Results 181 to 190 of about 144,244 (266)

Impact of Agitation Power Input on Aspergillus oryzae Metabolism: Application of Multi‐Omics on an Industrial Enzyme Fermentation

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT The agitation power input substantially impacts the oxygen transfer rate in aerobic fermentation processes. Furthermore, in a carbon‐limited process where the substrate feed rate is controlled by the concentration of dissolved oxygen, the power input will consequently govern the feed rate.
Mariana Albino   +6 more
wiley   +1 more source

Magnetoelastic Cell Monitoring System for Evaluating the Effects of Surface Chemistry on the Performance of Mesenchymal Stem Cell Growth

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT The recent clinical adoption of cell therapies has increased the need for more efficient, robust, and reproducible manufacturing technologies. Human mesenchymal stromal cells (hMSCs), a major component of the cell and immunotherapeutic market, remain challenging to manufacture at scale because they require surface attachment and are highly ...
William S. Skinner   +7 more
wiley   +1 more source

Continuous Purification of mRNA Produced by In Vitro Transcription Using High Performance Countercurrent Membrane Purification

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT Messenger ribonucleic acid (mRNA) therapeutics produced by in vitro transcription must be purified to remove residual enzymes and free nucleotides. This study examines the use of high‐performance countercurrent membrane purification (HPCMP) for the purification of mRNA therapeutics based on differences in the rate of diffusion across ...
Ziqiao Wang   +3 more
wiley   +1 more source

Real‐Time Biomass Estimation in High‐Density Yeast Fermentations Using Soft Sensor Modeling

open access: yesBiotechnology and Bioengineering, EarlyView.
Accurate biomass measurements, both offline and online, are essential to improve prediction and control in yeast fermentation. This study develops regression‐based predictive models to correlate offline measurements (Dry Cell Weight and OD600 from a spectrophotometer) with online OD860 probe signals to provide accurate real‐time biomass estimations and
Ana G. Del Hierro   +3 more
wiley   +1 more source

Model‐Enabled Knowledge Transfer Across Cell Lines, Culture Scales and Conditions

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT Mechanistic models are central to quantitative understanding and optimization of Chinese hamster ovary (CHO) cell culture processes, but their utility is often restricted by parameter sets calibrated for specific cell lines, scales, or operating conditions.
Luxi Yu   +2 more
wiley   +1 more source

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