Results 51 to 60 of about 569,184 (264)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Semi-Automated Classification of Side-Scan Sonar Data for Mapping Sabellaria spinulosa Reefs in the Brown Bank, Dutch Continental Shelf

Journal of Marine Science and Engineering
Biogenic reefs support marine biodiversity and play a key role in a healthy marine environment. Protecting and enhancing reef-building species, such as Sabellaria spinulosa, require mapping and monitoring strategies.
Timo Constantin Gaida, Bas Binnerts, Oscar Bos   +2 more
doaj   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Transient Response of an Impacted Beam and Indirect Impact Force Identification Using Strain Measurements

Shock and Vibration, 1994
The impulse response functions (force-strain relations) for Euler–Bernoulli and Timoshenko beams are considered. The response of a beam to a transverse impact force, including reflection at the boundary, is obtained with the convolution approach using ...
Hyungsoon Park, Youn-sik Park
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Experimental Investigation of Airfoil Instability Tonal Noise Reduction Using Structured Porous Trailing Edges

Applied Sciences
Reducing the tonal noise from airfoil instabilities has attracted significant interest from the aeronautical community in the past few years. The aim of this paper is to investigate the effect of structured porous trailing edges on the tonal noise ...
Yong Wang, Kongcheng Zuo, Peng Guo, Kun Zhao, Victor Feliksovich Kopiev   +4 more
doaj   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Study on Construction Methods Optimization for Subway Sound Barrier Project

Chengshi guidao jiaotong yanjiu
[Objective] The construction of subway sound barriers is characterized by complex foundation treatment, low installation efficiency and high risk of high-altitude operations, leading to extended construction period, higher costs and hidden quality risks.
YANG Lei, WANG Lixiao
doaj   +1 more source

Noise equals control

Physical Review E
Stochastic systems have a control-theoretic interpretation in which noise plays the role of control. In the weak-noise limit, relevant at low temperatures or in large populations, this leads to a precise mathematical mapping: the most probable trajectory between two states minimizes an action functional and corresponds to an optimal control strategy ...
openaire   +3 more sources