Results 71 to 80 of about 376,743 (310)

Recent Advances in Nucleic Acid Targeting Probes and Supramolecular Constructs Based on Pyrene-Modified Oligonucleotides

Molecules, 2017
In this review, we summarize the recent advances in the use of pyrene-modified oligonucleotides as a platform for functional nucleic acid-based constructs.
Olga A. Krasheninina, Darya S. Novopashina, Evgeny K. Apartsin, Alya G. Venyaminova   +3 more
doaj   +1 more source

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site‐Specific Integration of CFTR and Mutation‐Agnostic Disease Rescue

Advanced Functional Materials, EarlyView.
Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley, Paul G. Ayoub, Vrishti Sinha, Colin Juett, Alicia Sanoyca, Emily C. Duggan, Lindsay E. Lathrop, Priyanka Bhatt, Kevin Coote, Beate Illek, Brigitte N. Gomperts, Donald B. Kohn, Steven J. Jonas   +12 more
wiley   +1 more source

Advances in nucleic acid probe-based detection of gene point mutations: a review

Frontiers in Chemistry
A fundamental characteristic of gene mutations is the permanent alteration of the DNA sequence, including point mutations, deletions, inversions, and translocations.
Xuyang Pu, Xuyang Pu, Xueqiang Wu, Xueqiang Wu, Xueqiang Wu, Xueqiang Wu   +5 more
doaj   +1 more source

FeDSNP‐Pa Nanoassemblies: A Triple‐Action Therapeutic Strategy Targeting Oxidative Stress, Inflammation, and Pyroptosis for Retinal Ganglion Cell Protection in Glaucoma

Advanced Functional Materials, EarlyView.
FeDSNP‐Pa, a metallized nanoparticle loaded with sodium pyruvate (Pa), exerts triple therapeutic effects by scavenging reactive oxygen species (ROS), suppressing inflammatory responses, and inhibiting pyroptosis signaling pathways. This multifunctional neuroprotective strategy protecting retinal ganglion cells (RGCs) from elevated intraocular pressure ...
Yukun Wu, Xue Li, Tong Wu, Dan Ji, Yugang Bai, Xuezhi Zhou   +5 more
wiley   +1 more source

FBPP: software to design PCR primers and probes for nucleic acid base detection of foodborne pathogens

Scientific Reports
Foodborne pathogens can be found in various foods, and it is important to detect foodborne pathogens to provide a safe food supply and to prevent foodborne diseases.
Mohamed A. Soliman, Mohamed S. Azab, Hala A. Hussein, Mohamed M. Roushdy, Mohamed N. Abu el-naga   +4 more
doaj   +1 more source

Sequence programmable nucleic acid coacervates

AbstractNature uses bottom-up self-assembly to build structures with remarkable complexity and functionality. Understanding how molecular-scale interactions translate to macroscopic properties remains a major challenge and requires systems that effectively bridge these two scales.
Sumit Majumder, Sebastian Coupe, Nikta Fakhri, Ankur Jain   +3 more
openaire   +2 more sources

CRISPR/Cas9‐Assisted Microrobots for Fast and Ultrasensitive “On‐The‐Fly” Next‐Generation DNA Detection

Advanced Functional Materials, EarlyView.
This work presents self‐propelled CRISPR/Cas9‐functionalized Au–MRs for rapid, amplification‐free, “on‐the‐fly” DNA detection. By harnessing motion‐assisted signal recovery, the platform achieved the limit of detection in low fM DNA concentrations, enabling detection across a wide dynamic range within only 5 min, which is significantly faster than any ...
Jyoti, Petr Michálek, Zbyněk Heger, Martin Pumera   +3 more
wiley   +1 more source

Isothermal Amplification Methods for the Detection of Nucleic Acids in Microfluidic Devices

Biosensors, 2012
Diagnostic tools for biomolecular detection need to fulfill specific requirements in terms of sensitivity, selectivity and high-throughput in order to widen their applicability and to minimize the cost of the assay.
Giuseppe Spoto, Laura Maria Zanoli
doaj   +1 more source

Machine Learning Guides Peptide Nucleic Acid Flow Synthesis and Sequence Design [PDF]

, 2021
Chengxi Li, Genwei Zhang, Somesh Mohapatra, Alex J. Callahan, Andrei Loas, Rafael Gómez‐Bombarelli, Bradley L. Pentelute   +6 more
openalex   +1 more source

Selective and Precise Editing of Digital Polymers Through Parallel or Series Toehold‐Mediated Strand Displacement

Advanced Functional Materials, EarlyView.
A sequence‐encoded supramolecular construct containing two accessible toeholds is developed herein for enabling multiple editing operations. By introducing specific input strands, it is possible to selectively erase or rewrite digital content through parallel or series toehold‐mediated strand displacement (PTMSD or STMSD).
Jakub Ossowski, Cyril Antheaume, Jean‐François Lutz, Maria Nerantzaki   +3 more
wiley   +1 more source