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Reprogramming in nuclear transfer
Current Opinion in Genetics & Development, 2003Reprogramming a nucleus by transferring it to oocyte cytoplasm triggers epigenetic changes that eventually lead to the restoration of a totipotent state and the birth of a viable animal. A simplified way of studying this complex process is to study cell hybrids.
Jouneau, Alice, Renard, Jean Paul
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Nuclear transfer and reprogramming
Reviews of Reproduction, 1997Nuclear transfer techniques for mammalian embryos have been developed in the last decade. Embryonic nuclei from advanced stages of preimplantation development can be fully reprogrammed and the totipotency is restored when nuclei are transferred into ooplasts. Transfer of nuclei after gene expression from the embryonic genome has started does not appear
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Advances in Livestock Nuclear Transfer
Proceedings of the Society for Experimental Biology and Medicine, 2000Abstract. Cloning and transgenic animal production have been greatly enhanced by the development of nuclear transfer technology. In the past, genetic modification in domestic animals was not tightly controlled. With the nuclear transfer technology one can now create some domestic animals with specific genetic modifications. An ever‐expanding variety of
B, Kühholzer, R S, Prather
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Protocol for Nuclear Transfer in Zebrafish
Cloning and Stem Cells, 2003THE ZEBRAFISH, Danio rerio, has become an important vertebrate model system for extensive studies in various fields of biology. To date, zebrafish has been widely used in studies of genetics, developmental biology, behavior, and human diseases (Dooley and Zon, 2000; Zon, 1999).
Haigen, Huang +3 more
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2019
Nuclear transfer (NT) and cloning offers a unique and powerful experimental tool to study the mechanisms of gene reprogramming, to establish embryonic stem cells from somatic cells, and to clone live offspring. The process of NT involves two different cells.
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Nuclear transfer (NT) and cloning offers a unique and powerful experimental tool to study the mechanisms of gene reprogramming, to establish embryonic stem cells from somatic cells, and to clone live offspring. The process of NT involves two different cells.
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Nuclear transfer and oocyte cryopreservation
Reproduction, Fertility and Development, 2008Somatic cells can be reprogrammed to a totipotent state through nuclear transfer or cloning, because it has been demonstrated that the oocyte has the ability to reprogramme an adult nucleus into an embryonic state that can initiate the development of a new organism.
Ching-Chien, Chang +5 more
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Nuclear transfer into mouse zygotes
Nature Genetics, 2000We have previously reported a novel nuclear-transfer technique to clone mice from adult somatic cells1, 2 and late-passage embryonic stem cells3. The method entails the injection of a somatic-cell nucleus into an enucleated, unfertilized oocyte using a piezo-electrically driven micromanipulator.
T, Wakayama +3 more
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2006
Somatic cell nuclear transfer is a complex and intricate procedure with a low success rate. Despite advances in embryo culture and the production of specialized tools and equipment success rate has remained poor. The procedure remains basically the same despite technical innovations but is now easier to learn and requires less technical expertise ...
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Somatic cell nuclear transfer is a complex and intricate procedure with a low success rate. Despite advances in embryo culture and the production of specialized tools and equipment success rate has remained poor. The procedure remains basically the same despite technical innovations but is now easier to learn and requires less technical expertise ...
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Cloning Zebrafish by Nuclear Transfer
2004Publisher Summary This chapter focuses on cloning zebrafish by nuclear transfer. Cloning of zebrafish by nuclear transfer is a complex procedure involving cell culture, egg selection, and micromanipulation of the eggs and cells, each step affecting the overall efficiency of cloning.
Bensheng, Ju +3 more
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The status of human nuclear transfer
Stem Cell Reviews, 2006Human therapeutic cloning is a recently emerged application of somatic cell nuclear transfer (SCNT), which is currently being performed to produce patient-specific stem cell lines for future stem cell therapies. The advantages in producing human nuclear transfer (NT) embryos to derive NT stem cell lines are that these can be tailor-made (i.e., are ...
Vanessa J, Hall, Miodrag, Stojkovic
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