Results 111 to 120 of about 157,420 (243)

Egress of non-enveloped enteric RNA viruses [PDF]

, 2021
A long-standing paradigm in virology was that non-enveloped viruses induce cell lysis to release progeny virions. However, emerging evidence indicates that some non-enveloped viruses exit cells without inducing cell lysis, while others engage both lytic ...
Owusu, Irene A, Passalacqua, Karla D, Quaye, Osbourne, Wobus, Christiane E   +3 more
core   +1 more source

Purification and concentration of model viruses using single‐pass tangential flow filtration

Biotechnology Progress, EarlyView.
Single‐pass TFF was developed for an enveloped and non‐enveloped virus for use in continuous downstream purification processes. Enveloped virus was concentrated and purified. Abstract The vaccine and viral vector industry is growing at an accelerated rate.
Luis Alberto Mejía‐Manzano, Lynn Manchester, Grace Wallis, Kan Wang, Bruce P. Lee, Michael Betenbaugh, Caryn L. Heldt   +6 more
wiley   +1 more source

Status and future of recombinant adeno‐associated virus vector manufacturing

Biotechnology Progress, EarlyView.
Abstract Sixty years of adeno‐associated virus (AAV) research illustrates a trajectory marked by basic science exploration, iterative innovation, persistent challenges, a number of clinical setbacks, as well as commercial therapeutic triumphs. This continual evolution has led to recombinant AAV (rAAV) becoming a cornerstone of modern gene therapy ...
Frank Agbogbo, David Dismuke
wiley   +1 more source

Demonstration of nanofiltration‐based virus safety for AAV manufacturing using model viruses

Biotechnology Progress, EarlyView.
Abstract Virus safety for biopharmaceutical products has been a well‐established concern for several decades. While various virus reduction steps have been identified and implemented for traditional plasma‐derived and monoclonal antibody products, there are various challenges to achieving the same virus safety in emerging gene therapy products.
Brian Buesing, Tomoki Igi, Daniel Strauss   +2 more
wiley   +1 more source

Serial femtosecond X-ray diffraction of enveloped virus microcrystals [PDF]

, 2015
Serial femtosecond crystallography (SFX) using X-ray free-electron lasers has produced high-resolution, room temperature, time-resolved protein structures.
Chelsie E. Conrad, Vadim Cherezov, Thomas D. Grant, Jesse Coe, Uwe Weierstall, John C. H. Spence, Haiguang Liu, Daniel James, Ganesh Subramanian, Brenda G. Hogue, Sébastien Boutet, Nadia A. Zatsepin, Garrett Nelson, Petra Fromme, Mark S. Hunter, Robert M. Lawrence, Mengning Liang, Andrew Aquila, Wei Liu   +18 more
core   +1 more source

A multiplex interactome of Ebola virus proteins reveals TM9SF2 as a cell‐surface attachment factor that promotes viral entry

iMetaOmics, EarlyView.
This study generates a comprehensive Ebola virus (EBOV)‐human protein–protein interactome, comprising 1728 core high‐confidence interactions. Further interactome analysis revealed the potential association of EBOV glycoprotein (GP) with the host factor TM9SF2. Subsequent mechanistic investigations confirmed that TM9SF2 functions as an attachment factor
Limin Shang, Shouwen Du, Yuchen Liu, Letian Li, Xi Wu, Yanzhi Yuan, Chaozhi Jin, Qingkun Mi, Yuhang Wang, Xiaoshuang Shi, Jiamin Wang, Guoqing Zhang, Yidan Ma, Weijin Huang, Jigang Wang, Jian Wang, Chang Li   +16 more
wiley   +1 more source

Regulation of Interferon Stimulated Genes Following Enveloped Virus Entry and Delivery of Viral Nucleic Acid [PDF]

, 2020
Innate antiviral defence depends on virus recognition and cytokines like interferon (IFN) that upregulate many interferon-stimulated genes (ISGs). Virus recognition normally relies on pattern recognition receptors binding to virus-associated nucleic acid
Hare, David
core  

A Novel Air‐Liquid Interface Exposure System for Continuous Gas Exposure of Airway Epithelial Cells: Exposure of NO2 With Viral Co‐Challenge

Journal of Applied Toxicology, EarlyView.
ABSTRACT Most in vitro toxicological research investigating the effects of inhaled compounds employ short‐term exposures of a couple of hours, or repeated, intermittent exposures for a limited number of days with exposure free periods, whereas in reality, we are continuously exposed, albeit to variable concentrations.
Marcia M. Oldenburger, Evert Duistermaat, Pieter S. Hiemstra, Flemming R. Cassee, Yvonne C. M. Staal   +4 more
wiley   +1 more source

A gammaherpesvirus complement regulatory protein promotes initiation of infection by activation of protein kinase Akt/PKB. [PDF]

, 2010
Viruses have evolved to evade the host's complement system. The open reading frames 4 (ORF4) of gammaherpesviruses encode homologs of regulators of complement activation (RCA) proteins, which inhibit complement activation at the level of C3 and C4 ...
Stewart, James P., Stipan, J., Adler, Heiko, James P Stewart, Adler Barbara, Adler, Barbara, Heiko Adler, Beatrix Steer, Barbara Adler, Jonjic Stipan, Stipan Jonjic, Steer, Beatrix, Steer Beatrix, Steer, B., Adler Heiko, Stewart James P., Adler, H., Adler, B., Jonjic, Stipan, James P. Stewart, Stewart, J.P.   +20 more
core   +1 more source

Binding and entry of a non-enveloped T=4 insect RNA virus is triggered by alkaline pH

Virology, 2016
Tetraviruses are small, non-enveloped, RNA viruses that exclusively infect lepidopteran insects. Their particles comprise 240 copies of a single capsid protein precursor (CP), which undergoes autoproteolytic cleavage during maturation. The molecular mechanisms of capsid assembly and maturation are well understood, but little is known about the viral ...
Penkler, David L, Jiwaji, Meesbah, Domitrovic, Tatiana, Short, James R, Johnson, John E, Dorrington, Rosemary A   +5 more
openaire   +3 more sources