Results 291 to 300 of about 186,936 (344)

Biosensors based on functional nucleic acids and isothermal amplification techniques

Talanta, 2023
In the past few years, with the in-depth research of functional nucleic acids and isothermal amplification techniques, their applications in the field of biosensing have attracted great interest. Since functional nucleic acids have excellent flexibility and convenience in their structural design, they have significant advantages as recognition elements
Xiuen Cao, Chuanpin Chen, Qubo Zhu
openaire   +2 more sources

Hairpin DNA-Mediated isothermal amplification (HDMIA) techniques for nucleic acid testing

Talanta, 2021
Nucleic acid detection is of great importance in a variety of areas, from life science and clinical diagnosis to environmental monitoring and food safety. Unfortunately, nucleic acid targets are always found in trace amounts and their response signals are difficult to be detected. Amplification mechanisms are then practically needed to either duplicate
Idorenyin A. Iwe, Wenqin Li, Zhigang Li, Jiahao Huang   +3 more
openaire   +3 more sources

A netlike rolling circle nucleic acid amplification technique

The Analyst, 2015
An isothermal nucleic acid amplification technique termed as netlike rolling circle amplification is proposed. Dense and uniform network morphology of amplified products is first observed, suggesting the ultrahigh amplification efficiency.
Xiaoli, Zhu, Chang, Feng, Bin, Zhang, Hui, Tong, Tao, Gao, Genxi, Li   +5 more
openaire   +2 more sources

Nucleic Acid Amplification Techniques in Immunoassay: An Integrated Approach with Hybrid Performance

Journal of Agricultural and Food Chemistry, 2021
An immunoassay is mostly employed for the direct detection of food contaminants, and a molecular assay for targeting nucleic acids employs amplification techniques for distinguishing genes. The integration of an immunoassay with nucleic acid amplification techniques inherits the direct and rapid performance of an immunoassay and the ultrasensitive ...
Panting Sang, Zhigang Hu, Yuliang Cheng, Hang Yu, Yunfei Xie, Weirong Yao, Yahui Guo, He Qian   +7 more
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Bioanalytical applications of isothermal nucleic acid amplification techniques

Analytica Chimica Acta, 2015
The most popular in vitro nucleic acid amplification techniques like polymerase chain reaction (PCR) including real-time PCR are costly and require thermocycling, rendering them unsuitable for uses at point-of-care. Highly efficient in vitro nucleic acid amplification techniques using simple, portable and low-cost instruments are crucial in disease ...
Huimin Deng, Zhiqiang Gao
openaire   +2 more sources

New nucleic acid amplification techniques in diagnostic pathology

Journal of the European Academy of Dermatology and Venereology, 1994
AbstractIn 1985, Mullis discovered an exponentially increasing amplification system for nucleic acids, which became known as the polymerase chain reaction (PCR). This method permits the selective in vitro amplification of a particular DNA region by mimicking phenomena of in vivo DNA replication.
J.J. O'Leary, G. Browne, R. Landers, M. Crowley, I. Healy, M.I. Johnson, C.T. Doyle   +6 more
openaire   +1 more source

Validation criteria for nucleic acid amplification techniques for bacterial infections

Clinical Chemistry and Laboratory Medicine, 2008
Nucleic acid techniques (NATs), such as species-specific and universal polymerase chain reactions (PCRs), are finding ever wider use in the diagnosis of bacterial infection. However, although universal PCR assays, in particular, approach a type of modern Petri dish, they have a number of limitations which restrict their applicability.
Gerd, Döring, Klaus, Unertl, Alexandra, Heininger   +2 more
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Nucleic acid amplification and sequencing techniques

1993
While nucleic acid hybridization procedures have now developed to the extent that they can be used readily for identification and typing of a wide range of microorganisms, the target nucleic acid is available only in very limited quantities unless the organism is first cultivated. Culture methods have not, however, been devised for some microorganisms,
K. J. Towner, A. Cockayne
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Quantitative Reverse Transcription Strand Displacement Amplification: Quantitation of Nucleic Acids Using an Isothermal Amplification Technique

Analytical Biochemistry, 1998
Recent advances in nucleic acid amplification techniques have allowed for quantitation of viral nucleic acid levels in clinical specimens. The most prevalent testing is carried out for HIV viral load. Strand displacement amplification (SDA) is an isothermal DNA amplification system utilizing a restriction enzyme and a DNA polymerase with strand ...
C M, Nycz, C H, Dean, P D, Haaland, C A, Spargo, G T, Walker   +4 more
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Hairpin DNA-mediated isothermal amplification techniques for nucleic acid testing

2021
Nucleic acid detection is of great importance in a variety of areas, from life science and clinical diagnosis to environmental monitoring and food safety. Unfortunately, nucleic acid targets are always found in trace amounts and their response signals are difficult to be detected. Amplification mechanisms are then practically needed to either duplicate
openaire   +2 more sources