Results 71 to 80 of about 13,141,647 (272)
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
The impact of prenatal exposure to trace metal mixtures on children’s neurodevelopment remains debated. Many studies treat all trace metals as a single entity, overlooking the distinct biological roles of essential and toxic metals.
Xiruo Kou +5 more
doaj +1 more source
Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer +7 more
wiley +1 more source
The interaction between metals and catecholamines plays a pivotal role in the generation of reactive oxygen species (ROS), leading to oxidative stress and DNA damage.
Elena Cassera +3 more
doaj +1 more source
Journal of the Institute of Metals.
Monthly issues lack numbering.Vol. 12 accompanied by supplement.Mode of access: Internet.Issued 1966 with v. 11 of: Metallurgical reviews, later issued with: Metals & materials and metallurgical reviews.Issued Sept.
Institute of Metals.
core
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source
Industrial waste and urban waste containing heavy metals can pollute rivers and sea waters. Heavy metals can be divided into two parts, namely the essential and non essential, nonessential heavy metals can be toxic to both human beings and fish.
Agustono Agustono +2 more
doaj +1 more source
A minimal cellulosome‐like system in Cellulosilyticum lentocellum
Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan +2 more
wiley +1 more source
The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav +4 more
wiley +1 more source
Effect of toxic metals on oral tissues
Toxic metals that commonly occur in human environment may exert a negative influence on people’s health. This study presents the problem of pathological changes occurring within the oral cavity as a result of exposure to toxic metals.
Katarzyna Herman +7 more
doaj +3 more sources

