Results 151 to 160 of about 528,302 (208)

Studies of inheritance in amoebae by the technique of nuclear transfer

Proceedings of the Royal Society of London. Series B - Biological Sciences, 1958
The methods of classical genetics depend essentially on the reassortment of genes which occurs as a result of meiotic and mating processes. These methods are of great elegance and great power. Upon their success our present theory of inheritance depends. But these methods also have certain weaknesses. These include the following points (1)
J. F. Danielli
openaire   +4 more sources

Simplification of Bovine Somatic Cell Nuclear Transfer by Application of a Zona-Free Manipulation Technique

Cloning and Stem Cells, 2001
Contemporary nuclear transfer techniques often require the involvement of skilled personnel and extended periods of micromanipulation. Here, we present details of the development of a nuclear transfer technique for somatic cells that is both simpler and faster than traditional methods.
Booth, P J, Tan, S J, Reipurth, R, Holm, P, Callesen, H   +4 more
openaire   +6 more sources

Comparison of gene transcription in cloned bovine embryos produced by different nuclear transfer techniques

Molecular Reproduction and Development, 2001
AbstractThe efficiency of animal production using cloning technology is still relatively low and research to determine a more efficient nuclear transfer procedure is ongoing. One approach which may be informative in assessing the viability of nuclear transfer embryos is the analysis of embryonic gene expression.
Robert D. Daniels, Andrew J. French, Natasha A. Korfiatis, Alan O Trounson, Vanessa Jane Hall   +4 more
openaire   +4 more sources

Analysis of the influence of pellet-to-cladding gap on the transient heat transfer in nuclear fuel rods via the integral transform technique [PDF]

Journal of the Brazilian Society of Mechanical Sciences and Engineering, 2018
This work analyzes the transient conduction heat transfer with variable source term in a nuclear reactor fuel rod. Three models are presented to determine the transient temperature distribution across the fuel rod. The results obtained by each modeling are compared in order to evaluate the influence of the gap, as well as the cladding on the thermal ...
Francesco Scofano Neto, Filipe Alcântara Soares, Rodrigo Otávio de Castro Guedes   +2 more
openaire   +3 more sources

A promising nuclear transfer technique for repairing aged oocytes [PDF]

Fertility and Sterility, 2010
S. Awata, Hiroshi Kusunoki, Atsushi Tanaka, Motoi Nagayoshi, Izumi Tanaka, Seiji Watanabe   +5 more
openaire   +3 more sources

Characterization of calcium oscillation patterns in caprine oocytes induced by IVF or an activation technique used in nuclear transfer

Theriogenology, 2006
Routine activation of nuclear transfer (NT) eggs involves the application of a single intracellular calcium [Ca2+]i rise, stimulated by an electrical pulse, as opposed to [Ca2+]i oscillations, which is the natural mode of sperm-induced activation at fertilization in all mammalian species tested to date.
David Melican, S. Nims, Rafael A. Fissore, Carol Ziomek, Robin E. Butler, William G. Gavin, Teru Jellerette   +6 more
openaire   +4 more sources

Assessment of nuclear transfer techniques to prevent the transmission of heritable mitochondrial disorders without compromising embryonic development competence in mice

Mitochondrion, 2014
To evaluate and compare mitochondrial DNA (mtDNA) carry-over and embryonic development potential between different nuclear transfer techniques we performed germinal vesicle nuclear transfer (GV NT), metaphase-II spindle-chromosome-complex (MII-SCC) transfer and pronuclear transfer (PNT) in mice.
Björn Heindryckx, Chen Qian, Rudy Van Coster, Jitesh Neupane, Sabitri Ghimire, Mado Vandewoestyne, Yuechao Lu, Petra De Sutter, Dieter Deforce, Tom Deroo, Jan Gerris   +10 more
openaire   +4 more sources

Microinsemination, nuclear transfer, and cytoplasmic transfer: the application of new reproductive engineering techniques to mouse genetics

Mammalian Genome, 2001
Narumi Ogonuki, Kaoru Takano, Kimiko Inoue, Atsuo Ogura   +3 more
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Rescue of aged oocytes using metaphase-II nuclear transfer with various kinds of fusion techniques [PDF]

Fertility and Sterility, 2013
Seiji Watanabe, Motoi Nagayoshi, Hiroshi Kusunoki, Atsushi Tanaka, Izumi Tanaka   +4 more
openaire   +3 more sources

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Modifications of chemically induced-enucleated nuclear transfer technique by reverse-order nuclear transfer in mouse

Zygote, 2009
SummaryTo improve the developmental potential of somatic cell cloned embryos derived from demecolcine (DC) induced-enucleated nuclear transfer (INT), we modified the INT procedures by transferring donor nuclei into recipient cytoplasts prior to the induced enucleation of the recipient cytoplasts, and we called this modified INT technique as reverse ...
Yong Zhang, Tuo Zhang, Jun Liu, Fengjun Liu, Xiangbin Ding, Zelei Cao, Shuang Tang, Zhilin Guo, Yongsheng Wang, Zhixing An   +9 more
openaire   +3 more sources