Results 71 to 80 of about 3,983,345 (293)

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Comparative Evaluation of Three Preprocessing Methods for Extraction and Detection of Influenza A Virus Nucleic Acids from Sputum

Frontiers in Medicine, 2018
Viscous sputum specimens usually cannot undergo automated extraction, and thus, a pre-homogenization process is desirable before isolating nucleic acids for real-time reverse transcription PCR.
Fei Yu, Ting Qiu, Ying Zeng, Yiyin Wang, Shufa Zheng, Xiao Chen, Yu Chen   +6 more
doaj   +1 more source

Direct electrophoretic microRNA preparation from clinical samples using nanofilter membrane

Nano Convergence, 2020
A method to directly collect negatively charged nucleic acids, such as DNA and RNA, in the biosamples simply by applying an electric field in between the sample and collection buffer separated by the nanofilter membrane is proposed.
Kidan Lee, Jae-Hyun Kang, Hyun-Mi Kim, Junhyoung Ahn, Hyungjun Lim, JaeJong Lee, Wan-Jin Jeon, Jae-Hoon Lee, Ki-Bum Kim   +8 more
doaj   +1 more source

Fluorinated peptide nucleic acid [PDF]

, 2003
The fluorinated olefinic peptide nucleic acid analogue (F-OPA) monomer containing the base thymine was synthesised in 13 steps. PNAs containing this unit were prepared and their pairing properties assessed by means of UV-melting ...
Marcel Hollenstein, Gautschi, Daniel, Daniel Gautschi, Leumann, Christian, Hollenstein, Marcel, Christian J. Leumann, Leumann CJ, Gautschi D, Hollenstein M   +8 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Drug-nucleic acid interactions /

, 2001
This volume consolidates the key methods for studying ligand-nucleic acid interactions into a convenient source. Techniques that are examined range from biophysical and chemical approaches to methods rooted in molecular and cell biology.Includes ...
Chaires, Jonathan B.,editor., Waring, Michael J.,editor.   +1 more
core  

Data for: Impact of nucleic acid extraction platforms on hepatitis virus genome detection

, 2019
Data used for figures in the manuscript " Impact of nucleic acid extraction platforms on hepatitis virus genome detection"
Pauly, M (via Mendeley Data), Pauly, Matthew   +1 more
core   +1 more source

Design, synthesis, conformational analysis and nucleic acid hybridisation properties of thymidyl pyrrolidine-amide oligonucleotide mimics (POM) [PDF]

, 2003
Pyrrolidine-amide oligonucleotide mimics (POM) 1 were designed to be stereochemically and conformationally similar to natural nucleic acids, but with an oppositely charged, cationic backbone.
Tan, T. H. Samuel, Morral, Jordi, Tan, T.H.S., Morral-Cardoner, Jordi, Micklefield, Jason, Cooper, M.A., Hickman, D. T., Micklefield, J., Morral, J., King, Paul M., Hickman, David T., Cooper, Matthew A., King, P.M., Hickman, D.T., Samuel Tan, T. H.   +14 more
core   +1 more source

Using cell‐free RNA to identify B‐ and T‐cell clonality for diagnosis and monitoring of B‐ and T‐cell neoplasms

FEBS Open Bio, EarlyView.
Using peripheral blood for determining B‐cell or T‐cell clonality is more reliable when we use cell‐free RNA (cfRNA) because cells release blood significantly more RNA than DNA. Next‐generation sequencing (NGS) of cfRNA allows us to evaluate fragment cfRNA and evaluate clonality reliably without the need for prior determination of the specific dominant
Adam Albitar, Sally Agersborg, Ahmed Charifa, Pooja Phull, Noa Biran, David Vesole, Harsh Parmar, Andrew Pecora, Andrew Ip, Andre Goy, David Siegel, Maher Albitar   +11 more
wiley   +1 more source

Highly Efficient Extraction of the Cellular Nucleic Acid Associated Protein in Vitro with Magnetic Oxidized Carbon Nanotube

, 2013
Highly Efficient Extraction of the Cellular Nucleic Acid Associated Protein in Vitro with Magnetic Oxidized Carbon ...
卫晓鸾, 秦洪强, 刘芳洁, 胡争艳, 吴仁安, 邹汉法, 张轶, 程凯   +7 more
core