Results 51 to 60 of about 694,635 (336)

Decrease in gyrase A protein expression in _E. coli_ cells inhibited by antisense ribozymes [PDF]

, 2008
RNase P complexed with external guide sequence (EGS) represents a novel nucleic-acid-based gene interference approach to modulate gene expression. Nucleic acid-based gene interference technologies represent promising strategies for specific inhibition of
Malathi Raghunathan, Shilpakala Sainath Rao   +1 more
core   +1 more source

Evolutionary interplay between viruses and R‐loops

FEBS Letters, EarlyView.
Viruses interact with specialized nucleic acid structures called R‐loops to influence host transcription, epigenetic states, latency, and immune evasion. This Perspective examines the roles of R‐loops in viral replication, integration, and silencing, and how viruses co‐opt or avoid these structures.
Zsolt Karányi, Zita Képes, Zoltán Szabó, Eszter Csoma, Lóránt Székvölgyi   +4 more
wiley   +1 more source

SOFB is a comprehensive ensemble deep learning approach for elucidating and characterizing protein-nucleic-acid-binding residues

Communications Biology
Proteins and nucleic-acids are essential components of living organisms that interact in critical cellular processes. Accurate prediction of nucleic acid-binding residues in proteins can contribute to a better understanding of protein function.
Bin Zhang, Zilong Hou, Yuning Yang, Ka-chun Wong, Haoran Zhu, Xiangtao Li   +5 more
doaj   +1 more source

Purification tags markedly affect self‐aggregation of CPEB3

FEBS Letters, EarlyView.
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase   +6 more
wiley   +1 more source

Rational Design of Short Locked Nucleic Acid-Modified 2′-O-Methyl Antisense Oligonucleotides for Efficient Exon-Skipping In Vitro

Molecular Therapy: Nucleic Acids, 2017
Locked nucleic acid is a prominent nucleic acid analog with unprecedented target binding affinity to cDNA and RNA oligonucleotides and shows remarkable stability against nuclease degradation.
Bao T. Le, Abbie M. Adams, Susan Fletcher, Stephen D. Wilton, Rakesh N. Veedu   +4 more
doaj   +1 more source

Development of a Quantitative BRET Affinity Assay for Nucleic Acid-Protein Interactions. [PDF]

PLoS ONE, 2016
Protein-nucleic acid interactions play a crucial role in the regulation of diverse biological processes. Elucidating the roles that protein-nucleic acid complexes play in the regulation of transcription, translation, DNA replication, repair and ...
Timothy A Vickers, Stanley T Crooke
doaj   +1 more source

Method for detecting Ralstonia solanacearum race 3 biovar 2 [PDF]

, 2009
The invention concerns a method for the detection of Ralstonia solanacearum race 3 biovar 2 strains in a medium, comprising the determination of the presence or the absence in a sample of the medium, of: (i) at least one first nucleic acid target having ...
Boucher, Christian, Elbaz, Mounira, Genin, Stéphane, Guidot, Alice, Prior, Philippe   +4 more
core  

PubDNA Finder in a Nutshell. Searching the Life Sciences Literature with Sequences of Nucleic Acids [PDF]

, 2011
Biomedical researchers and clinicians working with molecular technologies in routine clinical practice often need to review the available literature to gather information regarding specific sequences of nucleic acids. This includes, for instance, finding
García Remesal, Miguel
core   +1 more source

Correlation between nucleotide composition and folding energy of coding sequences with special attention to wobble bases [PDF]

, 2008
Background: The secondary structure and complexity of mRNA influences its accessibility to regulatory molecules (proteins, micro-RNAs), its stability and its level of expression.
AA Komar, AA Komar, C Kimchi-Sarfaty, C Soares, C Workman, DE Draper, DJ Patterson, EG Shaper, EG Shpaer, F Pagani, GD Stormo, IM Meyer, J Duan, J Konecny, Jan C Biro, JC Biro, JC Biro, JC Biro, JC Biro, JC Biro, JD Lieb, JR Powell, JV Chamary, K Mita, K Robison, KB Nielsen, L Cartegni, L Katz, M Jia, M Kellis, M Oresic, M Oresic, M Zama, M Zuker, M Zuker, MD Ermolaeva, NR Markham, P Cortazzo, P Mukhopadhyay, S Itzkovitz, SA Shabalina, W Gu, W Gu, W Seffens, W Seffens, WC Winkler, ZE Sauna   +46 more
core   +4 more sources

Intron‐oriented HTLV‐1 integration in an adult T‐cell leukemia/lymphoma cell line sustains expression of intact ift81 mRNA

FEBS Letters, EarlyView.
In the adult T‐cell leukemia/lymphoma (ATL) cell line ED, the human T‐cell leukemia virus type 1 (HTLV‐1) provirus was integrated into the intron of the ift81 gene in the antisense orientation. Despite this integration, both the intact ift81 and the viral oncogene hbz were simultaneously expressed, likely due to the functional insufficiency of viral ...
Mayuko Yagi, Yuka Hirosawa, Yusaku Sagisaka, Minami Hama, Satoshi Takeda, Jun A. Komano   +5 more
wiley   +1 more source