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Two Birds With One Stone: RNA Virus Strategies to Manipulate G3BP1 and Other Stress Granule Components. [PDF]
Wiley Interdiscip Rev RNAFirdaus MER +3 more
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Phosphorylation of sindbis virus nsP3 in vivo and in vitro
Virology, 1990nsP3 is one of four viral nonstructural proteins required for RNA replication of Sindbis virus. In this report, post-translational modifications of nsP3 which occur in both vertebrate and mosquito cell cultures have been examined. In pulse-chase experiments, analyzed by immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis ...
Li, Guangpu +4 more
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Structure and function of rotavirus nonstructural protein NSP3
1996The genomes of viruses in the family Reoviridae consist of segmented double-stranded RNA. There are 10 to 12 segments depending on the genus. The 5' ends and the 3' ends of the RNAs present conserved motifs for each virus genus. These conserved motifs have been hypothesized to play a role in genomic segment assortment during virus morphogenesis.
Poncet, Didier, Aponte, C., Cohen, J.
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A Method to Monitor Activity of SARS-CoV-2 Nsp3 from Cells
2022SARS-CoV-2 protease Nsp3 is a therapeutic target for developing anti-SARS-CoV-2 drugs. Nsp3 is a large multi-spanning membrane protein, and its characterization in vitro has been challenging. Here we describe an in vitro assay to characterize the biochemical activity of full-length Nsp3 isolated from cells.
Christopher, Rainville +2 more
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Investigation of Binding Phenomenon of NSP3 and p130Cas Mutants and Their Effect on Cell Signalling
Cell Biochemistry and Biophysics, 2013Members of the novel SH2-containing protein (NSP3) and Crk-associated substrate (p130Cas) protein families form a multi-domain signalling platforms that mediate cell signalling process. We analysed the damaging consequences of three mutations, each from NSP3 (NSP3(L469R), NSP3(L623E), NSP3(R627E)) and p130Cas (p130Cas(F794R), p130Cas(L787E), p130Cas ...
, Balu K +3 more
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Function and structure of rotavirus NSP3
2008International ...
Becker, Mm +6 more
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Regulation of gene expression by the NSP1 and NSP3 non-structural proteins of rotavirus
Archives of Virology, 2011The role of the rotavirus non-structural proteins NSP1 and NSP3 in regulating cellular and viral mRNA translation has been investigated by examining the effect of added recombinant NSP3 on protein translation in a T7-based in vitro coupled transcription-translation system.
K T, Chung, M A, McCrae
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Characterization of SARS-CoV-2 protease NSP3 and NSP5 degradome.
Infectious diseases are serious threats to human health and economy in both developed and non-developed countries. They are the cause of approximately half of the death toll worldwide. Recently, a severe acute respiratory syndrome, named COVID-19 (coronavirus disease 2019), has spread across the globe and has been found to be caused by a novel strain ...openaire +1 more source