Results 141 to 150 of about 131,505 (250)

The FsrA‐Mediated Iron‐Sparing Response Regulates the Biosynthesis of the Epipeptide EPE in Bacillus subtilis

Molecular Microbiology, EarlyView.
Under iron‐limited conditions, FsrA base‐pairs with the intergenic region between epeX and epeE, enhancing epeE translation and triggering EPE production. Toxin‐mediated cell lysis releases nutrients, including iron, which can be taken up by surviving cells.
Sarah Miercke, Rabea Ghandour, Kai Papenfort, Thorsten Mascher   +3 more
wiley   +1 more source

Efficiency of Digital Photolithographic Synthesis of Large, High-Quality DNA Libraries and Microarrays using a Guanine O⁶ Dephosphitylation Strategy. [PDF]

Commun Chem
Santhosh S, Istvánffy S, Sabary O, Yaakobi E, Giridhar M, Behr J, Somoza MM.   +6 more
europepmc   +1 more source

Expanded Functional Characterization and Optimization of Protein Expression in Treponema denticola Shuttle Plasmids

Molecular Oral Microbiology, EarlyView.
We report optimization of the Treponema denticola shuttle plasmid system by minimizing its size and by characterizing an array of promoter‐gene constructs for genetic complementation, including the first use in Treponema of a Tet‐inducible system for controlled expression of potentially toxic genes.
M. Paula Goetting‐Minesky, Valentina Godovikova, Prakaimuk Saraithong, Alexander H. Rickard, Brigette R. Crawley, Sara M. Agolli, Reagan L. Boyce, Trishna L. Appaji, J. Christopher Fenno   +8 more
wiley   +1 more source

Fluorescence-Based Detection of <i>KRAS</i> Mutations in Genomic DNA Using Magnetic Bead-Coupled LDR Assay. [PDF]

Methods Protoc
Morimoto C, Hashimoto M.
europepmc   +1 more source

Complex engineering of Solanum alkaloids structural diversity in Nicotiana benthamiana

Plant Biotechnology Journal, EarlyView.
Summary Transient expression in Nicotiana benthamiana offers a powerful and versatile platform for rapid production of complex specialized metabolites. Steroidal glycoalkaloids (SGAs) and steroidal saponins produced by members of the Solanaceae family are known for their diverse structures and activities including antimicrobial, anticancer, antiviral ...
Sachin A. Gharat, Adam Jozwiak, Ilana Rogachev, Elinor A. Sharon, Sagit Meir, Ashok P. Giri, Asaph Aharoni   +6 more
wiley   +1 more source

EBSn, a Robust Synthetic Reporter for Monitoring Ethylene Responses in Plants

Plant Biotechnology Journal, EarlyView.
ABSTRACT Ethylene is a gaseous plant hormone that controls a wide array of physiologically relevant processes, including plant responses to biotic and abiotic stress, and induces ripening in climacteric fruits. To monitor ethylene in plants, analytical methods, phenotypic assays, gene expression analysis and transcriptional or translational reporters ...
Josefina‐Patricia Fernandez‐Moreno, Mario Fenech, Anna E. Yaschenko, Chengsong Zhao, Matthew Neubauer, Hannah N. Davis, Alex J. Marchi, Raine Concannon, Alexandra Keren‐Keiserman, Moshe Reuveni, Victor Levitsky, Dmitry Oshchepkov, Vladislav Dolgikh, Alexander Goldshmidt, José T. Ascencio‐Ibáñez, Elena Zemlyanskaya, Jose M. Alonso, Anna N. Stepanova   +17 more
wiley   +1 more source

OpenIDS2: A low-cost, 3D-printed, open-source platform for reproducible construction of DNA microarray synthesizers. [PDF]

PLoS One
Kim J, Kim H, Bang D.
europepmc   +1 more source

Rare Natural SNP Activates SHOOT APICAL MERISTEM ENLARGER1 to Increase Branch Number and Silique Number on the Main Inflorescence in Brassica napus

Plant Biotechnology Journal, EarlyView.
ABSTRACT Brassica napus is the second most important oil crop worldwide. Number of primary branches (Branch number, BN) and silique number on the main inflorescence (SMI) are key yield‐related quantitative traits. Here, we cloned a major QTL, qDB.A09, which simultaneously influences BN and SMI.
Sihao Zhang, Bao Li, Xiaoting Li, Song Yu, Yebitao Yang, Peipei Liu, Tanglingdian He, Wei Wang, Mei Li, Liang Guo, Jinxing Tu   +10 more
wiley   +1 more source

Development and validation of a 70K SNP genotyping array for Atlantic halibut (Hippoglossus hippoglossus). [PDF]

BMC Genomics
Sinani VK, Wilson RC, Nome T, Acharya U, Kommisrud E, Wetten M, Kent MP.   +6 more
europepmc   +1 more source

A Branched SNF1‐Related Protein Kinase 2 Signalling Cascade Controls ABA‐Induced Ethylene Production and Regulates Both Fruit Ripening and Reproductive Growth

Plant Biotechnology Journal, EarlyView.
ABSTRACT Control of fruit ripening, quality and yield is of major scientific, nutritional and commercial importance. The burst of ethylene (ET) production at the initiation of ripening is the most critical event controlling climacteric (CL) fruit ripening, yet little is known about how it is initiated.
Zhengrong Dai, Yating Li, Changsheng Zhai, Jie Li, Ya Zeng, Jinyao Ouyang, Bing He, Wei Wang, Pingyin Guan, Don Grierson, Kunsong Chen, Wensuo Jia   +11 more
wiley   +1 more source