Optimized RT-qPCR and a novel normalization method for validating circulating miRNA biomarkers in ageing-related diseases [PDF]
Scientific Reports, 2023 Circulating miRNAs have potential as minimally invasive biomarkers for diagnosing various diseases, including ageing-related disorders such as Alzheimer’s disease (AD).
Andrew Want +8 more
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Selecting Normalizers for MicroRNA RT-qPCR Expression Analysis in Murine Preimplantation Embryos and the Associated Conditioned Culture Media [PDF]
Journal of Developmental Biology, 2023 Normalizing RT-qPCR miRNA datasets that encompass numerous preimplantation embryo stages requires the identification of miRNAs that may be used as stable reference genes.
David C. Hawke +2 more
doaj +2 more sources
MiR-151a: a robust endogenous control for normalizing small extracellular vesicle cargo in human cancer [PDF]
Biomarker Research, 2023 Small extracellular vesicles (sEVs) in the blood of cancer patients contain higher amounts of tumor markers than those identified as free-circulating.
Miranda Burdiel +17 more
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Identification of extremely GC-rich micro RNAs for RT-qPCR data normalization in human plasma [PDF]
Frontiers in Genetics, 2023 We aimed at extending the repertoire of high-quality miRNA normalizers for reverse transcription-quantitative PCR (RT-qPCR) of human plasma with special emphasis on the extremely guanine-cytosine-rich portion of the miRNome. For high-throughput selection
Volker Baumann +7 more
doaj +2 more sources
Advances in algorithms for normalizer gene selection in qRT-PCR: implications for cancer biology and precision medicine [PDF]
Frontiers in GeneticsQuantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) plays a significant role in gene expression analysis in cancer research and precision medicine.
Abhay Kumar Pathak +4 more
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Normalizers of intermediate congruence subgroups of the Hecke subgroups
Open Mathematics, 2017 For a square-free positive integer N, we study the normalizer of ΓΔ(N) in PSL2(ℝ) and investigate the group structure of its quotient by ΓΔ(N) under certain conditions.
Chang Heon Kim
exaly +2 more sources
Identification of SnRNA U6 as an endogenous reference gene for normalization of MiRNA expression data in COVID-19 patients [PDF]
Scientific ReportsSARS-CoV-2, the virus responsible for COVID-19, exhibits structural differences compared to other coronaviruses that impact transmission dynamics and host response.
Íris Terezinha Santos de Santana Silva +10 more
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Wastewater Surveillance for Group A Streptococcus pyogenes in a Small City [PDF]
PathogensStreptococcus pyogenes is a bacterial pathogen known to be the causative agent in many different illnesses, with Group A Streptococcus (GAS) pharyngitis (strep throat), being one of the more prevalent.
Olivia N. Birch +2 more
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Data normalization of plasma miRNA profiling from patients with COVID-19 [PDF]
Scientific ReportsWhen using the reverse-transcription quantitative polymerase chain reaction (RT-qPCR) technique for quantitative assessment of microRNA (miRNA) expression, normalizing data using a stable endogenous gene is essential; however, no universally adequate ...
Julia Tiemi Siguemoto +12 more
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cdev: a ground-truth based measure to evaluate RNA-seq normalization performance [PDF]
PeerJ, 2021 Normalization of RNA-seq data has been an active area of research since the problem was first recognized a decade ago. Despite the active development of new normalizers, their performance measures have been given little attention. To evaluate normalizers,
Diem-Trang Tran, Matthew Might
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