Results 81 to 90 of about 3,983,345 (293)

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

Cell Nucleus Targeting for Living Cell Extraction of Nucleic Acid Associated Proteins with Intracellular Nanoprobes of Magnetic Carbon Nanotubes

, 2013
Since nanoparticles could be ingested by cells naturally and target at a specific cellular location as designed, the extraction of intracellular proteins from living cells for large-scale analysis by nanoprobes seems to be ideally possible.
Qin, Hongqiang, Hu, Zhengyan, Zou, Hanfa, 邹汉法, 吴仁安, Zhang, Yi, Liu, Fangjie, Wu, Ren'an, Cheng, Kai   +8 more
core   +1 more source

Ultrafast visual detection of Streptococcus suis via HtpsC‐targeted LAMP–CRISPR–Cas12a coupled with lateral flow immunochromatography

FEBS Open Bio, EarlyView.
A rapid visual test combines DNA amplification, CRISPR–based target verification, and a lateral flow strip to detect Streptococcus suis. The assay gives results in about 45 min, reduces false‐positive interpretation, and does not require fluorescence equipment.
Liu‐xin Li, Miao Xin, Xiao‐wen Cui, Shi‐yin Yu, Fei Meng, Min Cao, Xiao‐hui Xiong, Yi‐chen Lu   +7 more
wiley   +1 more source

Integrated High-Throughput Centrifugal Microfluidic Chip Device for Pathogen Detection On-Site

Biosensors
An integrated and high-throughput device for pathogen detection is crucial in point-of-care testing (POCT), especially for early diagnosis of infectious diseases and preventing the spread of infection.
Shuyu Lu, Yuanzhan Yang, Siqi Cui, Anyi Li, Cheng Qian, Xiaoqiong Li   +5 more
doaj   +1 more source

Nucleic acid extraction from biomining microorganisms

, 2009
Various methods of nucleic acid (NA) extraction were investigated with the aim of developing a quantitative method of NA extraction from five representative strains of biomining microorganisms.
Elizabeth Watkin (12315074), Carla Zammit (12727532), Lesley Mutch (23319979), H.R. Watling (23324569)   +3 more
core   +2 more sources

Plasma EV Proteomics Identifies ECM Remodeling and Inflammatory Proteins LUM and C7 as Candidate Biomarkers in FSHD

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Facioscapulohumeral muscular dystrophy (FSHD) is one of the most debilitating and common muscular dystrophies. Despite its severity, no approved therapy exists for FSHD patients. However, several therapeutic candidates are currently under development, and some have recently entered clinical trials, marking the need for reliable ...
Mustafa Bilal Bayazit, Chiranth K. Nagaraj, Jackson S. Newell, Kim Truc Nguyen, Xilal Y. Rima, Jacob Doon‐Ralls, Eduardo Reátegui, Jeffrey M. Statland, Rabi Tawil, Kevin M. Flanigan, Scott Q. Harper, Nizar Y. Saad   +11 more
wiley   +1 more source

Manual Nucleic Acid Extraction using MagMAX™ CORE Nucleic Acid Purification Kit for Whole Blood and Cell Supernatant v1

This protocol describes a standardized, manual workflow for extracting nucleic acids (DNA/RNA) using the Applied Biosystems‱ MagMAX‱ CORE Nucleic Acid Purification Kit on a magnetic bead-based particle processor.
Jennifer Welch, Suelee Robbe-Austerman, Lindsey Gabbert, Rachel Palinski, Robin Holland   +4 more
core   +1 more source

Validating an Automated Nucleic Acid Extraction Device for Omics in Space Using Whole Cell Microbial Reference Standards

Frontiers in Microbiology, 2020
NASA has made great strides in the past five years to develop a suite of instruments for the International Space Station in order to perform molecular biology in space.
Camilla Urbaniak, Season Wong, Scott Tighe, Arunkumar Arumugam, Bo Liu, Ceth W. Parker, Jason M. Wood, Nitin K. Singh, Dana J. Skorupa, Brent M. Peyton, Ryan Jenson, Fathi Karouia, Julie Dragon, Kasthuri Venkateswaran   +13 more
doaj   +1 more source

Disposable, pressure-driven, and self-contained cartridge with pre-stored reagents for automated nucleic acid extraction

, 2023
Nucleic acid extraction is vital in many applications such as molecular diagnostics, genetic engineering, and deoxyribonucleic acid (DNA) sequencing.
Shin, Joong Ho, Shin, Joong Ho,, Thang, Le Tran Huy, Shin, Jae Ho, Shin, Jae Ho,, Thang, Le Tran Huy,, Han, Won, Han, Won,   +7 more
core   +1 more source

Blood RNA Biomarker Signatures for Early Diagnosis and Prognosis in Ischemic and Hemorrhagic Stroke: The IBIS‐CT1 Study

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To evaluate the expression of nine blood RNA biomarkers in a clinical trial based on genes previously identified in an experimental monkey model of stroke for diagnosis feasibility and prognostication. Methods IBIS‐CT1 was a prospective longitudinal study enrolling patients with ischemic stroke (IS) or intracerebral hemorrhage (ICH ...
Salomé Retailleau, Marie Bruguet, Irina Viakhireva‐Dovganyuk, François‐Mathias Merrien, Denis Marechal, Aurore Jourdain, Philippe Goas, Jordan Coris, Julien Asselineau, Maëlys Consigny, Gérald Le Gac, Serge Timsit   +11 more
wiley   +1 more source