Results 201 to 210 of about 471,867 (378)

TopCas: Topology‐Gated Cas12a via DNA‐RNA Chimeric Circular crRNA for Amplification‐Free Nucleic Acid Detection and Conditional Gene Editing

Advanced Science, EarlyView.
The TopCas platform utilizes an engineered DNA‐RNA chimeric circular crRNA to establish topology‐gated control over CRISPR‐Cas12a activity, enabling target‐triggered self‐catalytic amplification (preamplification‐free). This system seamlessly integrates highly sensitive molecular diagnostics with conditionally controlled gene editing, demonstrating ...
Shun Zhang, Wen Sun, Ting Xiao, Yali Wang, Xianlan Wu, Huiyou Chen, Ming Chen, Jun Zhang   +7 more
wiley   +1 more source

Nucleic acid hybridization-based detection of pathogenic RNA using microscale thermophoresis. [PDF]

J Biol Chem
Avivi MY, Touitou N, Rohana H, Lerrer B, Shav-Tal Y, Peretz A, Cohen HY.   +6 more
europepmc   +1 more source

Electrochemical detection of hybridization using peptide nucleic acids and methylene blue on self-assembled alkanethiol monolayer modified gold electrodes

, 2002
Dilsat Ozkan‐Ariksoysal, Arzum Erdem, Pınar Kara, Kağan Kerman, J. Justin Gooding, Peter Nielsen, Mehmet Özsöz   +6 more
openalex   +2 more sources

Characterization of Gazdar murine sarcoma virus by nucleic acid hybridization and analysis of viral expression in cells [PDF]

, 1977
R H Pang, Leo A. Phillips, D. K. Haapala
openalex   +1 more source

SDS‐CRISPR for Single‐Nucleotide Variant Detection

Advanced Science, EarlyView.
Structure‐disruption‐sensitive CRISPR (SDS‐CRISPR) converts structural instability into single‐nucleotide precision, thereby overcoming mismatch tolerance in canonical Cas12a and enabling versatile diagnostics across DNA, RNA, and microRNA targets. When applied to rapid IDH1 mutation detection for glioma genotyping and integrated with lateral‐flow ...
Xin Guan, Chong Guo, Jiongyu Zhang, Rui Yang, Yerramsetti Ramachandra, Chengyu Hou, Minjie Pei, Shuo Zhang, Kurt T. Schalper, Xingye Liu, Qian Wu, Ketan R. Bulsara, Changchun Liu   +12 more
wiley   +1 more source

Multidimensional and Multifunctional Laser‐Induced Graphene (LIG) for Point‐of‐Care and Wearable Biosensing, Theranostics, and Bioactive Interfaces Toward Personalized Healthcare and Regenerative Medicine

Advanced Science, EarlyView.
Multidimensional laser‐induced graphene (LIG) spanning from 0D to 3D architectures is comprehensively reviewed for multifunctional biomedical platforms, including biosensing, theranostics, and bioactive interface applications, which highlights its potentials for point‐of‐care diagnostics, wearable health monitoring, smart drug delivery, and tissue ...
Li Zhang, Yurou Yuan, Xiaohong Ding, Wing Cheung Mak   +3 more
wiley   +1 more source

Characterization of multispecies biofilms by peptide nucleic acid fluorescence in situ hybridization (PNA-FISH)

, 2010
Carina Almeida, M. J. Vieira, C. W. Keevil, Rosa Maria Castilhos Fernandes, Nuno F. Azevedo   +4 more
openalex   +1 more source

Enhanced Intracellular Stability and Translation Efficiency of mRNA Drugs by a 2‐arm mRNA Platform

Advanced Science, EarlyView.
We constructed a 2‐arm mRNA, characterized by a unique topology formed through the dimerization of two mRNA 3’ tails. The 2‐arm mRNA improves 3’ tail stability and resistance to nuclease degradation, resulting in an intracellular half‐life of up to 65 h. This method substantially enhances the translation capacity of mRNA drugs.
Xucong Teng, Jiahao Lin, Qiushuang Zhang, Xiangdong Zhang, Yicong Dai, Jinghong Li   +5 more
wiley   +1 more source

Myxoid smooth muscle neoplasia of the uterus: comprehensive analysis by next-generation sequencing and nucleic acid hybridization [PDF]

, 2019
Ju‐Yoon Yoon, Adrián Mariño‐Enríquez, Natalie Stickle, Richard de Borja, Nadia Ismiil, Bojana Djordjevic, Carl Virtanen, A. Ravat, Marisa R. Nucci, Jelena Mirković, Carlos Parra‐Herran   +10 more
openalex   +1 more source

DNA Nanoarray for Multiplexed RNA Detection With Single‐Molecule Readout

Advanced Science, EarlyView.
A carrier‐based DNA nanoarray integrates programmable nucleic acid hybridization with solid‐state nanopore readout for direct RNA detection at single‐molecule resolution. A ternary encoding system enables 27 carriers with 81 addressable sensing sites, allowing the simultaneous identification of targets spanning a wide size range, from short microRNAs ...
Yunxuan Li, Yesheng Wang, Chunmiao Yu, Siyu Hou, Elli Mylona, Plamena Naydenova, Jingyuan Zhao, Hong Yuan, Jingsong Duan, Hendrik F. P. Runge, Stephen Baker, Jinbo Zhu, Ulrich F. Keyser   +12 more
wiley   +1 more source