Results 91 to 100 of about 737,876 (305)

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site‐Specific Integration of CFTR and Mutation‐Agnostic Disease Rescue

Advanced Functional Materials, EarlyView.
Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley, Paul G. Ayoub, Vrishti Sinha, Colin Juett, Alicia Sanoyca, Emily C. Duggan, Lindsay E. Lathrop, Priyanka Bhatt, Kevin Coote, Beate Illek, Brigitte N. Gomperts, Donald B. Kohn, Steven J. Jonas   +12 more
wiley   +1 more source

Discovery, Design, Synthesis, and Application of Nucleoside/Nucleotides

Molecules, 2020
For decades, nucleosides and nucleotides have formed the cornerstone of antiviral, antiparasitic and anticancer therapeutics and have been used as tools in exploring nucleic acid structure and function [...]
Katherine Seley-Radtke
doaj   +1 more source

FeDSNP‐Pa Nanoassemblies: A Triple‐Action Therapeutic Strategy Targeting Oxidative Stress, Inflammation, and Pyroptosis for Retinal Ganglion Cell Protection in Glaucoma

Advanced Functional Materials, EarlyView.
FeDSNP‐Pa, a metallized nanoparticle loaded with sodium pyruvate (Pa), exerts triple therapeutic effects by scavenging reactive oxygen species (ROS), suppressing inflammatory responses, and inhibiting pyroptosis signaling pathways. This multifunctional neuroprotective strategy protecting retinal ganglion cells (RGCs) from elevated intraocular pressure ...
Yukun Wu, Xue Li, Tong Wu, Dan Ji, Yugang Bai, Xuezhi Zhou   +5 more
wiley   +1 more source

Genetic interactions of G-quadruplexes in humans

eLife, 2019
G-quadruplexes (G4) are alternative nucleic acid structures involved in transcription, translation and replication. Aberrant G4 formation and stabilisation is linked to genome instability and cancer.
Katherine G Zyner, Darcie S Mulhearn, Santosh Adhikari, Sergio Martínez Cuesta, Marco Di Antonio, Nicolas Erard, Gregory J Hannon, David Tannahill, Shankar Balasubramanian   +8 more
doaj   +1 more source

Robustness and modularity properties of a non-covalent DNA catalytic reaction [PDF]

, 2009
The biophysics of nucleic acid hybridization and strand displacement have been used for the rational design of a number of nanoscale structures and functions. Recently, molecular amplification methods have been developed in the form of non-covalent DNA
Aldaye, Clo, D. Y. Zhang, E. Winfree, Feldkamp, Flamm, Frezza, Gao, Lubrich, Marras, Puglisi, SantaLucia, Seelig, Seelig, Turberfield, Yin, Yurke, Zhang, Zhang, Zhang, Zuker   +20 more
core   +4 more sources

Synergistic Osteogenesis After Co‐Administration of cmRNAs Encoding BMP‐2 and BMP‐7 Utilizing a Transcript‐Activated Matrix

Advanced Functional Materials, EarlyView.
This study demonstrates that the dual delivery of BMP‐2/‐7 coding cmRNAs for bone healing is demonstrated as feasible, safe, and highly osteogenic. Compared to single BMP‐2 or BMP‐7 cmRNAs, the combination enhances the production of both mineral and organic components of the extracellular matrix when delivered using a collagen‐HA scaffold, supporting ...
Claudia Del Toro Runzer, Joanna M. Sadowska, Nadia Roumans, Christian Plank, Christopher H. Evans, Fergal J. O'Brien, Martijn van Griensven, Elizabeth R. Balmayor   +7 more
wiley   +1 more source

Reading More than Histones: The Prevalence of Nucleic Acid Binding among Reader Domains

Molecules, 2018
The eukaryotic genome is packaged into the cell nucleus in the form of chromatin, a complex of genomic DNA and histone proteins. Chromatin structure regulation is critical for all DNA templated processes and involves, among many things, extensive post ...
Tyler M. Weaver, Emma A. Morrison, Catherine A. Musselman   +2 more
doaj   +1 more source

Influence of a fluorobenzene nucleobase analogue on the conformational flexibility of RNA studied by molecular dynamics simulations [PDF]

, 2006
Chemically modified bases are frequently used to stabilize nucleic acids, to study the driving forces for nucleic acid structure formation and to tune DNA and RNA hybridization conditions.
Engels, Joachim W. (Prof. Dr.), Zacharias, Martin   +1 more
core  

A mutate-and-map protocol for inferring base pairs in structured RNA

, 2013
Chemical mapping is a widespread technique for structural analysis of nucleic acids in which a molecule's reactivity to different probes is quantified at single-nucleotide resolution and used to constrain structural modeling.
CS Chow, D Moazed, DH Mathews, EE Regulski, JB Lucks, JG Underwood, JP Staley, KA Wilkinson, M Kertesz, M Mandal, N Kulshina, NB Leontis, P Cordero, P Cordero, P Rocca-Serra, P Tijerina, PJ Lukavsky, R Rydzanicz, RC Spitale, RF Gesteland, S Mitra, S Washietl, S Yoon, SA Mortimer, SE Wells, SM Vasa, SW Brostoff, VD Sood, W Kladwang, W Kladwang, W Kladwang, W Kladwang, Y Rhee   +32 more
core   +1 more source

Mechanisms of HIV-1 Nucleocapsid Protein Inhibition by Lysyl-Peptidyl-Anthraquinone Conjugates [PDF]

, 2016
The Nucleocapsid protein NCp7 (NC) is a nucleic acid chaperone responsible for essential steps of the HIV-1 life cycle and an attractive candidate for drug development.
Alice Sosic, Barbara Gatto, Cristina Parolin, Dan Fabris, Giuseppe Zagotto, Giuseppina Sabatino, Guo J., Ilaria Carli, Laura Sinigaglia, Marta Cappellini, Morellet N., Paolo Rovero, You J. C.   +12 more
core   +2 more sources