Results 31 to 40 of about 110,481 (283)
浙江大学学报. 农业与生命科学版, 2018 Using gene editing technology based on CRISPR/Cas9n system and somatic cell nuclear transfer technology, we constructed a plasmid of two sgRNAs and Cas9 nickase, and green fluorescent protein (GFP) in the same vector followed by transient transfection of
WANG Shaohua +7 more
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Reprogrammed transcriptome in rhesus-bovine interspecies somatic cell nuclear transfer embryos. [PDF]
PLoS ONE, 2011 Global activation of the embryonic genome (EGA), one of the most critical steps in early mammalian embryo development, is recognized as the time when interspecies somatic cell nuclear transfer (iSCNT) embryos fail to thrive.In this study, we analyzed the
Kai Wang +5 more
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Mitosis gives a brief window of opportunity for a change in gene transcription.
PLoS Biology, 2014 Cell differentiation is remarkably stable but can be reversed by somatic cell nuclear transfer, cell fusion, and iPS. Nuclear transfer to amphibian oocytes provides a special opportunity to test transcriptional reprogramming without cell division.
Richard P Halley-Stott +3 more
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Comparative Analysis of Nuclear Transfer Embryo Derived Mouse Embryonic Stem Cells. Part I: Cellular characterization [PDF]
, 2012 Embryonic stem cells derived from nuclear transfer embryos (ntESCs) are particularly valuable for regenerative medicine, as they are a patient-specific and histocompatible cell source for the treatment of varying diseases.
Csonka, Erika +6 more
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BMC Genomics, 2018 Background Nuclear reprogramming reinstates totipotency or pluripotency in somatic cells by changing their gene transcription profile. This technology is widely used in medicine, animal husbandry and other industries.
Yong Liu +13 more
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Asymmetric somatic plant hybridization : status and applications [PDF]
, 2013 To create asymmetric somatic hybrids, the genome of the so-called donor protoplast is fragmented prior to protoplast fusion. As a result, only a limited amount of the donor genome is transferred to the fusion product.
Deryckere, Dieter +4 more
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Generation of bovine transgenics using somatic cell nuclear transfer
Reproductive Biology and Endocrinology, 2003 The ability to produce transgenic animals through the introduction of exogenous DNA has existed for many years. However, past methods available to generate transgenic animals, such as pronuclear microinjection or the use of embryonic stem cells, have ...
Stice Steven L, Hodges Craig A
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Human Somatic Cell Nuclear Transfer Using Adult Cells [PDF]
Cell Stem Cell, 2014 Derivation of patient-specific human pluripotent stem cells via somatic cell nuclear transfer (SCNT) has the potential for applications in a range of therapeutic contexts. However, successful SCNT with human cells has proved challenging to achieve, and thus far has only been reported with fetal or infant somatic cells.
Chung, Young Gie +12 more
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Influences of somatic donor cell sex on and embryo development following somatic cell nuclear transfer in pigs [PDF]
Asian-Australasian Journal of Animal Sciences, 2017 Objective The present study investigates pre- and post-implantation developmental competence of nuclear-transferred porcine embryos derived from male and female fetal fibroblasts.
Jae-Gyu Yoo +9 more
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Mouse Ooplasm Confers Context-Specific Reprogramming Capacity [PDF]
, 2014 Enucleated oocytes have the remarkable ability to reprogram somatic nuclei back to totipotency. Here we investigate genome-scale DNA methylation patterns after nuclear transfer and compare them to the dynamics at fertilization.
Chan, Michelle +4 more
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