FEBS Open Bio, EarlyView.Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley +1 more source
An optimized permeabilization step for flow cytometry analysis of nuclear proteins in myeloid differentiation of blood cells into neutrophils. [PDF]
MethodsX, 2019 Viryasova GM +5 more
europepmc +1 more source
Studies of Nuclear Acidic Proteins
, 1971 C S Teng, Ching-Sung Teng, V. G. Allfrey
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Nuclear Mutants of Neurospora crassa Temperature-Sensitive for the Synthesis of Cytochrome aa3. Mitochondrial Protein Synthesis and Analysis of the Polypeptide Composition of Cytochrome c Oxidase [PDF]
, 1979 Frank E. Nargang +2 more
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Interaction of class III cellobiose dehydrogenase with lytic polysaccharide monooxygenase
FEBS Open Bio, EarlyView.The activity of lytic polysaccharide monooxygenase (LPMO) is supported by its auxiliary enzyme cellobiose dehydrogenase (CDH). The catalytic activity of both enzymes is coupled by electron transfer and a cyclic cascade generating substrates for both enzymes – hydrogen peroxide for LPMO and oxidized and non‐oxidized cellobiose and cello‐oligosaccharide ...
Angela Giorgianni +4 more
wiley +1 more source
Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions. [PDF]
J Vis Exp, 2018 Zheng X +7 more
europepmc +1 more source
FEBS Open Bio, EarlyView.Caloric restriction that extends lifespan induces the expression of PGC‐1α and MIPEP in white adipose tissue. In this study, co‐overexpression of Pgc‐1α and Mipep upregulated the gene expression of PHOSPHO1. These findings provide new insights into mitochondria‐related mechanisms underlying the effects of caloric restriction in adipocytes.
Mamiko Ishimatsu +9 more
wiley +1 more source
Myosin VI in the nucleus of neurosecretory PC12 cells: Stimulation-dependent nuclear translocation and interaction with nuclear proteins. [PDF]
Nucleus, 2018 Majewski L +8 more
europepmc +1 more source
Instability of rat liver chromatin and other nuclear non-histone proteins in alkaline solution [PDF]
, 1976 Michael Gronow, T. Thackrah, F A Lewis
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Liver‐specific lncRNAs associated with liver cancers
FEBS Open Bio, EarlyView.Long non‐coding RNAs (lncRNAs) are regulatory molecules with various functions. They are more tissue‐specific than proteins and can be used as potential biomarkers, particularly in cancer diagnostics and prognosis. In this review, we have systematically compiled all lncRNAs with exclusive expression in the human liver, verified their liver specificity ...
Olga Y. Burenina +3 more
wiley +1 more source