Results 131 to 140 of about 80,761 (229)

Automatable Virtual Array Screening System for Rapid Analysis of Mitochondrial DNA Polymorphism [PDF]

, 2013
Campbell, Rowan Stewart, Automatable Virtual Array Screening System For Rapid Analysis of Mitochondrial DNA Polymorphism. Doctor of Philosophy (Biomedical Sciences), May, 2002, 156 pp., 11 tables, 48 illustrations, bibliography, 96 titles.
Campbell, Rowan Stewart
core   +1 more source

Dual Quantum Dot Molecular FRET Probes for Picomolar DNA Hexaplexing

Small Methods, EarlyView.
Multiplexed quantification of six different DNA targets from a single 200 µL sample at picomolar concentrations was accomplished by spectrotemporal detection of time‐resolved FRET from a single Tb‐complex donor to two quantum dot acceptors using one‐color excitation and two‐color detection.
Ruifang Su, Federico Pini, Nour Fayad, Kimihiro Susumu, Marta M. Natile, Igor L. Medintz, Thibault Gallavardin, Thomas J. Sørensen, Niko Hildebrandt   +8 more
wiley   +1 more source

FDX1 Depletion Activates CD8+ T Cell Antitumor Immunity by Promoting DMBT1 Secretion in Cuproptosis of Colorectal Cancer

Cancer Science, EarlyView.
Elesclomol‐induced cuproptosis potently activates both innate and adaptive antitumor immunity, especially CD8+ T cell immunity. FDX1 serves as a central regulator of CD8+ T cell functionality through DMBT1 downregulation during cuproptosis in colorectal cancer.
Jieqiong Wu, Zaixi Lian, Mingzhou Li, Xingtu Qin, Xiaoyu Lei, Lihua Yang, Lin Bing, Qiuyue Li, Lingyan Zhou, Zhiyang Ma, Xuemei Xu, Jinghao Huang, Zilong Chen, Lijie Chen, Lingjie Zhang, Zhiyuan Xiao, Xiangyu Jian, Xiaoqin Zhang, Yanqing Ding, Yaping Ye, Li Liang, Hongli Jiao, Shuyang Wang   +22 more
wiley   +1 more source

Impact of G‐CSF on Donor TCR Clonal Diversity and T Cell Function During Donor HSC Mobilisation

Cell Proliferation, EarlyView.
In this study, we found that G‐CSF mobilisation caused a reduction in TCR clonal diversity and down‐regulation of T‐cell function in donors. This may be one of the reasons for the low incidence of GVHD mediated by G‐CSF after allo‐HSCT. Source: Created in BioRender. Li https://BioRender.com/otownki.
Xinye Li, Zhixi Chen, Jie Chen, Xingwei Zhang, Jiamian Zheng, Zirui Liu, Meixue Pan, Yue Li, Bo Yu, Shaohua Chen, Jing Lai, Xianfeng Zha, Liye Zhong, Yikai Zhang, Yangqiu Li   +14 more
wiley   +1 more source

Variability in intracellular localization of D‐amino acid oxidase in choroid plexus epithelial cells

The FEBS Journal, EarlyView.
D‐amino acid oxidase (DAO) in choroid plexus epithelial cells (CPECs) shows vesicle‐like localization by histological and super‐resolution analyses. DAO colocalizes with peroxisomal, Golgi, endosomal, lysosomal, autophagosomal, and exosomal markers, indicating diverse subcellular distribution. This suggests DAO is transported within CPECs to metabolize
Koji Ono, Yuji Shishido, Toshiyuki Yamagishi, Kiyoshi Fukui   +3 more
wiley   +1 more source

Whole genome amplification for PGD and PND; molecular and a-CGH diagnosis [PDF]

, 2009
Whole genome amplification amplifies the entire genome in a few hours from samples of minimal DNA quantities, even from single cells. This may have many applications, especially in prenatal diagnosis, PGD and PGS. The hypothesis for chapter 3 was: Can
Glentis, S.
core  

Cryo‐EM structures of multiple‐peptide resistance factor (MprF) from Pseudomonas aeruginosa

The FEBS Journal, EarlyView.
Cryo‐EM analysis of MprF from Pseudomonas aeruginosa reveals a dimeric enzyme, distinct from Rhizobium homologues. The soluble GNAT domain contains a dynamic amphipathic helix (TTH) that adopts different positions in detergent micelle and nanodisc, relative to the membrane.
Shaileshanand Jha, Kutti R. Vinothkumar
wiley   +1 more source

Aptamer‐mediated outer membrane destabilization overcomes low permeability resistance in Pseudomonas aeruginosa

The FEBS Journal, EarlyView.
DNA aptamers, Apt 60 and Apt 173, target the BamA protein to compromise outer membrane integrity in Pseudomonas aeruginosa, making it susceptible to Azithromycin, an antibiotic that is otherwise poorly effective due to the low‐permeability membrane.
Rupany Selvam, Michelle Khai Khun Yap, Rafael Giacomazzi, Sérgio F. Sousa, Hock Siew Tan   +4 more
wiley   +1 more source

Enhancing CRISPR‐Cas12a base editing in plants with LbCas12a variants and introns

Journal of Integrative Plant Biology, EarlyView.
Intron optimization of LbCas12a‐RRV improves cytosine and adenine base editing efficiency in plants, supports multiplexed and double‐strand break‐free genome modification, and expands precise genome engineering tools for crop breeding and plant functional research. ABSTRACT Cytosine base editors (CBEs) and adenine base editors (ABEs) are powerful tools
Yanhao Cheng, Gen Li, Man Zhou, Rushil Mandlik, Doris Wang, Yiping Qi   +5 more
wiley   +1 more source

Divergent roles of ent‐kaurene oxidase paralogs in rice momilactone biosynthesis

Journal of Integrative Plant Biology, EarlyView.
Rice contains a five‐gene tandem array of genes encoding members of the ent‐kaurene oxidase (KO) family, with OsKOL4 and OsKOL5 playing divergent roles in momilactone metabolism, but both acting in the production of antifungal and antibacterial phytoalexins, which are important for disease resistance.
Zhibiao Wang, Yiling Feng, Leiming You, Meimei Xu, Kristin Helwig, Riqing Li, Si Nian Char, Bing Yang, Reuben J. Peters   +8 more
wiley   +1 more source