High‐Throughput Microfluidic‐Mediated Assembly of Layer‐By‐Layer Nanoparticles
Advanced Functional Materials, EarlyView.The layer‐by‐layer (LbL) assembly of polymer films on nanoparticle (NP) surfaces is a promising technique for targeted drug delivery. Despite its success in preclinical mouse models, a current good manufacturing practices (cGMP) compliant, clinical‐scale production method has been lacking.
Ivan S. Pires +4 more
wiley +1 more source
DoubleHelix: nucleic acid sequence identification, assignment and validation tool for cryo-EM and crystal structure models. [PDF]
Nucleic Acids Res, 2023 Chojnowski G.
europepmc +1 more source
Monolithic centrifugal microfluidic platform for bacteria capture and concentration, lysis, nucleic-acid amplification, and real-time detection [PDF]
, 2009 We report the design, fabrication, and characterization of a polymer centrifugal microfluidic system for the specific detection of bacterial pathogens.
Barry, Thomas +5 more
core +1 more source
Lipid Nanoparticle‐Mediated CRISPR‐Cas13a Delivery for the Control of Bacterial Infection
Advanced Healthcare Materials, Volume 14, Issue 7, March 14, 2025.New formulations of lipid nanoparticles (LNPs) that can deliver nucleic acids to Gram‐negative bacteria are proposed to combat bacterial infection. The delivery of nucleic acids by LNPs is aided by LNP‐helpers which weaken the bacterial outer membrane. LNPs encapsulating the Cas13a/gRNA expression vector achieve an antibacterial effect in both in vivo ...
Bookun Kim +9 more
wiley +1 more source
Development of a bicistronic anti-CD19/CD20 CAR construct including abrogation of unexpected nucleic acid sequence deletions. [PDF]
Mol Ther Oncolytics, 2023 Lam N +10 more
europepmc +1 more source
NUCLEIC ACID SEQUENCE PHYLOGENY AND RANDOM OUTGROUPS
Cladistics, 1990 Abstract— When divergent taxa are used to root networks, it is assumed that the character stales in the outgroup have historical similarity to those in the ingroup. Yet, if the data are nucleic acid sequences, the character stales shared by a divergent outgroup may be based not on history but on random similarity. A simple procedure is proposed to test
openaire +4 more sources
Recent Applications of Mesoporous Silica Nanoparticles in Gene Therapy
Advanced Healthcare Materials, EarlyView.The review summarizes the synthesis of mesoporous silica nanoparticles (MSNs) with modifiable surface properties, functionalization strategies, mechanism of therapeutic payload release, and current applications in gene therapy, focusing on their capabilities in the targeted delivery of therapeutic nucleic acids, CRISPR‐Cas systems, and other genetic ...
Tamanna Binte Huq +4 more
wiley +1 more source
An improved nucleic acid sequence-based amplification method mediated by T4 gene 32 protein. [PDF]
PLoS One, 2022 Nai YH, Doeven EH, Guijt RM.
europepmc +1 more source
United States Patent Application Publication: COUNTER SELECTION STRATEGY FOR GRAM-NEGATIVE BACTERIA [PDF]
, 2006 A Gram-negative bacterium useful for genetically engineering plants is provided. The Gram-negative bacterium contains, as part of genome, an inducible regulatory sequence operatively linked to a nucleotide sequence encoding a levansucrase. Alternatively,
Clemente, Thomas E. +2 more
core +1 more source
Advanced Healthcare Materials, EarlyView.Negative‐ and positive‐staining TEM is essential for rapid nanometer‐resolution characterization of organic specimens ranging from nanoparticles to cells. Uranyl salts are widely used negative‐/positive‐stains but are radioactive and highly toxic to users and the environment.
Vera M. Kissling +4 more
wiley +1 more source