Results 141 to 150 of about 1,578,105 (322)
FEBS Open Bio, EarlyView.Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
Anchorage‐independent and faster growth in clonal population from UV‐irradiated NER‐deficient cells
FEBS Open Bio, EarlyView.UV‐irradiated cells expressing a DDB2 mutant protein unable to interact with PCNA (DDB2PCNA‐) form clones able to grow without anchorage. Different experimental approaches reveal heterogeneity in cell cycle regulation and drug response within these clones, emphasizing the crucial role of the DDB2‐PCNA interaction in preventing cellular transformation ...
Paola Perucca +6 more
wiley +1 more source
Pensamiento. Revista de Investigación e Información Filosófica, 2013 So far we can identify at least three concepts within modern cosmology that bring into debate the question of consciousness in the universe: 1) Fine Tuning; 2) The Anthropic Principle and 3) The Multiverse.
Khalil Chamcham
doaj
MachinesMotor temperature prediction is critical for ensuring the reliability and safe operation of high-power-density electric drives. Since direct measurement of internal temperatures, especially rotor and magnet temperatures, is often impractical, virtual ...
Yaofei Han +6 more
doaj +1 more source
SAGE Open, 2016 It has been an accepted scientific fact in physics for almost 100 years that time speeds up and slows down for an observer based on factors—such as motion and gravity—that affect space.
Lucian Gideon Conway +2 more
doaj +1 more source
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains
FEBS Open Bio, EarlyView.The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth +6 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Epistemological Problems of Quantum Mechanics Following Ernst Cassirer and Richard Hönigswald
RUDN Journal of PhilosophyThis article deals with the Copenhagen interpretation of quantum mechanics and its epistemological discussion by Ernst Cassirer and Richard Hönigswald. The starting point is Cassirer's treatise Determinism and Indeterminism in Modern Physics, published ...
Reinhold Breil
doaj +1 more source