Results 251 to 260 of about 4,128,907 (296)

Protocol for Nuclear Transfer in Zebrafish

Cloning and Stem Cells, 2003
THE ZEBRAFISH, Danio rerio, has become an important vertebrate model system for extensive studies in various fields of biology. To date, zebrafish has been widely used in studies of genetics, developmental biology, behavior, and human diseases (Dooley and Zon, 2000; Zon, 1999).
Haigen, Huang, Bensheng, Ju, Ki-Young, Lee, Shuo, Lin   +3 more
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Nuclear transfer and oocyte cryopreservation

Reproduction, Fertility and Development, 2008
Somatic cells can be reprogrammed to a totipotent state through nuclear transfer or cloning, because it has been demonstrated that the oocyte has the ability to reprogramme an adult nucleus into an embryonic state that can initiate the development of a new organism.
Ching-Chien, Chang, Li-Ying, Sung, Tomokazu, Amano, X Cindy, Tian, Xiangzhong, Yang, Zsolt Peter, Nagy   +5 more
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Nuclear transfer into mouse zygotes

Nature Genetics, 2000
We have previously reported a novel nuclear-transfer technique to clone mice from adult somatic cells1, 2 and late-passage embryonic stem cells3. The method entails the injection of a somatic-cell nucleus into an enucleated, unfertilized oocyte using a piezo-electrically driven micromanipulator.
T, Wakayama, H, Tateno, P, Mombaerts, R, Yanagimachi   +3 more
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Nuclear Transfer in Sheep

2006
Somatic cell nuclear transfer is a complex and intricate procedure with a low success rate. Despite advances in embryo culture and the production of specialized tools and equipment success rate has remained poor. The procedure remains basically the same despite technical innovations but is now easier to learn and requires less technical expertise ...
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The status of human nuclear transfer

Stem Cell Reviews, 2006
Human therapeutic cloning is a recently emerged application of somatic cell nuclear transfer (SCNT), which is currently being performed to produce patient-specific stem cell lines for future stem cell therapies. The advantages in producing human nuclear transfer (NT) embryos to derive NT stem cell lines are that these can be tailor-made (i.e., are ...
Vanessa J, Hall, Miodrag, Stojkovic
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Nuclear Equivalence, Nuclear Transfer, and the Cell Cycle

Cloning, 1999
The last 20 years have seen the development of techniques for the production of mammals by nuclear transfer. Originally limited to the swapping of pronuclei and the use of early cleavage-stage embryos as nuclear donors, nuclear transfer came of age in 1995 with the birth of 2 Welsh Mountain lambs, Megan and Morag, that were produced using cultured ...
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Cloning Zebrafish by Nuclear Transfer

2004
Publisher Summary This chapter focuses on cloning zebrafish by nuclear transfer. Cloning of zebrafish by nuclear transfer is a complex procedure involving cell culture, egg selection, and micromanipulation of the eggs and cells, each step affecting the overall efficiency of cloning.
Bensheng, Ju, Haigen, Huang, Ki-Young, Lee, Shuo, Lin   +3 more
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Somatic Reprograming by Nuclear Transfer

2020
Somatic cell nuclear transfer (SCNT) is a powerful technique, although challenging, to study reprograming into the totipotent state of differentiated nuclei in mammals. This procedure was initially applied in farm animals, then rodents, and more recently in primates. Nuclear transfer of embryonic stem cells is known to be more efficient, but many types
Brochard, Vincent, Beaujean, Nathalie
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Cloning of Mice by Nuclear Transfer

Cloning and Stem Cells, 2003
ABREAKTHROUGH was made by Wilmut and his colleagues in 1996. A sheep named Dolly was successfully cloned from an adult somatic cell (Wilmut et al., 1997). In this experiment, the donor cell and enucleated oocyte were fused together as the means of nuclear transfer. After that, in 1998, Wakayama et al.
Shaorong, Gao, Michelle, McGarry, Keith E, Latham, Ian, Wilmut   +3 more
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Nuclear Transfer in Mammalian Embryos

1990
Publisher Summary This chapter discusses the nuclear transfer in mammalian embryos. Transfer of nuclei from one cell to another provides a powerful method to study the interactions of the cytoplasm of one cell with the nuclei of another. Nuclei from various differentiated states can be transferred to nondifferentiated cytoplasm and the effect on the ...
R S, Prather, N L, First
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