Somatic cell nuclear transfer [PDF]
Nature, 2002 Cloning by nuclear transfer from adult somatic cells is a remarkable demonstration of developmental plasticity. When a nucleus is placed in oocyte cytoplasm, the changes in chromatin structure that govern differentiation can be reversed, and the nucleus can be made to control development to term.
Wilmut, I. +7 more
exaly +11 more sources
Generation of genome-edited dogs by somatic cell nuclear transfer [PDF]
BMC Biotechnology, 2022 Background Canine cloning technology based on somatic cell nuclear transfer (SCNT) combined with genome-editing tools such as CRISPR-Cas9 can be used to correct pathogenic mutations in purebred dogs or to generate animal models of disease.
Dong-Ern Kim +6 more
doaj +2 more sources
Derivation of Embryonic Stem Cells from an Endangered Cattle Breed via Somatic Cell Nuclear Transfer [PDF]
CellsEmbryonic stem cells represent a valuable germplasm resource with significant implications for breed conservation, development, and utilization. However, the scarcity of genetic resources in endangered species poses a fundamental constraint on obtaining ...
Shigang Gu +7 more
doaj +2 more sources
Interspecies somatic cell nuclear transfer is dependent on compatible mitochondrial DNA and reprogramming factors. [PDF]
PLoS ONE, 2011 Interspecies somatic cell nuclear transfer (iSCNT) involves the transfer of a nucleus or cell from one species into the cytoplasm of an enucleated oocyte from another.
Yan Jiang +6 more
doaj +9 more sources
Mouse somatic cell nuclear transfer: What has changed and remained unchanged in 25 years [PDF]
The Journal of Reproduction and Development, 2023 Somatic cell nuclear transfer (SCNT) is the only reproductive technology used to produce individuals from somatic cells by transferring them to enucleated oocytes.
Kimiko INOUE
doaj +2 more sources
Oocyte-associated transcription factors in reprogramming after somatic cell nuclear transfer: a review [PDF]
Frontiers of Agricultural Science and Engineering, 2014 Oocytes are unique cells with the inherent capability to reprogram nuclei. The reprogramming of the somatic nucleus from its original cellular state to a totipotent state is essential for term development after somatic cell nuclear transfer.
Fengxia YIN,Hui LIU,Shorgan BOU,Guangpeng LI
doaj +2 more sources
Lessons Learned from Somatic Cell Nuclear Transfer. [PDF]
Int J Mol Sci, 2020 Somatic cell nuclear transfer (SCNT) has been an area of interest in the field of stem cell research and regenerative medicine for the past 20 years. The main biological goal of SCNT is to reverse the differentiated state of a somatic cell, for the purpose of creating blastocysts from which embryonic stem cells (ESCs) can be derived for therapeutic ...
Gouveia C, Huyser C, Egli D, Pepper MS.
europepmc +5 more sources
Reproductive ability of minipigs as surrogates for somatic cell nuclear transfer [PDF]
Journal of Animal Science and TechnologyPigs are genetically, anatomically, and physiologically similar to humans. Recently, pigs are in the spotlight as a suitable source animal for xenotransplantation.
Joonho Moon +5 more
doaj +2 more sources
Chromatin architecture reorganization in murine somatic cell nuclear transfer embryos [PDF]
Nature Communications, 2020 The organisation of chromatin in somatic cell nuclear transfer (SCNT) embryos remains poorly understood. Here, the authors examine higher order chromatin structures of mouse SCNT embryos and provide insights into chromatin architecture reorganisation ...
Mo Chen +13 more
doaj +2 more sources
Lysophosphatidic acid improves development of porcine somatic cell nuclear transfer embryos [PDF]
Journal of Animal Science and TechnologyThis study was conducted to investigate whether lysophosphatidic acid (LPA) could improve the development of porcine somatic cell nuclear transfer (SCNT) embryos.
Ling Sun +5 more
doaj +2 more sources