Results 21 to 30 of about 8,684,016 (282)

The combination of rolipram and cilostamide improved the developmental competence of cloned porcine embryos

Scientific Reports, 2023
In vitro maturation of porcine oocytes is characterized by asynchronous cytoplasmic and nuclear maturation, leading to less competent oocytes supporting embryo development.
Bereket Molla Tanga, Xun Fang, Seonggyu Bang, Chaerim Seo, Heejae Kang, Dabin Cha, Ahmad Yar Qamar, Joohyun Shim, Kimyung Choi, Islam M. Saadeldin, Sanghoon Lee, Jongki Cho   +11 more
doaj   +1 more source

Potential of adipose-derived mesenchymal stem cells and skeletal muscle-derived satellite cells for somatic cell nuclear transfer mediated transgenesis in Arbas Cashmere goats.

PLoS ONE, 2014
Somatic cell nuclear transfer is used to generate genetic models for research and new, genetically modified livestock varieties. Goat fetal fibroblast cells (gFFCs) are the predominant nuclear donors in Cashmere goat transgenic cloning, but have ...
Yu Ren, Haiqing Wu, Yuzhen Ma, Jianlong Yuan, Hao Liang, Dongjun Liu   +5 more
doaj   +1 more source

NUCLEAR REPROGRAMMING AND ITS EPIGENETIC CONSEQUENCES IN SOMATIC CELL NUCLEAR TRANSFER DERIVED ANIMALS [PDF]

SPERMOVA, 2015
Somatic cell nuclear transfer (SCNT) is a powerful tool to understand the mechanisms driving the cellular reprogramming, as well as addressing fundamental questions on developmental biology.
Meirelles FV, Sampaio RV, da Silveira JC, Sangalli JR, Fantinato-Neto P, Birgel Jr. E, Bressan F, Perecin F   +7 more
doaj   +1 more source

Applications of somatic cell nuclear transfer in goats

Journal of Advanced Veterinary and Animal Research, 2014
A number of animals with genetically identical appearance can be produced by somatic cell nuclear transfer (SCNT). From current advancement of SCNT and molecular techniques, production of a transgenic animal becomes easier. Although cloning efficiency in
Ton Yoisungnern, Ashit Kumar Paul
doaj   +1 more source

RNA-Seq Profiling of Intact and Enucleated Oocyte SCNT Embryos Reveals the Role of Pig Oocyte Nucleus in Somatic Reprogramming. [PDF]

PLoS ONE, 2016
The specific molecular mechanisms involved in somatic reprogramming remain unidentified. Removal of the oocyte genome is one of the primary causes of developmental failure in cloned embryos, whereas intact oocyte shows stronger reprogramming capability ...
Lin Bai, Mengqi Li, Junli Sun, Xiaogan Yang, Yangqing Lu, Shengsheng Lu, Kehuan Lu   +6 more
doaj   +1 more source

Comparative Analysis of Nuclear Transfer Embryo Derived Mouse Embryonic Stem Cells. Part I: Cellular characterization [PDF]

, 2012
Embryonic stem cells derived from nuclear transfer embryos (ntESCs) are particularly valuable for regenerative medicine, as they are a patient-specific and histocompatible cell source for the treatment of varying diseases.
Julianna Kobolak, Ruttachuk Rungsiwiwut, Andras Dinnyes, Mamo, Solomon, Ujhelly, Olga, Csonka, Erika, Solomon Mamo, Hadlaczky, Gyula, Olga Ujhelly, Gyula Hadlaczky, Kobolak, Julianna, Dinnyes, Andras, Erika Csonka, Rungsiwiwut, Ruttachuk   +13 more
core   +1 more source

Comparative Analysis of Nuclear Transfer Embryo Derived Mouse Embryonic Stem Cells. Part II: Gene regulation [PDF]

, 2012
In a mouse model nuclear transfer embryo-derived embryonic stem cell lines (ntESCs) of various genetic backgrounds and donor cell types were compared with reference ESCs and analyzed comprehensively at molecular level as a second part of a larger study ...
Julianna Kobolak, Geißler, S., Mamo, S., Horsch, Marion, Horsch, M., Andras Dinnyes, Geissler, Sandra, Mamo, Solomon, Marion Horsch, Kobolak, J., Beckers, Johannes, Solomon Mamo, Kobolak, Julianna, Dinnyes, Andras, Beckers, J., Johannes Beckers, Sandra Geißler, Dinnyes, A.   +17 more
core   +1 more source

Production of porcine reproductive and respiratory syndrome virus (PRRSV)-resistant genomeedited cloned pigs using CRISPR/Cas9n system

浙江大学学报. 农业与生命科学版, 2018
Using gene editing technology based on CRISPR/Cas9n system and somatic cell nuclear transfer technology, we constructed a plasmid of two sgRNAs and Cas9 nickase, and green fluorescent protein (GFP) in the same vector followed by transient transfection of
WANG Shaohua, ZHAO Panpan, LIU Tong, DING Biao, LUO Lei, CAO Zubing, ZHANG Yunhai, ZHANG Kun   +7 more
doaj   +1 more source

Reprogrammed transcriptome in rhesus-bovine interspecies somatic cell nuclear transfer embryos. [PDF]

PLoS ONE, 2011
Global activation of the embryonic genome (EGA), one of the most critical steps in early mammalian embryo development, is recognized as the time when interspecies somatic cell nuclear transfer (iSCNT) embryos fail to thrive.In this study, we analyzed the
Kai Wang, Hasan H Otu, Ying Chen, Young Lee, Keith Latham, Jose B Cibelli   +5 more
doaj   +1 more source

Mitosis gives a brief window of opportunity for a change in gene transcription.

PLoS Biology, 2014
Cell differentiation is remarkably stable but can be reversed by somatic cell nuclear transfer, cell fusion, and iPS. Nuclear transfer to amphibian oocytes provides a special opportunity to test transcriptional reprogramming without cell division.
Richard P Halley-Stott, Jerome Jullien, Vincent Pasque, John Gurdon   +3 more
doaj   +1 more source