Results 81 to 90 of about 6,071,343 (411)

Site-Specific Modification Using the 2′-Methoxyethyl Group Improves the Specificity and Activity of siRNAs

Molecular Therapy: Nucleic Acids, 2017
Rapid progress has been made toward small interfering RNA (siRNA)-based therapy for human disorders, but rationally optimizing siRNAs for high specificity and potent silencing remains a challenge.
Xinyun Song, Xiaoxia Wang, Yuan Ma, Zicai Liang, Zhenjun Yang, Huiqing Cao   +5 more
doaj   +1 more source

Functional DNA Regulated CRISPR-Cas12a Sensors for Point-of-Care Diagnostics of Non-Nucleic Acid Targets.

Journal of the American Chemical Society, 2019
Beyond its extraordinary genome editing ability, the CRISPR-Cas system has opened a new era of biosensing applications due to its high base resolution and isothermal signal amplification. However, the reported CRISPR-Cas sensors are largely only used for
Ying Xiong, Jingjing Zhang, Zhenglin Yang, Quanbing Mou, Yuan Ma, Yonghua Xiong, Yi Lu   +6 more
semanticscholar   +1 more source

Subpar reporting of pre‐analytical variables in RNA‐focused blood plasma studies

Molecular Oncology, EarlyView.
Pre‐analytical variables strongly influence the analysis of extracellular RNA (cell‐free RNA; exRNA) derived from blood plasma. Their reporting is essential to allow interpretation and replication of results. By evaluating 200 exRNA studies, we pinpoint a lack of reporting pre‐analytical variables associated with blood collection, plasma preparation ...
Céleste Van Der Schueren, Philippe Decruyenaere, Francisco Avila Cobos, Johanna Bult, Jill Deleu, Laudonia Lidia Dipalo, Hetty Hilde Helsmoortel, Eva Hulstaert, Annelien Morlion, Elena Ramos Varas, Kathleen Schoofs, Wim Trypsteen, Eveline Vanden Eynde, Hanne Van Droogenbroeck, Kimberly Verniers, Jo Vandesompele, Anneleen Decock   +16 more
wiley   +1 more source

Visualizing germination of microbiota endospores in the mammalian gut

Gut Microbes, 2022
Transmission of bacterial endospores between the environment and people and the following germination in vivo play critical roles in both the deadly infections of some bacterial pathogens and the stabilization of the commensal microbiotas in humans.
Ningning Xu, Liyuan Lin, Yahui Du, Huibin Lin, Jia Song, Chaoyong Yang, Wei Wang   +6 more
doaj   +1 more source

The Effects of Stacking on the Configurations and Elasticity of Single Stranded Nucleic Acids [PDF]

Phys. Rev. E 70 020902(R) (2004), 2004
Stacking interactions in single stranded nucleic acids give rise to configurations of an annealed rod-coil multiblock copolymer. Theoretical analysis identifies the resulting signatures for long homopolynucleotides: A non monotonous dependence of size on temperature, corresponding effects on cyclization and a plateau in the extension force law ...
arxiv   +1 more source

Rosetta Brains: A Strategy for Molecularly-Annotated Connectomics [PDF]

, 2014
We propose a neural connectomics strategy called Fluorescent In-Situ Sequencing of Barcoded Individual Neuronal Connections (FISSEQ-BOINC), leveraging fluorescent in situ nucleic acid sequencing in fixed tissue (FISSEQ).
Boyden, Edward S, Church, George M, Daugharthy, Evan R, Kalhor, Reza, Kebschull, Justus M, Kording, Konrad P, Lee, Je Hyuk, Marblestone, Adam H, Mishchenko, Yuriy, Peikon, Ian D, Shipman, Seth L, Zador, Anthony M   +11 more
core  

Polyphenolic C-glucosidic ellagitannins present in oak-aged wine inhibit HIV-1 nucleocapsid protein [PDF]

, 2015
HIV-1 nucleocapsid protein (NC) is a nucleic acid chaperone implicated in several steps of the virus replication cycle and an attractive new target for drug development.
Cappellini, Marta, Deffieux, Denis, Fabris, Dan, Gatto, Barbara, Jacquet, R\ue9mi, Quideau, St\ue9phane, Sinigaglia, Laura, Sosic, Alice   +7 more
core   +1 more source

CRISPR-Cas12a-assisted nucleic acid detection

Cell Discovery, 2018
Dear Editor, Today, the need for time-effective and cost-effective nucleic acid detection methods is still growing in fields such as human genotyping and pathogen detection.
Shiyuan Li, Qiu-Xiang Cheng, Jing-Man Wang, Xiao-Yan Li, Zi-Long Zhang, Song Gao, Rui-Bing Cao, Guoping Zhao, Jin Wang   +8 more
semanticscholar   +1 more source

Clinical applications of next‐generation sequencing‐based ctDNA analyses in breast cancer: defining treatment targets and dynamic changes during disease progression

Molecular Oncology, EarlyView.
Circulating tumor DNA (ctDNA) offers a possibility for different applications in early and late stage breast cancer management. In early breast cancer tumor informed approaches are increasingly used for detecting molecular residual disease (MRD) and early recurrence. In advanced stage, ctDNA provides a possibility for monitoring disease progression and
Eva Valentina Klocker, Samantha Hasenleithner, Rupert Bartsch, Simon P. Gampenrieder, Daniel Egle, Christian F. Singer, Gabriel Rinnerthaler, Michael Hubalek, Katja Schmitz, Zsuzsanna Bago‐Horvath, Andreas Petzer, Sonja Heibl, Ellen Heitzer, Marija Balic, Michael Gnant   +14 more
wiley   +1 more source

Integrated identification of growth pattern and taxon of bacterium in gut microbiota via confocal fluorescence imaging‐oriented single‐cell sequencing

mLife, 2022
Despite the fast progress in our understanding of the complex functions of gut microbiota, it is still challenging to directly investigate the in vivo microbial activities and processes on an individual cell basis.
Juan Gao, Di Sun, Bei Li, Chaoyong Yang, Wei Wang   +4 more
doaj   +1 more source