have to be drawn up for major transport sources and the largest urban areas. However, the specific types of measures included in these action plans are decided
Núria Blanes, Francisco Domingues, Maria José Ramos, Miquel Sáinz de la Maza, Jaume Fons-Esteve   +4 more
openaire   +2 more sources

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source