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Sequence-Specific Sensing of Nucleic Acids [PDF]

Trends in Immunology, 2017
Innate immune cells are endowed with many nucleic acid receptors, but the role of sequence in the detection of foreign organisms remains unclear. Can sequence patterns influence recognition? In addition, how can we infer those patterns from sequence data?
Nicolas, Vabret, Nina, Bhardwaj, Benjamin D, Greenbaum   +2 more
openaire   +2 more sources

Next-generation fluorescent nucleic acids probes for microscopic analysis of intracellular nucleic acids

Applied Microscopy, 2019
Fluorescence imaging of nucleic acids is a very important technique necessary to understand gene expression and the resulting changes in cell function. This mini-review focuses on sequence-specific fluorescence imaging of intracellular RNA and methylated
Akimitsu Okamoto
doaj   +1 more source

A CRISPR-based nucleic acid detection method for severe fever with thrombocytopenia syndrome virus

Virus Research, 2022
Objective: Fever with thrombocytopenia syndrome virus (SFTS) is a tick-borne infection now known to spread among humans as an aerosol, which has resulted in several outbreaks across Asia over the past decade.
Yansong Zhang, Xuanyang Bai, Jinhui Li, Jing Xie, Huan Li, Lang Yang, Peihan Li, Peng Li, Hao Dong, Qichao Chen, Xinyan Hu, Yun Wang, Tingting Jiang, Hongbin Song, Leili Jia, Lizhong Li   +15 more
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Enlarged Similarity of Nucleic Acid Sequences [PDF]

DNA Research, 1996
The concept of nucleic acid sequence base alternations is presented. The number of base alterations for the sequences of different length is established. The definition of "enlarged similarity" of nucleic acids sequences on the basis of sequence base alterations is introduced.
E V, Korotkov, M A, Korotkova
openaire   +2 more sources

Improving the specificity of nucleic acid detection with endonuclease-actuated degradation

Communications Biology, 2022
A new method, SENTINEL, improves the specificity of nucleic acid target sequence identification via a combination of isothermal amplification and sequence-specific DNA endonucleases. The method can discriminate single base-pair differences and takes less
Roger S. Zou, Momcilo Gavrilov, Yang Liu, Dominique Rasoloson, Madison Conte, Justin Hardick, Leo Shen, Siqi Chen, Andrew Pekosz, Geraldine Seydoux, Yukari C. Manabe, Taekjip Ha   +11 more
doaj   +1 more source

DNAssist, a C++ Program for Editing and Analysis of Nucleic Acid and Protein Sequences on PC-Compatible Computers Running Windows 95, 98, NT4.0 or 2000

BioTechniques, 2000
The size of sequence databases, the rapid rate of generating sequence data and the need to routinely construct recombinant DNA molecules in molecular biological research necessitate the frequent handling and analysis of nucleotide and protein sequences ...
Hugh G. Patterton, Steven Graves
doaj   +1 more source

Mitigation of the effect of variability in digital PCR assays through use of duplexed reference assays for normalization

BioTechniques, 2018
Digital PCR has been promoted as a technique for obtaining absolute measures of the amount of nucleic acid target sequence in a sample, but still lacks standardization in data reporting.
Sherry J Coulter
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Nucleic Acid Probes for Single-Molecule Localization Imaging of Cellular Biomolecules

Chemical & Biomedical Imaging, 2023
Endogenous biomolecules in cells are the basis of all life activities. Directly visualizing the structural characteristics and dynamic behaviors of cellular biomolecules is significant for understanding the molecular mechanisms in various biological ...
Junyuan Wei, Cailing Ji, Yingfei Wang, Jie Tan, Quan Yuan, Weihong Tan   +5 more
doaj   +1 more source

Application of CRISPR/Cas Systems in the Nucleic Acid Detection of Infectious Diseases

Diagnostics, 2022
The CRISPR/Cas system is a protective adaptive immune system against attacks from foreign mobile genetic elements. Since the discovery of the excellent target-specific sequence recognition ability of the CRISPR/Cas system, the CRISPR/Cas system has shown
Junwei Li, Yuexia Wang, Bin Wang, Juan Lou, Peng Ni, Yuefei Jin, Shuaiyin Chen, Guangcai Duan, Rongguang Zhang   +8 more
doaj   +1 more source

CRISPR-induced DNA reorganization for multiplexed nucleic acid detection

Nature Communications, 2023
Nucleic acid sensing involving CRISPR technologies is powerful but has certain limitations, such as PAM sequence requirements and limited multiplexing.
Margot Karlikow, Evan Amalfitano, Xiaolong Yang, Jennifer Doucet, Abigail Chapman, Peivand Sadat Mousavi, Paige Homme, Polina Sutyrina, Winston Chan, Sofia Lemak, Alexander F. Yakunin, Adam G. Dolezal, Shana Kelley, Leonard J. Foster, Brock A. Harpur, Keith Pardee   +15 more
doaj   +1 more source