Results 71 to 80 of about 922,998 (286)

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

Application of Exact Newton Optimisation to the Maximum Likelihood Ensemble Filter

Tellus: Series A, Dynamic Meteorology and Oceanography
The Newton method is used for optimisation in the maximum likelihood ensemble filter (MLEF) to improve analysis convergence and accuracy. The proposed method is compared against the original method using the conjugate gradient (CG) method preconditioned ...
Takeshi Enomoto, Saori Nakashita
doaj   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Self-supporting structure design in additive manufacturing through explicit topology optimization

, 2017
One of the challenging issues in additive manufacturing (AM) oriented topology optimization is how to design structures that are self-supportive in a manufacture process without introducing additional supporting materials. In the present contribution, it
Du, Zongliang, Guo, Xu, Liu, Chang, Liu, Ying, Zhang, Weisheng, Zhou, Jianhua   +5 more
core   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Numerical Optimization Methods for Metasurfaces

Laser & Photonics Reviews, 2020
AbstractIn recent years, metasurfaces have emerged as revolutionary tools to manipulate the behavior of light at the nanoscale. These devices consist of nanostructures defined within a single layer of metal or dielectric materials, and they offer unprecedented control over the optical properties of light, leading to previously unattainable applications
Elsawy, Mahmoud, Lanteri, Stéphane, Duvigneau, Régis, Fan, Jonathan, Genevet, Patrice   +4 more
openaire   +3 more sources

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Solving the inverse problem of high numerical aperture focusing using vector Slepian harmonics and vector Slepian multipole fields

, 2012
A technique using vector Slepian harmonics and multipole fields is presented for a general treatment of the inverse problem of high numerical aperture focusing.
Bokor, Braat, Braat, Chen, Chen, Devaney, Foreman, Jabbour, Jahn, Jahn, Kant, Kant, Kornél Jahn, Levy, Nándor Bokor, Sheppard, Sherif, Török, Wang, Wang, Wang, Wolf, Zhao   +22 more
core   +1 more source

The neural crest‐associated gene ERRFI1 is involved in melanoma progression and resistance toward targeted therapy

Molecular Oncology, EarlyView.
ERRFI1, a neural crest (NC)‐associated gene, was upregulated in melanoma and negatively correlated with the expression of melanocytic differentiation markers and the susceptibility of melanoma cells toward BRAF inhibitors (BRAFi). Knocking down ERRFI1 significantly increased the sensitivity of melanoma cells to BRAFi.
Nina Wang, Qian Sun, Daniel Novak, Lei Zhu, Juliane Poelchen, Tamara Steinfass, Yiman Wang, Viktor Umansky, Jochen Utikal   +8 more
wiley   +1 more source

Numerical Optimization of the Coil Geometry in a Large-Scale Levitation Melting Device—With Titanium as a Case Study

Energies
Electromagnetic levitation melting offers the opportunity for the energy-efficient processing of reactive and high-purity metals. This paper concerns a new levitator design that significantly expands the achievable mass of molten metal by processing a ...
Sławomir Golak, Radosław Zybała
doaj   +1 more source