Results 131 to 140 of about 30,589,948 (306)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

The quality of life of community health agents and possible contributions of occupational therapy

Cadernos Brasileiros de Terapia Ocupacional, 2018
Introduction: Community Health Agents (CHAs) are a link between the health team and the community. Their work may cause suffering and illness, harming their Quality of Life which is understood as the individual’s perception of their life position in ...
Amanda Maria Pereira, Adriana Lobo Jucá, Ivo de Andrade Lima, Vera Lucia Dutra Facundes, Ilka Veras Falcão   +4 more
doaj   +1 more source

THE EFFECT OF NON-PHYSICAL WORK ENVIRONMENT AND WORK STRESS ON THE PERFORMANCE OF AUTO 2000 EMPLOYEES OF RADEN INTAN

International Journal of Accounting, Management, Economics and Social Sciences (IJAMESC)
The influence of the non-physical work environment and stress levels on employee performance are the main objectives of this research, especially in the organizational environment of Auto 2000 Raden Intan, a corporate entity operating in the automotive industry sector. This study used a quantitative approach.
null Hendri Dunan, null Jenita Sari
openaire   +1 more source

Pengaruh Lingkungan Kerja Fisik, Disiplin dan Motivasi Terhadap Kinerja Karyawan Pada PT Asuransi Bangun Askrida Cabang Medan

The aim of this research is to determine the influence of the Physical Work Environment, Discipline and Motivation on Employee Performance at PT Asuransi Bangun Askrida Medan Branch.
Efendi, Syahrial, Aginta, Windy, Surbakti, Syamsul Bahri, Dewi, Sri Puspa, Daulay, Akman   +4 more
core   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

The Influence of Physical Work Environment and Non-Physical Work Environment and Work Culture on Teacher Performance With Discipline as an Intervening Variable in High Schools of Paser District

International Journal of Asian Business and Management
This research aims to determine the influence of the physical work environment and non-physical work environment as well as work culture on teacher performance with discipline as an intervening variable at the Paser District Public School. The research design used in this research is descriptive quantitative.
null Elli Rahmawati, null Ratno Purnomo, null Meirani Harsasi   +2 more
openaire   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Broad background to the physical environment

, 2001
The physical environment is the set of physical and biological conditions that surround human beings at the Earth's surface. This book is broadly based on a systems approach to the physical environment.
Spronken-Smith, R.A., Sturman, A.P.
core  

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source