Results 31 to 40 of about 42,166 (286)

Comparative Analysis of Nuclear Transfer Embryo Derived Mouse Embryonic Stem Cells. Part II: Gene regulation [PDF]

, 2012
In a mouse model nuclear transfer embryo-derived embryonic stem cell lines (ntESCs) of various genetic backgrounds and donor cell types were compared with reference ESCs and analyzed comprehensively at molecular level as a second part of a larger study ...
Julianna Kobolak, Geißler, S., Mamo, S., Horsch, Marion, Horsch, M., Andras Dinnyes, Geissler, Sandra, Mamo, Solomon, Marion Horsch, Kobolak, J., Beckers, Johannes, Solomon Mamo, Kobolak, Julianna, Dinnyes, Andras, Beckers, J., Johannes Beckers, Sandra Geißler, Dinnyes, A.   +17 more
core   +1 more source

Applications of somatic cell nuclear transfer in goats

Journal of Advanced Veterinary and Animal Research, 2014
A number of animals with genetically identical appearance can be produced by somatic cell nuclear transfer (SCNT). From current advancement of SCNT and molecular techniques, production of a transgenic animal becomes easier. Although cloning efficiency in
Ton Yoisungnern, Ashit Kumar Paul
doaj   +1 more source

Factors influencing the somatic cell nuclear transfer efficiency in pigs [PDF]

Frontiers of Agricultural Science and Engineering, 2019
Using a data set from our laboratory, we assessed the effects of several factors on pig cloning efficiency. The results demonstrated that cells at high confluence (>90%) used as donor cell resulted in higher pregnancy rate, delivery rate and overall ...
Yong JIN, Manling ZHANG, Xinrong JU, Shuang LIANG, Qiang XIONG, Lihua ZHAO, Xiaowei NIE, Daorong HOU, Qiang LIU, Junzheng WANG, Chenyu WANG, Xiaokang LI, Lining ZHANG, Xiaorui LIU, Ying WANG, Haiyuan YANG, Yifan DAI, Rongfeng LI
doaj   +1 more source

Mitosis gives a brief window of opportunity for a change in gene transcription.

PLoS Biology, 2014
Cell differentiation is remarkably stable but can be reversed by somatic cell nuclear transfer, cell fusion, and iPS. Nuclear transfer to amphibian oocytes provides a special opportunity to test transcriptional reprogramming without cell division.
Richard P Halley-Stott, Jerome Jullien, Vincent Pasque, John Gurdon   +3 more
doaj   +1 more source

Production of porcine reproductive and respiratory syndrome virus (PRRSV)-resistant genomeedited cloned pigs using CRISPR/Cas9n system

浙江大学学报. 农业与生命科学版, 2018
Using gene editing technology based on CRISPR/Cas9n system and somatic cell nuclear transfer technology, we constructed a plasmid of two sgRNAs and Cas9 nickase, and green fluorescent protein (GFP) in the same vector followed by transient transfection of
WANG Shaohua, ZHAO Panpan, LIU Tong, DING Biao, LUO Lei, CAO Zubing, ZHANG Yunhai, ZHANG Kun   +7 more
doaj   +1 more source

Reprogrammed transcriptome in rhesus-bovine interspecies somatic cell nuclear transfer embryos. [PDF]

PLoS ONE, 2011
Global activation of the embryonic genome (EGA), one of the most critical steps in early mammalian embryo development, is recognized as the time when interspecies somatic cell nuclear transfer (iSCNT) embryos fail to thrive.In this study, we analyzed the
Kai Wang, Hasan H Otu, Ying Chen, Young Lee, Keith Latham, Jose B Cibelli   +5 more
doaj   +1 more source

Somatic Cell Nuclear Transfer Using Freeze-Dried Protaminized Donor Nuclei

, 2023
Somatic cell nuclear transfer (SCNT) is the only nuclear reprogramming method that allows rewinding an adult nucleus into a totipotent state. As such, it offers excellent opportunities for the multiplication of elite genotypes or endangered animals ...
Loi, Pasqualino, Matsukawa, Kazutsugu, Palazzese, Luca, Czernik, Marta   +3 more
core   +1 more source

Transcriptional defects and reprogramming barriers in somatic cell nuclear reprogramming as revealed by single-embryo RNA sequencing

BMC Genomics, 2018
Background Nuclear reprogramming reinstates totipotency or pluripotency in somatic cells by changing their gene transcription profile. This technology is widely used in medicine, animal husbandry and other industries.
Yong Liu, Fengrui Wu, Ling Zhang, Xiaoqing Wu, Dengkun Li, Jing Xin, Juan Xie, Feng Kong, Wenying Wang, Qiaoqin Wu, Di Zhang, Rong Wang, Shaorong Gao, Wenyong Li   +13 more
doaj   +1 more source

Influences of somatic donor cell sex on and embryo development following somatic cell nuclear transfer in pigs [PDF]

Asian-Australasian Journal of Animal Sciences, 2017
Objective The present study investigates pre- and post-implantation developmental competence of nuclear-transferred porcine embryos derived from male and female fetal fibroblasts.
Jae-Gyu Yoo, Byeong-Woo Kim, Mi-Rung Park, Deug-Nam Kwon, Yun-Jung Choi, Teak-Soon Shin, Byung-Wook Cho, Jakyeom Seo, Jin-Hoi Kim, Seong-Keun Cho   +9 more
doaj   +1 more source

Epigenetic manipulation to improve mouse SCNT embryonic development

Frontiers in Genetics, 2022
Cloned mammals can be achieved through somatic cell nuclear transfer (SCNT), which involves reprogramming of differentiated somatic cells into a totipotent state. However, low cloning efficiency hampers its application severely.
Yamei Li, Yamei Li, Yamei Li, Qiang Sun, Qiang Sun, Qiang Sun   +5 more
doaj   +1 more source