Results 221 to 230 of about 418,889 (263)

Nuclear Equivalence, Nuclear Transfer, and the Cell Cycle

Cloning, 1999
The last 20 years have seen the development of techniques for the production of mammals by nuclear transfer. Originally limited to the swapping of pronuclei and the use of early cleavage-stage embryos as nuclear donors, nuclear transfer came of age in 1995 with the birth of 2 Welsh Mountain lambs, Megan and Morag, that were produced using cultured ...
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Nuclear Transfer and Transgenesis in the Pig

2014
Somatic cell nuclear transfer (SCNT) using genetically modified donor cells facilitates the generation of tailored pig models for biomedical research and for xenotransplantation. Up to now, SCNT is the main way to generate gene-targeted pigs, since germ line-competent pluripotent stem cells are not available for this species.
Mayuko, Kurome, Barbara, Kessler, Annegret, Wuensch, Hiroshi, Nagashima, Eckhard, Wolf   +4 more
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Somatic Reprograming by Nuclear Transfer

2020
Somatic cell nuclear transfer (SCNT) is a powerful technique, although challenging, to study reprograming into the totipotent state of differentiated nuclei in mammals. This procedure was initially applied in farm animals, then rodents, and more recently in primates. Nuclear transfer of embryonic stem cells is known to be more efficient, but many types
Brochard, Vincent, Beaujean, Nathalie
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Cloning of Mice by Nuclear Transfer

Cloning and Stem Cells, 2003
ABREAKTHROUGH was made by Wilmut and his colleagues in 1996. A sheep named Dolly was successfully cloned from an adult somatic cell (Wilmut et al., 1997). In this experiment, the donor cell and enucleated oocyte were fused together as the means of nuclear transfer. After that, in 1998, Wakayama et al.
Shaorong, Gao, Michelle, McGarry, Keith E, Latham, Ian, Wilmut   +3 more
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Nuclear Transfer in Mammalian Embryos

1990
Publisher Summary This chapter discusses the nuclear transfer in mammalian embryos. Transfer of nuclei from one cell to another provides a powerful method to study the interactions of the cytoplasm of one cell with the nuclei of another. Nuclei from various differentiated states can be transferred to nondifferentiated cytoplasm and the effect on the ...
R S, Prather, N L, First
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Nuclear and microtubule reorganization in nuclear‐transferred bovine embryos

Molecular Reproduction and Development, 2002
AbstractWe studied the nuclear and microtubule dynamics in nonactivated and pre‐activated chromatin‐removed oocytes following transfer of nuclei from bovine fibroblast cells. Immediately after fusion between membranes of oocytes and fibroblasts, a microtubule aster containing a γ‐tubulin spot was seen near the transferred nucleus in most oocytes ...
Mi-Ra, Shin, Sang-Wook, Park, Hosup, Shim, Nam-Hyung, Kim   +3 more
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Nuclear Transfer in Nonhuman Primates

2006
The nonhuman primate is a highly relevant model for the study of human diseases, and currently there is a significant need for populations of animals with specific genotypes that can not be satisfied by the capture of animals from the wild or by conventional breeding.
Shoukhrat M, Mitalipov, Don P, Wolf
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Nuclear Transfer in the Mouse Oocyte

2012
The nuclear transfer (NT) technique in the mouse has enabled us to generate cloned mice and to establish NT embryonic stem (ntES) cells. Direct nuclear injection into mouse oocytes with a piezo impact drive unit can aid in the bypass of several steps of the original cell fusion procedure.
Eiji, Mizutani, Atsuo, Ogura, Teruhiko, Wakayama   +2 more
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Epigenetics and nuclear transfer

The Lancet, 2003
Ramiro, Alberio, Keith H S, Campbell
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A method of nuclear transfer

2016
The present invention relates to nuclear transfer methods and embryos developed therefrom. Methods of culturing embryos and reconstituting animals from the embryos generated by the nuclear transfer method of the present invention are also included.
Tecirlioglu, Tayfur, Lewis, Ian, Gabor Vajta   +2 more
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