In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS
FEBS Letters, EarlyView.We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka +11 more
wiley +1 more source
Exponentially fitted numerical method for solving singularly perturbed delay reaction-diffusion problem with nonlocal boundary condition. [PDF]
BMC Res Notes, 2023 Wondimu GM +3 more
europepmc +1 more source
Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions
FEBS Letters, EarlyView.We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva +10 more
wiley +1 more source
Cell wall target fragment discovery using a low‐cost, minimal fragment library
FEBS Letters, EarlyView.LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan +5 more
wiley +1 more source
An efficient numerical method on modified space-time sparse grid for time-fractional diffusion equation with nonsmooth data. [PDF]
Numer Algorithms, 2023 Zhu BY, Xiao AG, Li XY.
europepmc +1 more source
FEBS Letters, EarlyView.Bone metastasis in prostate cancer (PCa) patients is a clinical hurdle due to the poor understanding of the supportive bone microenvironment. Here, we identify stearoyl‐CoA desaturase (SCD) as a tumor‐promoting enzyme and potential therapeutic target in bone metastatic PCa.
Alexis Wilson +7 more
wiley +1 more source
Numerical analysis of MICP treated sand based on bio-chemo-hydro model
Frontiers in Bioengineering and BiotechnologyMicrobially Induced Calcite Precipitation (MICP) represents an environmentally friendly and innovative soil grouting technology. Involving intricate biochemical processes, it poses challenges for a thorough investigation of factors influencing microbial ...
Delong Li, Shengzhe Chen, Xing Gao
doaj +1 more source
An efficient numerical method for a singularly perturbed Fredholm integro-differential equation with integral boundary condition. [PDF]
J Appl Math Comput, 2023 Durmaz ME, Amirali I, Amiraliyev GM.
europepmc +1 more source
FEBS Letters, EarlyView.We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee +3 more
wiley +1 more source
A mathematical model and mesh-free numerical method for contact-line motion in lubrication theory. [PDF]
Environ Fluid Mech (Dordr), 2022 Pang KE, Ó Náraigh L.
europepmc +1 more source