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Reprogramming Is Essential in Nuclear Transfer
Molecular Reproduction and Development, 2005Fertile offspring have been produced by nuclear transfer from adult somatic cells in several mammalian species (Wilmut et al., 1997; Kato et al., 1998; Wakayama et al., 1998; Polejaeva et al., 2000; Chesne et al., 2002; Shin et al., 2002; Zhou et al., 2003). Various possible causes have been suggested for the overall low efficiency (Perry and Wakayama,
Davor Solter, Takashi Hiiragi
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Cloning and Stem Cells, 2001
The technique of nuclear transfer (NT) allows the production of embryos, fetuses, and offspring from a range of embryonic, fetal, and adult derived cell types in a range of species. Successful development is dependent upon numerous factors, including type of recipient cell, source of recipient cell, method of reconstruction, activation, embryo culture,
K H, Campbell +3 more
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The technique of nuclear transfer (NT) allows the production of embryos, fetuses, and offspring from a range of embryonic, fetal, and adult derived cell types in a range of species. Successful development is dependent upon numerous factors, including type of recipient cell, source of recipient cell, method of reconstruction, activation, embryo culture,
K H, Campbell +3 more
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Quiescence in Nuclear Transfer
Science, 1998When sheep donor cells derived from embryo, fetal, and adult tissues were induced to exit the growth cycle and become quiescent, live lambs were produced after nuclear transfer from such treated cultured cells ([1][1]).
I, Wilmut, K H, Campbell
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2014
Nuclear transfer (NT) technique provides a powerful experimental tool to study the mechanisms of reprogramming processes and to derive NT-embryonic stem (ntES) cells from living or frozen animals. The Piezo-driven direct microinjection NT method has proved to be a valid technique to clone mice and other species.
Brochard, Vincent, Liu, Zichuan
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Nuclear transfer (NT) technique provides a powerful experimental tool to study the mechanisms of reprogramming processes and to derive NT-embryonic stem (ntES) cells from living or frozen animals. The Piezo-driven direct microinjection NT method has proved to be a valid technique to clone mice and other species.
Brochard, Vincent, Liu, Zichuan
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2014
Since 2002, our INRA laboratory (Biologie du Développement et de la Reproduction) has developed a method to produce live somatic clones in rabbit, one of the mammalian species considered as difficult to clone. This chapter presents the technical protocol used nowadays to achieve the goal to obtain cloned embryos able to develop to term using fresh ...
Daniel, Nathalie, Chesné, Patrick
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Since 2002, our INRA laboratory (Biologie du Développement et de la Reproduction) has developed a method to produce live somatic clones in rabbit, one of the mammalian species considered as difficult to clone. This chapter presents the technical protocol used nowadays to achieve the goal to obtain cloned embryos able to develop to term using fresh ...
Daniel, Nathalie, Chesné, Patrick
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Reprogramming in nuclear transfer
Current Opinion in Genetics & Development, 2003Reprogramming a nucleus by transferring it to oocyte cytoplasm triggers epigenetic changes that eventually lead to the restoration of a totipotent state and the birth of a viable animal. A simplified way of studying this complex process is to study cell hybrids.
Jouneau, Alice, Renard, Jean Paul
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Developmental Dynamics, 2006
AbstractDuring development, the genetic content of each cell remains, with a few exceptions, identical to that of the zygote. Differentiated cells, therefore, retain all the genetic information necessary to generate an entire organism (nuclear totipotency).
Alexander, Meissner, Rudolf, Jaenisch
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AbstractDuring development, the genetic content of each cell remains, with a few exceptions, identical to that of the zygote. Differentiated cells, therefore, retain all the genetic information necessary to generate an entire organism (nuclear totipotency).
Alexander, Meissner, Rudolf, Jaenisch
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Nuclear transfer and reprogramming
Reviews of Reproduction, 1997Nuclear transfer techniques for mammalian embryos have been developed in the last decade. Embryonic nuclei from advanced stages of preimplantation development can be fully reprogrammed and the totipotency is restored when nuclei are transferred into ooplasts. Transfer of nuclei after gene expression from the embryonic genome has started does not appear
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Advances in Livestock Nuclear Transfer
Proceedings of the Society for Experimental Biology and Medicine, 2000Abstract. Cloning and transgenic animal production have been greatly enhanced by the development of nuclear transfer technology. In the past, genetic modification in domestic animals was not tightly controlled. With the nuclear transfer technology one can now create some domestic animals with specific genetic modifications. An ever‐expanding variety of
B, Kühholzer, R S, Prather
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2019
Nuclear transfer (NT) and cloning offers a unique and powerful experimental tool to study the mechanisms of gene reprogramming, to establish embryonic stem cells from somatic cells, and to clone live offspring. The process of NT involves two different cells.
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Nuclear transfer (NT) and cloning offers a unique and powerful experimental tool to study the mechanisms of gene reprogramming, to establish embryonic stem cells from somatic cells, and to clone live offspring. The process of NT involves two different cells.
openaire +2 more sources