Results 271 to 280 of about 1,026,366 (312)
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Characterization of a Sperm Nuclear Protein
American Journal of Reproductive Immunology, 1996PROBLEM: The molecular identity of sperm DNA‐binding structural proteins contributing to the integrity of a sperm residual high salt/nuclease resistant nuclear structure is studied by cDNA cloning and monoclonal antibodies to the recombinant polypeptide.
Malcho G. Petrov+3 more
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An inhibitor protein of nuclear protein kinases
Nature, 1979THE cyclical phosphorylation and dephosphorylation of proteins, catalysed by protein kinases and phosphoprotein phosphatases, respectively, are important ways in which cells regulate many of their metabolic activities. Cells seem to have at least two distinct phosphorylation systems, one in the cytoplasm, the other in the nucleus.
Francoise Farron-Furstenthal+1 more
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Nuclear Protein Transport Pathways
Nephron Experimental Nephrology, 1999Nuclear proteins like transcription factors and ribosomal proteins are synthesized in the cytoplasm and have to be transported into the nucleus to fulfill their functions. The transport of proteins >20–60 kD through the nuclear pore complex (NPC) into the nucleus is an active, energy-requiring process.
Enno Hartmann+2 more
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Identity of nuclear membrane and non-histone nuclear proteins
Biochemistry, 1976The fate of plasma and nuclear membrane polypeptides in preparations of acidic chromosomal protein from chicken erythrocytes has been investigated. It is shown that detergent extraction procedures (Nonidet P-40, Triton X-100, and saponin), commonly employed in the preparation of acidic chromosomal protein, cannot be relied upon to remove plasma and ...
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Studies of the nuclear residual proteins
Archives of Biochemistry and Biophysics, 1968Abstract The nuclear residual proteins of rat liver have been prepared by solubilization with sodium deoxycholate and Sephadex gel filtration. These proteins were examined by analytical ultracentrifugation, DEAE-cellulose chromatography, starch gel electrophoresis, amino acid composition, and alkali-labile phosphorus analysis.
V. Patel+7 more
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Identification of a nuclear protein matrix
Biochemical and Biophysical Research Communications, 1974Abstract The structural framework of the rat liver nucleus has been identified and consists of a nuclear protein matrix. This matrix is 98.4% protein, 0.1% DNA, 1.2% RNA, and 0.5% phospholipid. The nuclear protein matrix is composed primarily of three acidic polypeptide fractions in the molecular weight range of 60–70,000 daltons.
Ronald Berezney, Donald S. Coffey
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Protein synthesis in nuclear residual protein
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 19651. The incorporation of radioactive amino acids into the nuclear residual protein from calf thymus has been investigated in vitro. 2. The nuclear residue has been found to incorporate actively [14C]tryptophan into its protein. 3. Optimal incorporation of [14C]tryptophan was obtained in the presence of Mg2+, adenosine 5′-triphosphate and an ...
Gordhan Patel, T. Y. Wang
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2007
Entry into the eukaryotic cell nucleus occurs through multiple pathways involving specific targeting signals, and intracellular receptor molecules of the importin/karyopherin superfamily which recognise and dock the nuclear import substrates carrying these signals at the nuclear pore.
Jade K. Forwood, David A. Jans
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Entry into the eukaryotic cell nucleus occurs through multiple pathways involving specific targeting signals, and intracellular receptor molecules of the importin/karyopherin superfamily which recognise and dock the nuclear import substrates carrying these signals at the nuclear pore.
Jade K. Forwood, David A. Jans
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Nuclear basic proteins in spermiogenesis
Biochimie, 1998In animal species, spermiogenesis, the late stage of spermatogenesis, is characterized by a dramatic remodelling of chromatin which involves morphological changes and various modifications in the nature of the nuclear basic proteins. According to the evolution of species, three situations can be observed: a) persistence of somatic histones or ...
Philippe Chevaillier+3 more
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Immunolocalization of Nuclear Proteins [PDF]
Immunohistochemistry permits the localization at the tissue and subcellular level of antigens for which specific antisera or monoclonal antibodies are available. A wide variety of methods are used for this purpose, and the best method for any particular application must unfortunately be determined empirically.
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