Results 141 to 150 of about 343,769 (301)

Do efficacy results obtained from randomized controlled trials translate to effectiveness data from observational studies for relapsing–remitting multiple sclerosis? [PDF]

Background: Randomized controlled trials are considered the gold standard in regulatory decision making, as observational studies are known to have important methodological limitations.
Nikolakopoulos, Stavros, Elferink, Andre J., Zhou, Guiling, Ahmed, Wouter, Rengerink, Katrien Oude, Mol, Peter G.M., Hak, Eelko, Verweij, Stefan, Bijlsma, Maarten J., Mavridis, Dimitris   +9 more
core  

The Possibilities of Observational Research in Workplace Learning: The Case of Video-Reflexive Ethnography

Objective This paper, using video-reflexive ethnography (VRE) as a case study, explores the prospects for and possibilities of observational research in workplace learning.
Billett, Stephen, O’Shannessy, Megan, Furness, Linda, Ajjawi, Rola, Carrigan, Brendan, Noble, Christy, Teodorczuk, Andrew   +6 more
core   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

A Framework For Observational Data-Based Response Surface Methodology [PDF]

Response Surface Methodology (RSM) is an integrated tool for optimization purposes based on an experiment. The designed experiment ensures the researcher fully controls all factors that potentially influence the response and simultaneously fulfills the ...
Wibowo, Budhi Sholeh, Hadiyat, Mochammad Arbi, Sopha, Bertha Maya   +2 more
core  

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Erythropoietin modulates hepatic inflammation, glucose homeostasis, and soluble epoxide hydrolase and epoxides in high‐fat diet‐induced obese mice

FEBS Open Bio, EarlyView.
Erythropoietin administration suppresses hepatic soluble epoxide hydrolase (sEH) expression, leading to increased CYP‐derived epoxides. This is associated with a shift in hepatic macrophage polarization characterized by reduced M1 markers and increased M2 markers, along with reduced hepatic inflammation, suppressed hepatic lipogenesis, and attenuated ...
Takeshi Goda, Satoru Sugimoto, Chiharu Cho, Madoka Konishi, Nozomi Inoue, Satoshi Miyagaki, Yasuhiro Kawabe, Takuro Okamura, Masahide Hamaguchi, Hisakazu Nakajima, Michiaki Fukui, Masakazu Shinohara, Tomoko Iehara   +12 more
wiley   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

The specification of the propensity score in multilevel observational studies

Propensity Score Matching (PSM) has become a popular approach to estimation of causal effects. It relies on the assumption that selection into a treatment can be explained purely in terms of observable characteristics (the “unconfoundedness assumption ...
Mealli, Fabrizia, Arpino, Bruno
core  

D-optimal plans in observational studies [PDF]

This paper investigates the use of Design of Experiments in observational studies in order to select informative observations and features for classification.
Weihs, Claus, Pumplün, Constanze, Morik, Katharina, Rüping, Stefan   +3 more
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source