Results 291 to 300 of about 225,030 (341)
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Nucleic acid hybridization in viral Hepatitis research
La Ricerca in Clinica e in Laboratorio, 1988Nucleic acid hybridization is extremely important in viral hepatitis research and the newly emerging techniques are now becoming an indispensable resource also in the diagnostic laboratory. This paper provides information on methods currently used for detection of viral nucleic acids with special emphasis on the importance of hepatitis B virus DNA in ...
Maran E +4 more
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Fluorescent Nucleic Acid Hybridization Methods.
SPIE Proceedings, 1989In the last several years a number of approaches have been developed that permit detection of nucleic acid hybridization using fluorescence techniques. These have several advantages over the previously available radioactive procedures. Among these are much higher spatial resolution, the speed with which the results are available, the reduced hazard ...
D. Pinkel +7 more
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New Developments in Nucleic Acid Hybridization
1985Nucleic acid hybridization is widely used for scientific applications in specialized laboratories. This paper describes hybridization probes that can be prepared with less specialized equipment. A new indirect 'sandwich' hybridization test is described which allows the use of only one universally usable labelled probe for hybridization tests with ...
H, Wolf +9 more
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Nucleic acid hybridization in viral diagnosis
Clinical Biochemistry, 1990Since 1982, numerous studies have been published utilizing a variety of hybridization techniques to detect viral nucleic acid directly in clinical specimens and in tissue sections. However, hybridization techniques are still not widely used in the clinical laboratory.
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Hybridization of Nucleic Acids to Chromosomes
1972Ever since the method of molecular hybridization of nucleic acids was conceived, a number of biologists have tried to hybridize labeled RNA or DNA to chromosomes for detection by autoradiography. The first report was by French and Kitzmiller (1967), who hybridized 3H-DNA from Drosophila melanogaster to the DNA of the salivary chromosomes of the same ...
D M, Steffensen, D E, Wimber
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Microfluidic chip for fast nucleic acid hybridization
Lab on a Chip, 2003The design and experimental verification of a fast nucleic acid hybridization microchip using the fluidic velocity and strain rate effects was conducted. This hybridization chip was able to increase the hybridization signal 6-fold, reduce non-specific target-probe binding and background noise within 30 min, as compared to conventional hybridization ...
Yung-Chiang, Chung +3 more
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Nucleic Acid Hybridization: Triplex Stability and Energetics
Annual Review of Biophysics and Biomolecular Structure, 1995In this chapter, we review the current state of the thermodynamic database for triple helical oligonucleotide hybridization reactions and present a critical assessment of the methods used to obtain the relevant data. The thermodynamic stability of triple-helix oligonucleotide constructs is discussed in terms of its dependence on temperature, chain ...
G E, Plum +3 more
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Nucleic Acid Hybridization Procedures
2019The application of nucleic acid probes and related techniques is becoming common in the detection of human and other animal viruses but has lagged somewhat in plant virology. The explosion in the use of the technology associated with recombinant DNA research has led to the development of various techniques by which viruses can be diagnosed.
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Hybridization of Nucleic Acids
1969I am not sure there is a true difference in DNA-DNA hybridization between DNA from young and old animals. This is a complicated question because of difficulties in preparing DNA completely free of protein, the great complexity of mammalian DNA compared to bacterial DNA, and the greater probability for non specific inter an intra molecular interaction ...
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Identification of foodborne pathogens by nucleic acid hybridization
International Journal of Food Microbiology, 1991Nucleic acid hybridization methods have been developed and used to identify microorganisms in foods. Tests performed on mixed cultures save the time required to establish pure cultures. Enterotoxigenic or invasive strains of foodborne bacterial pathogens are detected with probes that identify genes responsible for virulence.
W E, Hill, S P, Keasler
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