Results 71 to 80 of about 1,249,290 (394)
ClampFISH detects individual nucleic-acid molecules using click chemistry based amplification
Nature Biotechnology, 2018 Methods for detecting single nucleic acids in cell and tissues, such as fluorescence in situ hybridization (FISH), are limited by relatively low signal intensity and nonspecific probe binding.
Sara H. Rouhanifard +12 more
semanticscholar +1 more source
FEBS Letters, EarlyView.Mutations in the C9orf72 gene represent the most common genetic cause of amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disease. Using patient‐derived neurons and C. elegans models, we find that the nucleoporin Nup107 is dysregulated in C9orf72‐associated ALS. Conversely, reducing Nup107 levels mitigates disease‐related changes.
Saygın Bilican +7 more
wiley +1 more source
Molecular Therapy: Nucleic Acids, 2017 Locked nucleic acid is a prominent nucleic acid analog with unprecedented target binding affinity to cDNA and RNA oligonucleotides and shows remarkable stability against nuclease degradation.
Bao T. Le +4 more
doaj +1 more source
Development of a Quantitative BRET Affinity Assay for Nucleic Acid-Protein Interactions. [PDF]
PLoS ONE, 2016 Protein-nucleic acid interactions play a crucial role in the regulation of diverse biological processes. Elucidating the roles that protein-nucleic acid complexes play in the regulation of transcription, translation, DNA replication, repair and ...
Timothy A Vickers, Stanley T Crooke
doaj +1 more source
Mitochondrial fatty acid oxidation is stimulated by red light irradiation
FEBS Letters, EarlyView.Light at different wavelengths has distinct effects on keratinocyte viability and metabolism. UVA light abrogates metabolic fluxes. Blue and green light have no effect on metabolic fluxes, while red light enhanced oxidative phosphorylation by promoting fatty acid oxidation. Keratinocytes are the primary constituents of sunlight‐exposed epidermis.
Manuel Alejandro Herrera +4 more
wiley +1 more source
Paradigms for computational nucleic acid design [PDF]
, 2004 The design of DNA and RNA sequences is critical for many endeavors, from DNA nanotechnology, to PCR‐based applications, to DNA hybridization arrays.
Dirks, Robert M. +3 more
core +5 more sources
FEBS Letters, EarlyView.In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley +1 more source
Nucleic Acid Ligands Based on Carbohydrates
CHIMIA, 1996 Sequence-specific nucleic acid ligands are important tools in chemistry and molecular biology and are thought to possess a considerable pharmaceutical potential. An overview of the structurally and mechanistically diverse approaches in the field
Jürg Hunziker
doaj +2 more sources
SNBRFinder: A Sequence-Based Hybrid Algorithm for Enhanced Prediction of Nucleic Acid-Binding Residues. [PDF]
PLoS ONE, 2015 Protein-nucleic acid interactions are central to various fundamental biological processes. Automated methods capable of reliably identifying DNA- and RNA-binding residues in protein sequence are assuming ever-increasing importance.
Xiaoxia Yang +3 more
doaj +1 more source
Targeting of the hepatitis B virus precore protein to the endoplasmic reticulum membrane: after signal peptide cleavage translocation can be aborted and the product released into the cytoplasm. [PDF]
, 1988 The major hepatitis B virus (HBV) core protein is a viral structural protein involved in nucleic acid binding. Its coding sequence contains an extension of 29 codons (the "precore" region) at the amino terminus of the protein which is present in a ...
Garcia, PD +3 more
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