Results 151 to 160 of about 2,338,916 (290)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Construction of norms for the Professional Identity Scale of Nursing Undergraduates in China

Huli yanjiu
ObjectiveTo establish norms for the Professional Identity Scale of nursing undergraduates in China.MethodsUsing the Professional Identity Scale for nursing students as the assessment tool,a stratified cluster sampling method was adopted to survey 26 108 ...
LI Xiaohua, ZHU Hong, LIU Yu, TIAN Runxi, GAO Ning   +4 more
doaj  

Associations between inclusivity norms and tolerance, contact, and cooperation amid polarization: Evidence from 12 European countries. [PDF]

PNAS Nexus
Schäfer LF, Tausch N, Bukowski M, Jaspers E, Lubbers MJ, van Zalk M, Ciordia A, Potoczek A, Estevan-Reina L, Shani M, Simons JW, Friehs MT, Gurbisz D, Middendorf W, Schäfer SJ, Ullenboom J, Graf S, Hjerm M, Lavest C, Jasinskaja-Lahti I, Kende A, Petkanopoulou K, Prati F, Christ O.   +23 more
europepmc   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

Psychological determinants of spring festival homecoming: attitude ambivalence moderates the effect of subjective norms. [PDF]

Front Psychol
Xie H, Du J, Zheng X, Xiang Y.
europepmc   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source

Global Cultural Change and Anxiety in Children and Adolescents: Analyzing Socialization Goals Over Three Decades in 70 Countries. [PDF]

Dev Sci
Kulisch LK, Domínguez Rojas AL, Schneider S, Voigt B.   +3 more
europepmc   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Factors associated with the intention to donate blood among secondary school students in East Central Uganda: an application of the theory of planned behavior. [PDF]

Health Psychol Behav Med
Akulume M, Kisakye AN, Nankya FR, Kiwanuka SN.   +3 more
europepmc   +1 more source