Results 71 to 80 of about 278,165 (247)

Genome-wide functional screens enable the prediction of high activity CRISPR-Cas9 and -Cas12a guides in Yarrowia lipolytica

Nature Communications, 2022
The successful use of CRISPR-based mutagenesis in non-conventional microorganisms requires high activity sgRNAs. Here, the authors present DeepGuide, a neural network-based architecture, that learns from genome-wide CRISPR activity profiles to accurately
Dipankar Baisya, Adithya Ramesh, Cory Schwartz, Stefano Lonardi, Ian Wheeldon   +4 more
doaj   +1 more source

Fatty acid ethyl esters production in aqueous phase by the oleaginous yeast Rhodosporidium toruloides

, 2013
Fatty acid ethyl esters (FAEEs) are attractive biofuel molecules. Conventional FAEEs production process uses triglycerides and ethanol as feedstocks and is sensitive to water contents.
Jin, Guojie, 赵宗保, Shen, Hongwei, Yang, Xiaobing, Zhao, Zongbao K., Zhang, Yixin, Xie, Haibo   +6 more
core   +1 more source

Carbohydrate‐Mediated Cellular Uptake of Boronic Acid Hybrids In Cellulo and Insulin‐Deficient Zebrafish: Live Imaging and Application of Multiphoton FLIM

Advanced Science, EarlyView.
Boronic acid‐based fluorescent hybrids have been used as part of a versatile platform for real‐time metabolic bioimaging across cellular and organismal scales. Including glucose‐responsive imaging in living cells and in an insulin‐deficient vertebrate model, this work opens new opportunities for the development of precision diagnostics, metabolic ...
Haobo Ge, John S. Fossey, David Gonzalez Calatayud, Stephen E. Flower, Sabrina Wang, Hanna Aitessaid, Melita Tvardauskaite, Julia Dudzic, Max Jewell, Charareh Pourzand, Stanley W. Botchway, David Gurevich, Tony D. James, Sofia I. Pascu   +13 more
wiley   +1 more source

RNA interference in the oleaginous yeast Rhodosporidium toruloides

, 2019
The red yeast Rhodosporidium toruloides is an excellent microbial host for production of carotenoids, neutral lipids and valuable enzymes. In recent years, genetic tools for gene expression and gene disruption have been developed for this red yeast ...
Zhang, Qi, Liu, Hongdi, Zhang, Yue, Zhao, Zongbao Kent, Liu, Xiangjian, Jiao, Xiang, Zhang, Sufang   +6 more
core   +2 more sources

Single‐Molecule Imaging of Endogenous RNA Using a Miniaturized and Circularly Permuted CRISPR‐Cas13 Degron‐Masking System CDegSR

Advanced Science, EarlyView.
CDegSR, a new tool for imaging RNA at single molecule level in living cells was developed by researchers. Background noise was eliminated by protein engineering and making it degrade when not bound to its target. This allowed individual RNA molecules to be clearly imaged and tracked.
Shipeng Shao, Hongchen Zhang
wiley   +1 more source

Engineering the oleaginous yeast Yarrowia lipolytica for industrial biosustainability [PDF]

, 2017
The conversion of renewable biomass to industrial chemicals of interest through microbial synthesis represents a clean sustainable alternative to the finite supply of dirty fossil fuels that modern society is heavily dependent on.
Yu, James
core   +1 more source

Microbial Lipid Production with Oleaginous Yeasts [PDF]

, 2014
Oleaginous microorganisms are able to convert carbon sources into storage lipids which are suited for diverse industrial applications as alternative for plant oil, fossil fuels and fish oil.
Schulze, Ines
core   +1 more source

Biosynthesis of Spatially Differentiated Cellulose for Flexible All‐in‐one Supercapacitors

Advanced Science, EarlyView.
Spatially differentiated bacterial cellulose is grown by oxygen‐guided biosynthesis to form dense surface regions connected by an open fibrillar interlayer. This architecture supports electroactive material loading, ion transport, and mechanical stability in an all‐in‐one supercapacitor, showing how biological assembly can create device‐ready ...
Xin Shu, Yi Lu, Xuetong Shi, Zhangmin Wan, Peijin Jiang, Minke Yang, Qingshi Tu, Orlando J. Rojas   +7 more
wiley   +1 more source

Trans-omic analysis of the oleaginous yeast Rhodosporidium toruloides

, 2012
Trans-omic analysis of the oleaginous yeast Rhodosporidium ...
赵宗保, 朱志伟, 张素芳, 刘宏伟, 邹汉法   +4 more
core  

Oil production on wastewaters after butanol fermentation by oleaginous yeast Trichosporon coremiiforme

, 2012
From the distillation process after butanol fermentation, wastewaters mainly consisted of organic acids and residual sugars and with high COD (usually >20,000 mg/L) are generated. Without any pretreatment and adding other nutrients (nitrogen sources
Ma, Long-long, Huang, Chao, Chen, Xue-fang, Chen, Xin-de, Xiong, Lian, Chen, Yong   +5 more
core   +1 more source