Results 81 to 90 of about 709,713 (351)

By-product formation in repetitive PCR amplification of DNA libraries during SELEX. [PDF]

PLoS ONE, 2014
The selection of nucleic acid aptamers is an increasingly important approach to generate specific ligands binding to virtually any molecule of choice. However, selection-inherent amplification procedures are prone to artificial by-product formation that ...
Fabian Tolle, Julian Wilke, Jesper Wengel, Günter Mayer   +3 more
doaj   +1 more source

An extra dimension in nucleic acid sequence recognition

Quarterly Reviews of Biophysics, 2005
Introduction 312Triple helices in DNA 312Chemically modified TFOs 313Further development 316Recognition of GC base pairs 316Recognition of TA base pairs 316Recognition of AT base pairs 317Recognition of CG base pairs 317RNA triplexes 317Kinetics of triplex formation 318Practical applications of triplexes 318Conclusions 319References 319Watson–Crick ...
Fox, Keith R., Brown, Tom
openaire   +4 more sources

Identifying Interaction Sites in "Recalcitrant" Proteins: Predicted Protein and Rna Binding Sites in Rev Proteins of Hiv-1 and Eiav Agree with Experimental Data [PDF]

, 2005
Protein-protein and protein nucleic acid interactions are vitally important for a wide range of biological processes, including regulation of gene expression, protein synthesis, and replication and assembly of many viruses.
Carpenter, Susan, Dobbs, Drena, Dobbs, Drena, Honavar, Vasant, Jernigan, Robert, Jernigan, Robert, Lee, Jae-Hyung, Terribilini, Michael, Yan, Changhui   +8 more
core   +3 more sources

Identification of antisense nucleic acid hybridization sites in mRNA molecules with self-quenching fluorescent reporter molecules [PDF]

, 2005
We describe a physical mRNA mapping strategy employing fluorescent self-quenching reporter molecules (SQRMs) that facilitates the identification of mRNA sequence accessible for hybridization with antisense nucleic acids in vitro and in vivo, real time ...
Do, Bao T., Gewirtz, Alan M., Gifford, Lida K., Greenham, Paul, Jordan, David, Kalota, Anna, Lu, Ponzy, Opalinska, Joanna B., Pattanayak, Vikram, Robbins, Michelle, Rodriguez, Lesbeth C., Vernovsky, Kathy   +11 more
core   +2 more sources

Multi‐omic characterization of consensus molecular subtype 1 (CMS1) colorectal cancer with dampened immune response improves precision medicine

Molecular Oncology, EarlyView.
This study highlights the importance of multi‐omic analyses in characterizing colorectal cancers. Indeed, our analysis revealed a rare CMS1 exhibiting dampened immune activation, including reduced PD‐1 expression, moderate CD8+ T‐cell infiltration, and suppressed JAK/STAT pathway.
Livia Concetti, Manuel Scimeca, Julia Bischof, Jonathan Woodsmith, Massimiliano Agostini, Cristina Fiorani, Yufang Shi, Eleonora Candi, Gerry Melino, Alessandro Mauriello, Giuseppe S. Sica   +10 more
wiley   +1 more source

Protein-RNA interactions: a structural analysis [PDF]

, 2001
A detailed computational analysis of 32 protein-RNA complexes is presented. A number of physical and chemical properties of the intermolecular interfaces are calculated and compared with those observed in protein-double-stranded DNA and protein-single ...
Berman, HM, Daley, DT, Jones, S, Luscombe, NM, Thornton, JM   +4 more
core   +2 more sources

Targeting the AKT/mTOR pathway attenuates the metastatic potential of colorectal carcinoma circulating tumor cells in a murine xenotransplantation model

Molecular Oncology, EarlyView.
Dual targeting of AKT and mTOR using MK2206 and RAD001 reduces tumor burden in an intracardiac colon cancer circulating tumor cell xenotransplantation model. Analysis of AKT isoform‐specific knockdowns in CTC‐MCC‐41 reveals differentially regulated proteins and phospho‐proteins by liquid chromatography coupled mass spectrometry. Circulating tumor cells
Daniel J. Smit, Thais Pereira‐Veiga, Helena Brauer, Michael Horn, Paula Nissen, Thomas Mair, Bente Siebels, Hannah Voß, Ruimeng Zhuang, Marie‐Therese Haider, Desiree Loreth, Margarita Iskhakova, Bele Lindemann, Julian Kött, Laure Cayrefourcq, Jasmin Wellbrock, Hartmut Schlüter, Klaus Pantel, Catherine Alix‐Panabières, Manfred Jücker   +19 more
wiley   +1 more source

Characterization of Aptamer-Protein Complexes by X-ray Crystallography and Alternative Approaches [PDF]

, 2012
Aptamers are oligonucleotide ligands, either RNA or ssDNA, selected for high-affinity binding to molecular targets, such as small organic molecules, proteins or whole microorganisms.
Baugh, Bauke W. Dijkstra, Bing, Bock, Cao, Chayen, Convery, Doudna, Ellington, Friedmann, Garber, Hauke Smidt, Hermann, Hianik, Hoggan, Hollis, Horn, Huang, Huang, Hwang, Jiang, Johan Hekelaar, John van der Oost, Kaur, Ke, Kelly, Kikin, Krauss, Kwan, Laing, Lebruska, Lee, Long, Lupold, Macaya, Mark Levisson, Mascini, McPherson, Mehta, Miyakawa, Moorthy, Murai, Nix, Nomura, Orlova, Padmanabhan, Padmanabhan, Paige, Parisien, Poniková, Reinemann, Reinstein, Renault, Rivas, Rowsell, Ruigrok, Sekiya, Shum, Skrzypczak-Jankun, Snyder, Someya, Stoltenburg, Sugiyama, Sussman, Tereshko, Tuerk, Vincent J. B. Ruigrok, Wang, Wilson, Win, Wochner, Yan, Yee, Zuker   +73 more
core   +6 more sources

Chemoresistome mapping in individual breast cancer patients unravels diversity in dynamic transcriptional adaptation

Molecular Oncology, EarlyView.
This study used longitudinal transcriptomics and gene‐pattern classification to uncover patient‐specific mechanisms of chemotherapy resistance in breast cancer. Findings reveal preexisting drug‐tolerant states in primary tumors and diverse gene rewiring patterns across patients, converging on a few dysregulated functional modules. Despite receiving the
Maya Dadiani, Gilgi Friedlander, Gili Perry, Nora Balint‐Lahat, Shlomit Gilad, Dana Morzaev‐Sulzbach, Anjana Shenoy, Noa Bossel Ben‐Moshe, Anya Pavlovsky, Rinat Bernstein‐Molho, Eytan Domany, Iris Barshack, Tamar Geiger, Bella Kaufman, Einav Nili Gal‐Yam   +14 more
wiley   +1 more source

Analysis and Annotation of Nucleic Acid Sequence [PDF]

, 1998
The aims of this project were to develop improved methods for computational genome annotation and to apply these methods to improve the annotation of genomic sequence data with a specific focus on human genome sequencing. The project resulted in a substantial body of published work.
openaire   +5 more sources