Results 61 to 70 of about 10,355,181 (279)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

دراسة تاثير المتضمنات اللامعدنية على مطروقات الفولاذ واطئ التسبيك

Journal of Engineering and Sustainable Development, 2022
The non-metallic inclusions can be found in steel as a result of existence of oxygen and sulpher in form of oxides and sulphides which they have relative low solubility.
علي حسين عتيوي, ستار عبود عباس, صباح عجيب كاصد   +2 more
doaj  

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Rotating bending fatigue behavior of medium carbon Cr and W nitriding bearing steel

Journal of Hebei University of Science and Technology, 2018
Aiming at the problem that the failure mode of bearing steels is mainly fatigue failure, RBF (rotating bending fatigue) test of medium carbon Cr and W nitriding bearing steel and analysis are conducted for the medium carbon Cr and W nitriding bearing ...
Shichao CHENG, Huijun WEI, Shiqing SUN, Song WANG, Ruiyang WANG, Boshuai CUI, Nianlun WEI   +6 more
doaj   +1 more source

A minimal cellulosome‐like system in Cellulosilyticum lentocellum

FEBS Open Bio, EarlyView.
Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan, Amias Alstrom‐Moore, Gary W Black   +2 more
wiley   +1 more source

Studying microstructure of heat resistant steel deoxidized by barium ferrosilicon

Metalurgija, 2016
The paper examined the nature and distribution of non-metallic inclusions in the heat-resistant steel 12H1MF (0,12 % С, 1 % Сr, 0,5 - 0,6 Mo, 0,5 % V), ferrosilicobarim. As a reference, used by steel, deoxidized silicon.
A. Z. Issagulov, Sv. S. Kvon, V. Y. Kulikov, N. B. Aitbayev   +3 more
doaj  

Study on Cleanliness Controlling of Liquid of Low Carbon Al-killed 0.20 C Steel

Teshugang, 2022
In order to study the control technology of nonmetallic inclusions in aluminum deoxidized 0. 20 C steel (/% ：0. 13 ~0.23C,0. 17 〜0.37Si,0.35 ~0.65Mn, ≤ 0.035P, ≤ 0.035S,0.020 - 0.050Al) produced by the process of “120 t BOF-LF-RH-160 mm x 160 mm billet ...
屠兴圹, 徐建飞, 周淼, 苏振伟, 林俊, 万文华   +5 more
doaj  

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source

INCREASE STRUCTURAL STRENGTH OF MARGANESE STEEL

Nauka ta progres transportu, 2011
The manganese steels are widely used in machinery construction. The influence of chemical composition and form of non-metallic inclusions on constructive strength of manganese steels is studied.
L. N. Bunina, I. P. Volchok
doaj