Results 121 to 130 of about 225,030 (341)

Recent developments in the characterization of nucleic acid hybridization kinetics. [PDF]

Curr Opin Biomed Eng, 2021
Chen YI, Sripati MP, Nguyen TD, Chang YJ, Kuo YA, Hong S, Yeh HC.   +6 more
europepmc   +1 more source

Unlocking the Potential of MXene‐Based Electrochemical Biosensors: A Review of Biofunctionalization Strategies and Biosensing Principles

Advanced Materials Technologies, EarlyView.
ABSTRACT Electrochemical biosensors enable the accurate and timely detection of clinical biomarkers, improving healthcare and precision medicine. MXene nanosheets, a class of 2D transition metal carbides, nitrides, and carbonitrides, are promising materials for developing next‐generation electrochemical biosensors due to their unique physicochemical ...
Muhsin Ali, Sharat Chandra Barman, Isobel Steer, Aya H. Alshareef, Dana Alsulaiman   +4 more
wiley   +1 more source

A quantitative model predicts how m⁶A reshapes the kinetic landscape of nucleic acid hybridization and conformational transitions. [PDF]

Nat Commun, 2021
Liu B, Shi H, Rangadurai A, Nussbaumer F, Chu CC, Erharter KA, Case DA, Kreutz C, Al-Hashimi HM.   +8 more
europepmc   +1 more source

A new approach to understanding T cell development: the isolation and characterization of immature CD4-, CD8-, CD3- T cell cDNAs by subtraction cloning [PDF]

, 1992
During T cell development in the mammalian thymus, immature T cells are observed that lack the cell surface markers CD4, CD8, and CD3. A subtracted cDNA library was constructed to isolate cDNAs that are specific for these immature T cells.
Gese, Elaine, Hood, Leroy, Orr, Sally L.
core  

Chronic Hypoxia Disrupts Spermatogenesis Through ASXL2–EZH2–Mediated Microtubule Destabilization

Advanced Science, EarlyView.
This study reveals the mechanism by which chronic hypoxia impairs spermatogenesis via the ASXL2–EZH2 axis, hindering the transition of spermatids from round to elongated forms. Key findings reveal that under hypoxic conditions, downregulated ASXL2 expression reduces EZH2 binding to the CEP162 promoter, leading to decreased H3K27me3 modification and ...
Jun Yin, Mengjie Zhang, Wenying Liu, Wenlong Shen, Debao Li, Hongming Miao, Fang Deng, Gang Zhang, Yi Tian, Yi Zhang, Zhihu Zhao, Bing Ni   +11 more
wiley   +1 more source

Effect of channel length on the electrical response of carbon nanotube field-effect transistors to deoxyribonucleic acid hybridization

Beilstein Journal of Nanotechnology, 2014
A single-walled carbon nanotube (SWCNT) in a field-effect transistor (FET) configuration provides an ideal electronic path for label-free detection of nucleic acid hybridization.
Hari Krishna Salila Vijayalal Mohan, Jianing An, Yani Zhang, Chee How Wong, Lianxi Zheng   +4 more
doaj   +1 more source

Cisplatin-Digoxigenin mRNA Labeling for Nonradioactive Detection of mRNA Hybridized onto Nucleic Acid cDNA Arrays

BioTechniques, 1999
We optimized a novel nonradioactive hybridization technique using a cis-platin coupled digoxigenin derivative for direct labeling of mRNA. This new mRNA reporter molecule was applied to cDNA membrane arrays to simultaneously identify expression of ...
T. Hoevel, H. Holz, M. Kubbies
doaj   +1 more source

Stochastic Nanoscale Biophysical Cues as a Basis for the Induction of Glioblastoma‐Like Transcriptional Programs in Astrocytes

Advanced Science, EarlyView.
Stochastic nanoscale physical cues induce glioblastoma (GBM)‐associated transcriptional traits in naïve astrocytes leading to spontaneous formation of spheroids. Cells within spheroids express activated‐MMP2 and a differential gene expression pattern involving P53 and NOTCH3, providing evidence for a role for changes in brain topography, as observed in
Laurent Starck, Tobias Butelmann, Sabrina Hogan, Melika Sarem, Bernd Heimrich, Ritwick Sawarkar, Marie‐Françoise Ritz, Gregor Hutter, V. Prasad Shastri   +8 more
wiley   +1 more source

New Tools for Oligonucleotide Fingerprinting

BioTechniques, 2001
Oligonucleotide fingerprinting is an attractive, high-throughput complement to tag sequencing methods to determine the spectrum and abundance of genes in cDNA libraries.
A. Guerasimova, L. Nyarsik, I. Girnus, M. Steinfath, W. Wruck, H. Griffiths, R. Herwig, C. Wierling, J. O’Brien, H. Eickhoff, H. Lehrach, U. Radelof   +11 more
doaj   +1 more source

Murine cytomegalovirus: detection of latent infection by nucleic acid hybridization technique [PDF]

, 1980
K S Cheung, Erich Huang, David J. Lang
openalex   +1 more source