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Nuclear and microtubule reorganization in nuclear‐transferred bovine embryos
Molecular Reproduction and Development, 2002AbstractWe studied the nuclear and microtubule dynamics in nonactivated and pre‐activated chromatin‐removed oocytes following transfer of nuclei from bovine fibroblast cells. Immediately after fusion between membranes of oocytes and fibroblasts, a microtubule aster containing a γ‐tubulin spot was seen near the transferred nucleus in most oocytes ...
Mi-Ra, Shin +3 more
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Nuclear Transfer and Transgenesis in the Pig
2014Somatic cell nuclear transfer (SCNT) using genetically modified donor cells facilitates the generation of tailored pig models for biomedical research and for xenotransplantation. Up to now, SCNT is the main way to generate gene-targeted pigs, since germ line-competent pluripotent stem cells are not available for this species.
Mayuko, Kurome +4 more
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Nuclear Transfer in Nonhuman Primates
2006The nonhuman primate is a highly relevant model for the study of human diseases, and currently there is a significant need for populations of animals with specific genotypes that can not be satisfied by the capture of animals from the wild or by conventional breeding.
Shoukhrat M, Mitalipov, Don P, Wolf
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Nuclear Transfer in the Mouse Oocyte
2012The nuclear transfer (NT) technique in the mouse has enabled us to generate cloned mice and to establish NT embryonic stem (ntES) cells. Direct nuclear injection into mouse oocytes with a piezo impact drive unit can aid in the bypass of several steps of the original cell fusion procedure.
Eiji, Mizutani +2 more
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Protocols for Nuclear Transfer in Mice
2006Cloning by nuclear transfer in mammals has revealed the remarkable ability of an oocyte to reprogram transferred cell nuclei and induce them to recapitulate the developmental program. This chapter summarizes the method used since 1998 for mouse cloning, which differs from that for large animal cloning.
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Molecular Imaging in Oncology: Current Impact and Future Directions
Ca-A Cancer Journal for Clinicians, 2022Martin G. Pomper, Steven P Rowe
exaly

