Results 61 to 70 of about 608,210 (316)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Bayesian inference of biochemical kinetic parameters using the linear noise approximation [PDF]
, 2009 Background Fluorescent and luminescent gene reporters allow us to dynamically quantify changes in molecular species concentration over time on the single cell level.
David A Rand +14 more
core +1 more source
Automatic estimation and removal of noise on digital image
Journal of Measurement Science and Instrumentation, 2013 An spatially adaptive noise detection and removal algorithm is proposed.Under the assumption that an observed image and its additive noise have Gaussian distribution,the noise parameters are estimated with local statistics from an observed degraded image,
Tuananh Nguyen +2 more
doaj
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Metamodelling techniques for propeller array far-field noise
, 2021 The present paper proposes the development of surrogate models for the description of acoustic emission of multi-propeller configurations, like those adopted by the vast majority of the Urban-Air-Mobility concepts nowadays under investigation.
Gennaretti M. +4 more
core +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
FEBS Open Bio, EarlyView.RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
Variance component estimation for co-estimated noise parameters in GRACE Follow-On gravity field recovery [PDF]
, 2022 Temporal gravity field modelling from GRACE Follow-On deals with several noise sources polluting the observations and the system of equations, be it actual measurement noise or mis-modellings in the underlying background models.
Arnold, Daniel +3 more
core +2 more sources
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source