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Site-directed mutagenesis of odorant-binding proteins

2020
Modifying the affinity of odorant-binding proteins (OBPs) to small ligands by replacement of specific residues in the binding pocket may lead to several technological applications. Thanks to their compact and stable structures, OBPs are currently regarded as the best candidates to be used in biosensing elements for odorants and volatiles detection. The
Jiao, Zhu   +4 more
openaire   +2 more sources

Odorant-Binding Proteins from a Primitive Termite

Journal of Chemical Ecology, 2002
Hitherto, odorant-binding proteins (OBPs) have been identified from insects belonging to more highly evolved insect orders (Lepidoptera, Coleoptera, Diptera, Hymenoptera, and Hemiptera), whereas only chemosensory proteins have been identified from more primitive species, such as orthopteran and phasmid species.
Yuko, Ishida   +3 more
openaire   +2 more sources

Structure and biotechnological applications of odorant-binding proteins

Applied Microbiology and Biotechnology, 2013
Odorant-binding proteins (OBPs) are small soluble polypeptides found in sensory organs of vertebrates and insects as well as in secretory glands and are dedicated to detection and release of chemical stimuli. OBPs of vertebrates belong to the family of lipocalin proteins, while those of insects are folded into α-helical domains.
Pelosi, Paolo   +4 more
openaire   +4 more sources

Subtypes of odorant‐binding proteins

European Journal of Biochemistry, 1998
Odorant‐binding proteins (OBP) in the mucus of the olfactory epithelium are thought to transfer the hydrophobic odorous compounds through the aqueous barrier towards the chemo‐sensory cells. To evaluate their binding properties, two distinct OBP subtypes of the rat were expressed as N‐terminal His‐tagged fusion proteins in Escherichia coli, thus ...
Lobel D   +4 more
openaire   +2 more sources

Antennal transcriptome analysis of the maize weevil Sitophilus zeamais: Identification and tissue expression profiling of candidate odorant-binding protein genes.

Archives of Insect Biochemistry and Physiology, 2019
Our bioassays reviewed that antennae played crucial roles in the responses of maize weevil (Sitophilus zeamais) to food and sex volatiles. In order to identify the maize weevil odorant-binding protein (OBP) genes, we analyzed its antennal transcriptome ...
Q. Tang   +5 more
semanticscholar   +1 more source

Evidence of an Odorant-Binding Protein in the Human Olfactory Mucus:  Location, Structural Characterization, and Odorant-Binding Properties

Biochemistry, 2002
Odorant-binding proteins (OBPs) are small abundant extracellular proteins belonging to the lipocalin superfamily. They are thought to participate in perireceptor events of odor detection by carrying, deactivating, and/or selecting odorant molecules. Putative human OBP genes (hOBP) have recently been described [Lacazette et al. (2000) Hum. Mol. Genet. 9,
Briand, Loïc   +8 more
openaire   +3 more sources

Expression patterns and ligand binding characterization of Plus-C odorant-binding protein 14 from Adelphocoris lineolatus (Goeze).

Comparative Biochemistry and Physiology Part B Comparative Biochemistry, 2019
Odorant-binding proteins (OBPs) can bind and transport hydrophobic odorants across the sensillum lymph to the olfactory receptors (ORs) and play crucial roles in insect chemosensory systems.
Liang Sun   +6 more
semanticscholar   +1 more source

Chemical structure of semiochemicals and key binding sites together determine the olfactory functional modes of odorant-binding protein 2 in Eastern honey bee, Apis cerana.

International Journal of Biological Macromolecules, 2019
Insects can exhibit flexible olfaction that is sensitive to complex natural chemical environments. Odorant-binding proteins (OBPs) in insects' antennal chemosensilla can act as transporters of plant volatiles and pheromones across the sensillar lymph ...
Hongliang Li   +6 more
semanticscholar   +1 more source

Ligand-binding properties of odorant-binding protein 6 in Athetis lepigone to sex pheromones and maize volatiles.

Pest Management Science, 2021
Lu-Lu Li   +9 more
semanticscholar   +1 more source

Molecular and Functional Characterization of One Odorant-Binding Protein Gene OBP3 in Bemisia tabaci (Hemiptera: Aleyrodidae)

Journal of Economic Entomology, 2019
Odorant binding proteins (OBPs) of insects play a critical role in chemical perceptions and choice of insect host plant. Bemisia tabaci is a notorious insect pest which can damage more than 600 plant species.
Ran Wang   +4 more
semanticscholar   +1 more source

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