Results 111 to 120 of about 210,426 (263)

Thermostable Bioluminescent Intercalating Dyes for Real-Time, Integrated Nucleic Acid Amplification and Detection. [PDF]

Angew Chem Int Ed Engl
de Stigter Y, van der Veer HJ, de Lignie S, de Haas RJ, de Vries R, Schoeber JPH, Loonen AJM, van den Brule AJC, Merkx M.   +8 more
europepmc   +1 more source

Conditionally Significant eQTL and TWAS Analyses Identify MsRD26 as a Major Candidate Positive Regulator of Salt Tolerance in Medicago sativa L

Advanced Science, EarlyView.
Salt stress significantly impacts plant growth. Through population transcriptome, eQTL and TWAS analyses, we identified several candidate genes potentially associated with salt stress response. Functional assays suggested that some of these genes may contribute to salt tolerance.
Lin Chen, Yuqi Zhang, Xinyue Ma, Jinpeng Bi, Fei He, Li Zhao, Haiyue Lei, Zhengqin Xiao, Xue Wang, Tiejun Zhang, Ruicai Long, Junmei Kang, Qingchuan Yang   +12 more
wiley   +1 more source

RNA Promotes Synapsin Condensates That Organize Synaptic Vesicles in Live Neurons and Enable Localized Translation in Reconstituted System

Advanced Science, EarlyView.
Condensates at synapses organize synaptic vesicles (SVs) and are crucial for efficient neurotransmitter release, yet how RNA contributes to this mesoscale architecture remains poorly understood. Here we uncover that RNA modulates synapsin–driven condensates.
Branislava Rankovic, Zachary M. Geisterfer, Akshita Chhabra, Vladimir M. Jovanovic, Ian Seim, Christian Hoffmann, Antonela Condric, Gerard Aguilar Pérez, Rinse de Boer, Lars T. Hofstede, Jingao Sun, Kiara Freitag, Katja Nowick, Marina Jendrach, Frank L. Heppner, Vladimir Despic, Michaela Müller‐McNicoll, Stephan Sigrist, Geert van den Bogaart, Sofiia Reshetniak, Amy S. Gladfelter, Dragomir Milovanovic   +21 more
wiley   +1 more source

Before Dengue Is Implicated as the Cause of Polyradiculitis, the Infection Must Be Confirmed by Nucleic Acid Amplification Testing or Cell Cultures. [PDF]

Ann Neurosci
Finsterer J.
europepmc   +1 more source

A Multidimensional Engineering Strategy Reprograms Microglia via Targeted and Sustained‐Release Extracellular Vesicles for Spinal Cord Injury Repair

Advanced Science, EarlyView.
A multifunctional EV‐based nanoplatform (Ang‐TEVs@Gel) was engineered via preconditioning, surface targeting, and ROS‐responsive hydrogel encapsulation to reprogram microglia. This system restored autophagy via miR‐664a‐3p/PIK3CA axis, cleared myelin debris, resolved neuroinflammation, and promoted axon remyelination, ultimately achieving robust motor ...
Wu Xiong, Minhao Liu, Mingming Zheng, Jizhou Jia, Ziyan Zhu, Guang Kong, Jie Liu, Juan Wang, Wenbo Li, Qingyuan Wang, Qian Zhu, Peiran Chan, Tao Qin, Jiayang Wu, Yongjie Zhang, Chunming Tang, Cong Li, Jin Fan   +17 more
wiley   +1 more source

Light-Activated Nucleic Acid Amplification Systems Using Photo-Caged DNA Polymerase or Primers. [PDF]

Adv Genet (Hoboken)
Wu Y, Wu M, Tian Y, Chen H, Sun X, Ma C, Liu J, Xia F, Liu Y, Pei X, Wang J, Zhao G, Wang N, Wang F, Liu Q, Yu F, Fan X, Wang Z, Ermilova E, West JS, Cheng Q.   +20 more
europepmc   +1 more source

Dual‐line Genome‐scale CRISPR Screening Enables Robust Target Gene Discovery

Advanced Science, EarlyView.
A species‐optimized CRISPR platform integrates efficient piggyBac delivery, genome‐scale sgRNA libraries, and parallel screening in two independently engineered Bactrocera dorsalis Cas9 cell lines. Cross‐line consensus analysis filters line‐specific effects, enriches candidates with reproducible in vivo phenotypes, and reveals conserved, species ...
Ziniu Li, Jiao Qiao, Qiuyuan Zhang, Peipei Liu, Chenjun Zheng, Qi Zhou, Mengqi Ji, Zhihui Zhu, Xiaoxue Li, Hongyu Zhang   +9 more
wiley   +1 more source

Establishment of Nucleic Acid Amplification Technology for the Detection of <i>Mycoplasma</i> in Biological Products. [PDF]

Molecules
Guo Y, Qin X, Zhang J, Bi H, Hou S, Ding Y, Pei D, Li X, Pan Y, Sun X, Liang C.   +10 more
europepmc   +1 more source

Controllable Cascade Aggregation Ion Programmed Nanomachines and Simple Isolation Enable Tumor‐Derived Small Extracellular Vesicles Detection for Enhanced Breast Cancer Assessment

Advanced Science, EarlyView.
This study developed a convenient, enzyme‐free detection platform based on controllable aggregation ion nanomachines. Its favorable anti‐interference capacity and high sensitivity meet the detection requirements for clinical samples processed via a simple filtration method within 10 min.
Zhihao Zhang, Zijing Liu, Xiangyue Meng, Pengjun Jiang, Jie Qu, Yu Jiang, Binxu Qiu, Jie Chen, Piaopiao Chen   +8 more
wiley   +1 more source

Nucleic Acid Amplification Tests for <i>Candida</i> Species Identification: A Systematic Review of Diagnostic Performance. [PDF]

Pathogens
Czajka KM, Bilgasem A, Al-Jumaili YA, Kitching D, Buchan G, Nair A, Reich M, Ijomah C, Saikrishna GE, Verschoor C, Santi SA, Brabant-Kirwan D, Singh R, Appanna V, Saunders D, Tharmalingam S.   +15 more
europepmc   +1 more source