Results 271 to 280 of about 1,295,417 (401)

An Engineered PfAgo with Wide Catalytic Temperature Range and Substrate Spectrum

Advanced Science, EarlyView.
This work presents a strategy for engineering thermophilic Ago proteins to obtain wide catalytic temperature range and broad substrate spectrum. This strategy is applied to engineer PfAgo and TtdAgo, and the biochemical properties of the engineered PfAgo are systematically characterized. The study further elucidates the structural differences involved.
Longyu Wang, Xiaochen Xie, Fuyong Huang, Qiang Wei, Tianxin Cai, Na Yu, Shi Chen, Fei Wang, Wanping Chen, Chin‐yu Chen, Chunhua Li, Lixin Ma   +11 more
wiley   +1 more source

Visual detection and differentiation of Classic Swine Fever Virus strains using nucleic acid sequence-based amplification (NASBA) and G-quadruplex DNAzyme assay. [PDF]

Sci Rep, 2017
Lu X, Shi X, Wu G, Wu T, Qin R, Wang Y.
europepmc   +1 more source

Comparison between Quantitative Nucleic Acid Sequence-Based Amplification, Real-Time Reverse Transcriptase PCR, and Real-Time PCR for Quantification of Leishmania Parasites

Journal of Clinical Microbiology, 2007
W. F. van der Meide, J. Guerra, G. Schoone, M. Farenhorst, L. Coelho, W. Faber, Inge Peekel, H. Schallig   +7 more
semanticscholar   +1 more source

The hybridization-stabilization assay: a solution-based isothermal method for rapid screening and determination of sequence preference of ligands that bind to duplexed nucleic acids [PDF]

, 2001
Carlos González
openalex   +1 more source

Nanoparticle Delivery of Antisense miR162 Inhibits Invasive Habitat Adaption of Alternanthera Philoxeroides

Advanced Science, EarlyView.
This study demonstrates that the miRNA pathway plays a key role in the invasive growth of Alternanthera philoxeroides in aquatic habitats. Specifically, miR162 is critical for its invasive success, facilitating stem elongation and enhancing survival during submergence.
Qianqian Hu, Erfeng Kou, Xiuzhen Liao, Ruiyi Qiu, Qi Tang, Huan Zhang, Yun Zheng, Ji Yang, Binglian Zheng   +8 more
wiley   +1 more source

Real-Time Detection of Noroviruses in Surface Water by Use of a Broadly Reactive Nucleic Acid Sequence-Based Amplification Assay

Applied and Environmental Microbiology, 2006
S. Rutjes, H. van den Berg, W. Lodder, A. M. de Roda Husman   +3 more
semanticscholar   +1 more source

The Bioengineering of Microspheric Skin Organoids and Their Application in Drug Screening

Advanced Science, EarlyView.
A novel method for the preparation of uniform microspheric skin organoids via spinning bioreactors is developed. The organoid consists of a core‐shell structure to mimic the bilayered skin structure with the epidermis on the surface. For efficient drug screening, the organoid is equipped with a luciferase reporter to detect canonical Wnt signaling, and
Jundong Xie, Qingyang Yang, Yanan Zhang, Ke Zheng, Hongya Geng, Yaojiong Wu   +5 more
wiley   +1 more source

Detection of Rhinoviruses by Tissue Culture and Two Independent Amplification Techniques, Nucleic Acid Sequence-Based Amplification and Reverse Transcription-PCR, in Children with Acute Respiratory Infections during a Winter Season

Journal of Clinical Microbiology, 2006
K. Loens, H. Goossens, C. D. Laat, H. Foolen, P. Oudshoorn, S. Pattyn, P. Sillekens, M. Ieven   +7 more
semanticscholar   +1 more source

Evaluation of the NucliSens Basic Kit for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in Genital Tract Specimens Using Nucleic Acid Sequence-Based Amplification of 16S rRNA [PDF]

, 2001
James B. Mahony, Xin-Ming Song, S. Chong, Michael Faught, T. Salonga, J. Kapala   +5 more
openalex   +1 more source

Small RNA Toxin‐Assisted Evolution of GC‐Preferred ErCas12a for Enhanced Genome Targeting Range

Advanced Science, EarlyView.
ErCas12a is engineered to target GC‐rich PAMs using a small RNA toxin‐aided positive screening system. The resulting variant, enErCas12a, exhibits an expanded PAM profile and facilitates efficient gene editing in both bacterial and mammalian cells, while preserving high targeting specificity for both canonical and non‐canonical PAM targets.
Zehua Chen, Junyuan Xue, Ziying Wang, Jinyuan Sun, Yinglu Cui, Tong Zhu, Huaiyi Yang, Ming Li, Bian Wu   +8 more
wiley   +1 more source