Results 151 to 160 of about 30,589,948 (306)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

The Role Of Additional Employee Income In Moderating The Effect Of Work Discipline, Charismatic Leadership, And Non-Physical Work Environment On Work Productivity

EKOMBIS REVIEW: Jurnal Ilmiah Ekonomi dan Bisnis
This study aims to examine the effect of work discipline, charismatic leadership, and non-physical work environment on work productivity, as well as the moderating role of additional employee income. The research population included 60 employees of the Regional Secretariat of Musi Rawas Regency with a saturated sampling technique.
Ahmad Basri, Mulyadi Mulyadi, Rudi Hermantoni   +2 more
openaire   +1 more source

Salmonella enterica serovar typhi limits the potency of typhoid toxin and ADP‐ribosylating toxin AB to establish a persistent infection

FEBS Open Bio, EarlyView.
The two catalytic subunits of typhoid toxin dissociate from the holotoxin in the ER of an intoxicated cell, but only CdtB exits the ER to generate immunosuppressive effects. PltA is retained in the ER and sequestered from its cytosolic target, thus allowing the anti‐inflammatory effects of CdtB to promote intestinal colonization.
Maria C. Zabala‐Rodriguez, Juliette K. Tinker, Teresa Frisan, Taj Azarian, Ken Teter   +4 more
wiley   +1 more source

ANALYSIS OF PHYSICAL WORK ENVIRONMENT, NON-PHYSICAL WORK ENVIRONMENT, AND TRANSFORMATIONAL LEADERSHIP ON EMPLOYEE PERFORMANCE OF COMOP 2 AND COMOP 3 AT PT X

Journal of Social and Economics Research
PT X, as a national geotechnical consulting company, faces challenges in maintaining optimal employee performance in the COMOP 2 and COMOP 3 Departments. The emerging phenomenon is the fluctuation in productivity and work quality, which is suspected to be influenced by the physical work environment, non-physical work environment, and the leadership ...
null Ahmad Syahrir Ridwan, null Dimas Nugroho Dwi Seputro   +1 more
openaire   +1 more source

The Influence of Physical and Non-Physical Work Environment on Employee Performance at Hilton Bali Resort

This research investigates how the physical work environment (X1) and the non-physical work environment (X2) relate to employee performance (Y) at Hilton Bali Resort. A quantitative causal-associative design was applied, with data obtained from a digital
Yulia Tria Hapsari, I Nyoman Surya Abdi Wibawa, Nyoman Indah Kusuma Dewi   +2 more
core   +1 more source

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source

The Relationship between Physical Work Environment, Non-Physical Work Environment, and Organizational Commitment on the Performance of Nurses in the Inpatient Ward of Kendari City Hospital

Journal of Health Science and Medical Therapy
Nurse performance is the effort of nurses in carrying out their duties and responsibilities to the best of their abilities, in order to achieve the main goals of the profession and organizational objectives. The purpose of this study is to examine the physical work environment's impact on the performance of nurses in the Inpatient Ward of Kendari City ...
Siti Nuraena, Nani Yuniar, Fikki Prasetya   +2 more
openaire   +1 more source

MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation

FEBS Open Bio, EarlyView.
MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima, Kotone Watanabe, Grigorii Sultanakhmetov, Aoi Fukuchi, Keiya Ito, Sawako Shimizu, Taro Saito, Akiko Asada, Kanae Ando   +8 more
wiley   +1 more source

THE INFLUENCE OF PHYSICAL WORK ENVIRONMENT, NON-PHYSICAL WORK ENVIRONMENT, WORKLOAD AND TRANSFORMATIONAL LEADERSHIP STYLE ON CV EMPLOYEE PERFORMANCE. PUTRA PERKASA JAYA

Dynamic Management Journal
Company really needs efforts that can improve employee performance to achieve or even exceed the targets set by the company. This study aims to determine and analyze the influence of the physical work environment, non-physical work environment, workload and transformational leadership style on employee performance at CV.
Gilang Gautama, Pawestri Winahyu, Tatit Diansari Reskiputri   +2 more
openaire   +1 more source