Results 91 to 100 of about 3,983,345 (293)

[dataset] Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits

, 2019
Full dataset for "Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits" published in Scientific ...
Erik Jue
core   +1 more source

Comparison of DNA extraction protocols for Mycobacterium Tuberculosis in diagnosis of tuberculous meningitis by real-time polymerase chain reaction

Journal of Global Infectious Diseases, 2011
Background: Several nucleic acid amplification techniques are available for detection of Mycobacterium tuberculosis (MTB) in pulmonary and extrapulmonary samples, but insufficient data are available on the diagnostic utility of these techniques in ...
Rajeev Thakur, Smita Sarma, Renu Goyal
doaj   +1 more source

Additional file 3 of Digital PCR-based evaluation of nucleic acid extraction kit performance for the co-purification of cell-free DNA and RNA

, 2022
Additional file 3: Table S2. Comparison of expected and measured cfDNA or cfRNA concentration ratio, based on plasma input volume, within a given nucleic acid extraction ...
Angie Denolf (15239300), Kathleen Schoofs (15239291), Jill Deleu (5743235), Anneleen Decock (1263777), Tom Van Maerken (105035), Katleen De Preter (795), Joke Verreth (15239303), Kimberly Verniers (15239294), Jo Vandesompele (794), Bram De Wilde (180078), Sofie Roelandt (15239297)   +10 more
core   +1 more source

Evaluation of Dried Plasma Spot‐Based Quantification of Glial Fibrillary Acidic Protein as a Disease‐Associated Biomarker in Neuromyelitis Optica Spectrum Disorder

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To evaluate the diagnostic accuracy of glial fibrillary acidic protein (GFAP) measured in dried plasma spots versus conventional plasma‐ and serum‐GFAP testing for assessment of disease severity in aquaporin‐4 immunoglobulin G–positive neuromyelitis optica spectrum disorder (AQP4‐IgG+ NMOSD).
Felix Wohlrab, Evelyn Alvarez, Roua Hamdi, Bingqing Zhang, Hayeun Ji, Niyati Jhaveri, Patrick Schindler, Pedro Sanchez, Pia S. Sperber, Tanja Schmitz‐Hübsch, Frederike C. Oertel, Nisa Vorasoot, Najib Kissani, Falko Böhringer, Jens Kuhle, Anne‐Katrin Pröbstel, Xiao‐Jun Ma, Péter Körtvelyessy, Patrick Waters, Friedemann Paul   +19 more
wiley   +1 more source

Melanin's Role in Balancing Mechanical Strength and Reproductive Output in Environmentally Stressed Fungal Spores

Advanced Functional Materials, EarlyView.
Melanin content directly controls fungal spore mechanical properties, with pigmented spores achieving rupture forces two orders of magnitude higher than hyaline spores. Environmental stress drives this quantity‐quality tradeoff; stressed species invest energy in melanin‐reinforced, mechanically superior thick‐walled, super‐hydrophobic spores, while ...
Atul Agrawal, Steven E. Naleway
wiley   +1 more source

Optimized approaches to profile low biomass DNA and RNA virome in the upper respiratory tract of beef cattle

Animal Microbiome
Background Viruses play a key role in cattle respiratory tract (RT) health, yet, profiling the upper respiratory tract (URT) virome (DNA and RNA viruses) from deep nasopharyngeal swabs (DNS) remains challenging due to their low biomass, lack of well ...
Yue Wang, Mi Zhou, Nilusha Malmuthuge, Yanhong Chen, Trevor W. Alexander, Edouard Timsit, Le Luo Guan   +6 more
doaj   +1 more source

Development of an Optimized Two-Step Solid-Phase Extraction Method for Urinary Nucleic Acid Adductomics

Biomolecules
The exposome represents the totality of endogenous and exogenous exposures across the lifespan. These exposures may result in DNA and RNA damage, in the form of adducts, which is a key factor in the etiology of a variety of human diseases, including ...
Alexandra Keidel, Jazmine Virzi, Laura Deloso, Carolina Möller, Dale Chaput, Theresa Evans-Nguyen, Yuan-Jhe Chang, Mu-Rong Chao, Chiung-Wen Hu, Marcus S. Cooke   +9 more
doaj   +1 more source

ATP‐Mediated Bidirectional Communication Between Natural and Artificial Cells for Controlled Bioreactions

Advanced Functional Materials, EarlyView.
Establishment of bidirectional communication behavior between natural and artificial cells. The first type of communication from natural dendritic cells to artificial polymeric cells was achieved via ATP release after cell lysis. The released ATP was taken up via gated UV‐controlled permeability increase of the polymeric membrane for performing ...
Ilona Kalte, Tsvetomir Ivanov, Leonhard Seufert, Shoupeng Cao, Lucas Caire da Silva, Volker Mailänder, Katharina Landfester   +6 more
wiley   +1 more source

A Holistic Platform Approach for Developing Robust Pulmonary mRNA Delivery: Integrating Novel Phenolic Acid‐Derived Ionizable Lipids and Device Engineering

Advanced Functional Materials, EarlyView.
An integrated pulmonary mRNA delivery platform combining novel biodegradable syringic acid‐derived ionizable lipids, design‐of‐experiments formulation optimization, and vibrating‐mesh nebulizer engineering enabled stable aerosolization and efficient lung delivery.
Neha Kaushal, Younan Ma, Asad Khanmohammed, Dipen Parande, Hanming Zhang, Saket Dolare, Juliet Chen, Apiwat Wangweerawong, Saswata Karmakar, Hongfeng Deng, Frank Koppenhagen, Shraddha Sharma, Eric Reyes, Sherry He, Lianne Boeglin, Caroline Woo, Azadeh Paksa, Ayed Allawzi, Anusha Dias, Gregory Troiano, Shrirang Karve, Frank DeRosa   +21 more
wiley   +1 more source

Advances in Nucleic Acid Universal Influenza Vaccines

Currently, vaccination with influenza vaccines is still an effective strategy to prevent infection by seasonal influenza virus in spite of some drawbacks with them.
Weigang Ren, Qin Wang, Junwei Li, Liang Xu   +3 more
core   +1 more source